公开(公告)号：US20120121717A1

公开(公告)日：2012-05-17

申请号：US13142820

申请日：2008-12-30

申请人： Xu Chao ,  Yali Cui ,  Mingli Peng ,  Chao Chen ,  Xiaofang Xin ,  Ke Li ,  Wenli Hui

发明人： Xu Chao ,  Yali Cui ,  Mingli Peng ,  Chao Chen ,  Xiaofang Xin ,  Ke Li ,  Wenli Hui

IPC分类号： A61K9/14 ,  B05D3/00 ,  A61P31/00 ,  A61K31/704 ,  A61P35/00

CPC分类号： A61K9/1652 ,  A61K9/5094 ,  A61K31/704 ,  A61K49/183 ,  B82Y25/00 ,  H01F1/0054 ,  H01F1/061

摘要： The invention relates to Polysaccharide-coated GoldMag particles (DPGPs) and the method of its synthesis, which characterized GoldMag particles as a core and natural or synthetic biodegradable polysaccharide such as dextran, cyclodextrin and derivatives as shell. DPGPs are synthesized by mixing Polysaccharide-coated GoldMag particles (DPGPs) with drug through physical bond. The preparation of the drug-loaded composite particles include: preparing the polysaccharide-coated GoldMag particles and then loading the drug on the polysaccharide-coated GoldMag particles. The drug-loading process is carried out through directly mixing the polysaccharide-coated GoldMag particles with the drug solution by the shaker. That means the polysaccharide-coated GoldMag particles load the drug through affinity adsorption.

摘要翻译： 本发明涉及多糖包被的GoldMag颗粒（DPGP）及其合成方法，其特征在于以GoldMag颗粒为核心，以天然或合成的可生物降解多糖如葡聚糖，环糊精和衍生物为壳。 DPGPs通过物理粘合剂将多糖包被的GoldMag颗粒（DPGPs）与药物混合来合成。 负载药物的复合颗粒的制备包括：制备多糖包被的GoldMag颗粒，然后将药物装载在多糖包被的GoldMag颗粒上。 药物加载过程通过将振荡器与药物溶液直接混合，将多糖包被的GoldMag颗粒直接混合来进行。 这意味着多糖包被的GoldMag颗粒通过亲和吸附装载药物。

公开(公告)号：US08501459B2

公开(公告)日：2013-08-06

申请号：US12994017

申请日：2008-10-06

申请人： Chao Chen ,  Yitong Tang ,  Yali Cui ,  Juanli Zhu ,  Longlin Yu ,  Yiwen Gao ,  Zheng Li

发明人： Chao Chen ,  Yitong Tang ,  Yali Cui ,  Juanli Zhu ,  Longlin Yu ,  Yiwen Gao ,  Zheng Li

IPC分类号： C12M1/34 ,  C12M3/00 ,  C12Q1/68 ,  C12C19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6827 ,  C12Q2533/107 ,  C12Q2525/197

摘要： High-throughput detection for the interesting base or the mutation site in the nucleic acid sample can be achieved by means of the linear test probe pairs P1 and P2. The test probe pairs P1 and P2 respectively comprise either of the flanking complementary sequences which are adjacent to the interesting base or the mutation site in the nucleic acid sample. The invention can be applied to the re-sequencing the target nucleic acid sequence, the detection and analysis for the mutation, insertion, or deletion sites of a known nucleic acid sequence, and the genotyping of the pathogenic microorganism.

摘要翻译： 可以通过线性测试探针对P1和P2来实现核酸样品中有趣碱基或突变位点的高通量检测。 测试探针对P1和P2分别包含与感兴趣碱基或核酸样品中突变位点相邻的侧翼互补序列之一。 本发明可以应用于重新测序目标核酸序列，检测和分析已知核酸序列的突变，插入或缺失位点，以及病原微生物的基因分型。

公开(公告)号：US20110218115A1

公开(公告)日：2011-09-08

申请号：US12994017

申请日：2008-10-06

申请人： Chao Chen ,  Yitong Tang ,  Yali Cui ,  Juanli Zhu ,  Longlin Yu ,  Yiwen Gao ,  Zheng Li

