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1.发明申请公开(公告)号：US20100093018A1
公开(公告)日：2010-04-15
申请号：US12597661
申请日：2008-04-28
申请人： Xiaohong Hou , Mingjun Huang , Wengang Yang
发明人： Xiaohong Hou , Mingjun Huang , Wengang Yang
CPC分类号： C07K14/005 , C07K2319/20 , C12N2770/24222 , G01N33/5767 , G01N2333/183 , G01N2500/10
摘要： Cells expressing chimeric hepatitis C virus NS4A protein are disclosed, as are vectors and methods for preparing such cells and methods of using the cells and the chimeric proteins therein in screening assays for detecting compounds that alter the associative characteristics of NS4A proteins. Disclosed screening assays are suitable for use in high throughput screening of compound libraries to identify compounds with a exhibiting specific anti-viral activity against hepatitis C virus.
摘要翻译： 公开了表达嵌合丙型肝炎病毒NS4A蛋白的细胞，以及用于制备这种细胞的载体和方法以及其中使用细胞和嵌合蛋白的筛选测定法的方法，用于检测改变NS4A蛋白的缔合特性的化合物。 公开的筛选测定法适用于化合物文库的高通量筛选以鉴定具有针对丙型肝炎病毒的特异性抗病毒活性的化合物。
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2.发明授权公开(公告)号：US07101676B2
公开(公告)日：2006-09-05
申请号：US10339217
申请日：2003-01-09
IPC分类号： G01N33/53
CPC分类号： C07K14/005 , C12N2740/16222 , G01N33/56988 , G01N2500/02
摘要： The present invention relates to methods of identifying a molecule from a library of molecules that inhibits binding of human immunodeficiency virus nucleocapsid 7 polypeptide (NCp7) to an oligonucleotide which comprises admixing an NCp7 polypeptide with at one labeled HIV-1 psi-site oligonucleotide and an amount of the molecule to be tested under binding conditions; and determining the amount of oligonucleotide bound to the NCp7 polypeptide, wherein a decrease in the amount of oligonucleotide bound in the presence of the molecule compared with the amount of oligonucleotide bound in the absence of the molecule indicates that the molecule inhibits binding of NCp7 polypeptide to the oligonucleotide.
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