公开(公告)号：US08828958B2

公开(公告)日：2014-09-09

申请号：US11664052

申请日：2005-09-28

Applicant: Yin Chen ,  Xin Xing Tan

Inventor： Yin Chen ,  Xin Xing Tan

IPC: C07H21/04 ,  C12N15/11 ,  C12N15/00

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/16 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2330/31 ,  C12N2330/50

Abstract: The current invention is directed to oligonucleotide sequences isolated from a sequence designated rbl-1 [SEQ ID NO. 19] that either kill or inhibit growth, or prevent the production of endogenously expressed toxin, of microorganisms. These ssDNA sequences, identified through use of a screening method, appear to act as modulators of essential growth functions which may act at the level of triplex formation, antisense inhibition, or as aptamers that alter gene function. The sequences, referred to as minimum functional regions, or MFRs, are useful inter alia as therapeutic agents for treatment of sepsis and other pathologies caused by microorganisms such as sepsis and/or in which microorganisms are contributory agents.

Abstract translation: 本发明涉及从命名为rbl-1 [SEQ ID NO.1]的序列分离的寡核苷酸序列。 19]可以杀死或抑制微生物的生长，或阻止生成内源性表达的毒素。 通过使用筛选方法鉴定的这些ssDNA序列似乎可以作为必需生长功能的调节剂，其可以在三重形成，反义抑制水平或作为改变基因功能的适体的作用下起作用。 称为最小功能区域或MFR的序列尤其可用作治疗败血症的治疗剂和由诸如败血症和/或其中微生物是贡献剂的微生物引起的其它病症。

公开(公告)号：US20120151244A1

公开(公告)日：2012-06-14

申请号：US13296340

申请日：2011-11-15

Applicant: Curtis Ling ,  Xing Tan ,  Hyungjin Kim

Inventor： Curtis Ling ,  Xing Tan ,  Hyungjin Kim

IPC: G06F1/12

CPC classification number: H04W56/001 ,  G01S19/14 ,  G01S19/235 ,  G06F1/12 ,  H03L1/02 ,  H03L1/026

Abstract: Methods and systems for precise temperature and timebase ppm error estimation using multiple timebases may comprise measuring a coarse reading of a temperature corresponding to the plurality of timebases. The frequencies of the timebases may be compared to generate a fine reading of the temperature based, at least in part, on the coarse reading and the comparison of the frequencies with respect to models of temperature dependencies for each of the timebases. The timebases may be calibrated utilizing the generated fine reading. The plurality of timebases may comprise different order temperature dependencies. The models of temperature dependencies of each of the plurality of timebases may be updated based, at least in part, on the fine reading of the temperature corresponding to the plurality of timebases. A global navigation satellite system (GNSS) clock signal may be utilized periodically to improve the accuracy of the calibration of the plurality of timebases.

Abstract translation: 使用多个时基的精确温度和时基ppm误差估计的方法和系统可以包括测量对应于多个时基的温度的粗略读数。 可以比较时基的频率，至少部分地基于粗略读数和频率相对于每个时基的温度依赖性模型的比较而产生温度的精细读数。 可以使用生成的精细读数来校准时基。 多个时基可以包括不同的顺序温度依赖性。 至少部分地，可以基于对应于多个时间基的温度的精细读取来更新多个时基中的每一个的温度依赖性的模型。 周期性地可以利用全球导航卫星系统（GNSS）时钟信号来提高多个时基的校准精度。

公开(公告)号：US08775851B2

公开(公告)日：2014-07-08

申请号：US13296340

申请日：2011-11-15

Applicant: Curtis Ling ,  Xing Tan ,  Hyungjin Kim

Inventor： Curtis Ling ,  Xing Tan ,  Hyungjin Kim

IPC: G06F1/12 ,  G01S19/23 ,  H03L1/02

CPC classification number: H04W56/001 ,  G01S19/14 ,  G01S19/235 ,  G06F1/12 ,  H03L1/02 ,  H03L1/026

Abstract: Methods and systems for precise temperature and timebase ppm error estimation using multiple timebases may comprise measuring a coarse reading of a temperature corresponding to the plurality of timebases. The frequencies of the timebases may be compared to generate a fine reading of the temperature based, at least in part, on the coarse reading and the comparison of the frequencies with respect to models of temperature dependencies for each of the timebases. The timebases may be calibrated utilizing the generated fine reading. The plurality of timebases may comprise different order temperature dependencies. The models of temperature dependencies of each of the plurality of timebases may be updated based, at least in part, on the fine reading of the temperature corresponding to the plurality of timebases. A global navigation satellite system (GNSS) clock signal may be utilized periodically to improve the accuracy of the calibration of the plurality of timebases.

