公开(公告)号：US20120100540A1

公开(公告)日：2012-04-26

申请号：US13278353

申请日：2011-10-21

Applicant: XINGYONG WU ,  Huei-Yu Fay WANG ,  Nan SU ,  Li-Chong WANG ,  Yuling LUO

Inventor： XINGYONG WU ,  Huei-Yu Fay WANG ,  Nan SU ,  Li-Chong WANG ,  Yuling LUO

IPC: C12Q1/68

CPC classification number: C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.

Abstract translation: 本发明公开了一种基于RNA方法和一般ISH信号扩增方法的组合来原位检测一种或多种靶核酸的方法。 这种新方法产生高信号强度，同时保持低信号放大的背景噪声。 结果可以一贯重现，该方法可以方便地用于常规诊所诊断。 此外，本发明涉及一种试剂盒，其包含用于灵敏检测一种或多种靶核酸的RNA检测和一般ISH信号扩增测定的组分。

公开(公告)号：US08658361B2

公开(公告)日：2014-02-25

申请号：US13278353

申请日：2011-10-21

Applicant: Xingyong Wu ,  Huei-Yu Fay Wang ,  Nan Su ,  Li-Chong Wang ,  Yuling Luo

Inventor： Xingyong Wu ,  Huei-Yu Fay Wang ,  Nan Su ,  Li-Chong Wang ,  Yuling Luo

IPC: C12Q1/68 ,  C12Q1/00 ,  G01N33/53 ,  C12Q1/28 ,  C12P19/34 ,  C12M1/34 ,  B01L3/00 ,  G01N31/22 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6841 ,  C12Q1/6818 ,  C12Q1/682 ,  C12Q2563/131 ,  C12Q2537/143 ,  C12Q2537/125

Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.

Abstract translation: 公开了一种基于原位杂交（ISH）测定方法和一般ISH信号扩增方法的组合原位检测一种或多种靶核酸的方法。 这种新方法产生高信号强度，同时保持低信号放大的背景噪声。 结果可以一贯重现，该方法可以方便地用于常规诊所诊断。 此外，本发明涉及一种试剂盒，其包含用于敏感检测一种或多种靶核酸的ISH测定和一般ISH信号扩增测定的组分。

公开(公告)号：US20120071343A1

公开(公告)日：2012-03-22

申请号：US13236889

申请日：2011-09-20

Applicant: XIAO-JUN MA ,  Xingyong Wu ,  Yuling Luo

Inventor： XIAO-JUN MA ,  Xingyong Wu ,  Yuling Luo

IPC: C40B30/04 ,  C12Q1/42 ,  C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF 15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.

Abstract translation: 本发明公开了一种用于诊断人类受试者中的黑素瘤的方法，以及用于对患有黑色素瘤复发风险的人类受试者提供预后的方法，以及用于确定人类受试者的黑色素瘤阶段的方法，包括 单独或与分泌的整联蛋白结合磷蛋白（SPP1）的表达水平相结合的磷酸酶和肌动蛋白调节物1（PHACTR1）基因或其片段的表达水平的测定步骤，优先表达黑素瘤（PRAME）抗原， ，生长分化因子15（GDF15）和趋化因子CXC基序配体10（CXCL10）基因。 此外，本发明涉及一种诊断试剂盒，其包含至少一种用于检测PHACTR1或其片段的表达的物质，或者与检测SPP1，PRAME，GDF15和CXCL10一起或与SPP1，PRAME，GDF15和CXCL10的检测结合用于诊断或预后 的黑色素瘤。