Abstract translation: 使用多个时基的精确温度和时基ppm误差估计的方法和系统可以包括测量对应于多个时基的温度的粗略读数。 可以比较时基的频率，至少部分地基于粗读和相对于每个时间基的温度依赖性模型的频率的比较来生成温度的精细读数。 可以使用生成的精细读数来校准时基。 多个时基可以包括不同的顺序温度依赖性。 至少部分地，可以基于对应于多个时间基的温度的精细读取来更新多个时基中的每一个的温度依赖性的模型。 周期性地可以利用全球导航卫星系统（GNSS）时钟信号来提高多个时基的校准精度。

公开(公告)号：US08889397B2

公开(公告)日：2014-11-18

申请号：US10574254

申请日：2004-06-03

Applicant: Yin Chen ,  Xin Xing Tan

Inventor： Yin Chen ,  Xin Xing Tan

IPC: C12N15/74 ,  C12N7/00 ,  C12N1/20 ,  C12N1/00 ,  C12Q1/68 ,  A61K48/00 ,  C12N15/113 ,  C12N15/10 ,  C12N15/11

CPC classification number: C12N15/113 ,  C07H21/04 ,  C12N15/1034 ,  C12N15/111 ,  C12N15/74 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/127 ,  C12N2320/11 ,  C12N2330/31 ,  C12Q1/6837 ,  C12Q2565/531

Abstract: A selectively inducible, single-stranded DNA (ssDNA) expression library, a method for constructing a ssDNA expression library, a method for screening ssDNA using the expression library, and a method for identifying ssDNA molecules that alter expression of bacterial and fungal gene(s) related to cell growth and toxin production and secretion. The screening library is used to, among other things, identify ODNs effective in stopping cell growth, killing bacteria or fungi, or preventing bacteria and/or fungi from synthesizing and secreting their toxins, and/or to discover ODNs effective in eukaryotic (e.g., mammalian) cells for targeted alteration of gene function. The library is also useful for identifying ssDNAs or ODNs that are used as therapeutic agents for, for instance, providing a method for treatment of bacterial infections such as sepsis.

Abstract translation: 选择性诱导型单链DNA（ssDNA）表达文库，构建ssDNA表达文库的方法，使用表达文库筛选ssDNA的方法以及鉴定改变细菌和真菌基因表达的ssDNA分子的方法 ）与细胞生长和毒素生成和分泌有关。 除了别的以外，筛选文库用于鉴别有效阻止细胞生长，杀死细菌或真菌，或阻止细菌和/或真菌合成和分泌其毒素的ODN，和/或发现在真核生物中有效的ODN（例如， 哺乳动物）细胞用于靶向改变基因功能。 该文库也可用于鉴定用作治疗剂的ssDNA或ODN，例如提供治疗细菌感染如败血症的方法。

公开(公告)号：US20080206154A1

公开(公告)日：2008-08-28

申请号：US11664052

申请日：2005-09-28

Applicant: Yin Chen ,  Xin Xing Tan

Inventor： Yin Chen ,  Xin Xing Tan

IPC: A61K31/713 ,  C07H21/02 ,  C12N15/66 ,  A61P31/00 ,  C07H21/04 ,  C12N5/00

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/111 ,  C12N2310/16 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2330/31 ,  C12N2330/50

Abstract: The current invention is directed to oligonucleotide sequences isolated from a sequence designated rbl-1 [SEQ ID NO. 19] that either kill or inhibit growth, or prevent the production of endogenously expressed toxin, of microorganisms. These ssDNA sequences, identified through use of a screening method, appear to act as modulators of essential growth functions which may act at the level of triplex formation, antisense inhibition, or as aptamers that alter gene function. The sequences, referred to as minimum functional regions, or MFRs, are useful inter alia as therapeutic agents for treatment of sepsis and other pathologies caused by microorganisms such as sepsis and/or in which microorganisms are contributory agents.

Abstract translation: 本发明涉及从命名为rbl-1 [SEQ ID NO.1]的序列分离的寡核苷酸序列。 19]可以杀死或抑制微生物的生长，或阻止生成内源性表达的毒素。 通过使用筛选方法鉴定的这些ssDNA序列似乎可以作为必需生长功能的调节剂，其可以在三重生成，反义抑制水平或作为改变基因功能的适体的作用下起作用。 称为最小功能区域或MFR的序列尤其可用作治疗败血症的治疗剂和由诸如败血症和/或其中微生物是贡献剂的微生物引起的其它病症。