发明人： Chao Chen ,  Yitong Tang ,  Yali Cui ,  Juanli Zhu ,  Longlin Yu ,  Yiwen Gao ,  Zheng Li

IPC分类号： C40B30/04 ,  C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2533/107 ,  C12Q2525/197

摘要： High-throughput detection for the interesting base or the mutation site in the nucleic acid sample can be achieved by the linear test probe pairs P1 and P2. The test probe pairs P1 and P2 respectively comprise either of the flanking complementary sequences which are adjacent to the interesting base or the mutation site in the nucleic acid sample. When the test probe pairs P1, P2 are annealed and hybridized to the nucleic acid sample, a gap will be generated at the interesting base or the mutation site position between the probe pairs and the sample. Divide the annealed hybrid sample into four equal reaction systems to which add dATP, dTTP, dCTP, dGTP, respectively. The test probe pairs P1 and P2 will be ligated into one single probe when adding the complementary nucleotide system under the DNA polymerase or ligase. After purified and amplified, the generated single probes are hybridized to the corresponding area in a common oligonucleotide microarray. The generated single probe will give a signal in the hybrid area, and therefore detect and analyze the hybrid signal to determine the base type or the mutation genotype at the detection position. The invention can be applied to the re-sequencing the target nucleic acid sequence, the detection and analysis for the mutation, insertion, or deletion sites of a known nucleic acid sequence, and the genotyping of the pathogenic microorganism.

摘要翻译： 可以通过线性测试探针对P1和P2来实现核酸样品中感兴趣碱基或突变位点的高通量检测。 测试探针对P1和P2分别包含与感兴趣碱基或核酸样品中突变位点相邻的侧翼互补序列之一。 当测试探针对P1，P2退火并与核酸样品杂交时，将在探针对和样品之间的感兴趣的碱基或突变位点位置产生间隙。 将退火的混合样品分成四个相等的反应体系，分别加入dATP，dTTP，dCTP，dGTP。 当在DNA聚合酶或连接酶下添加互补核苷酸系统时，测试探针对P1和P2将被连接到一个单个探针中。 纯化和扩增后，产生的单个探针与普通寡核苷酸微阵列中的相应区域杂交。 所产生的单个探针将在混合区域中产生信号，因此检测和分析混合信号以确定检测位置的基因型或突变基因型。 本发明可以应用于重新测序目标核酸序列，检测和分析已知核酸序列的突变，插入或缺失位点，以及病原微生物的基因分型。

公开(公告)号：US08409544B2

公开(公告)日：2013-04-02

申请号：US13142167

申请日：2008-12-31

申请人： Mingli Peng ,  Caiquan Zhang ,  Yali Cui ,  Chao Chen ,  Jinghui Zeng

发明人： Mingli Peng ,  Caiquan Zhang ,  Yali Cui ,  Chao Chen ,  Jinghui Zeng

IPC分类号： C01G49/02

CPC分类号： H01F1/0045 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/08 ,  C01P2004/32 ,  C01P2004/64 ,  C01P2006/42

摘要： Disclosed is a process of preparing magnetite nanoparticles, comprising the following steps: 1) preparing a ferric salt mixed system, wherein a soluble ferric salt is dissolved in glycol at ambient temperature, and then urea and polyethylene glycol are added and mixed homogeneously to obtain the trivalent iron salt mixed system, the mass ratio of glycol to the trivalent iron salt being 15:1 to 60:1, glycol to urea being 20:1 to 100:1, and glycol to polyethylene glycol being 20:1 to 100:1; 2) reacting, wherein the trivalent iron salt mixed system is transferred into a reaction autoclave, sealed and placed into a heating device to react at a temperature of 200 to 300° C. for 8 to 72 hours; and 3) washing, wherein after the reaction system is naturally cooled down to ambient temperature, the product is taken out, and washed with anhydrous ethanol and water in turn to obtain the magnetite nanoparticles. The soluble iron salt includes ferric chloride, ferric sulfate, ferric acetate and ferric nitrate. The obtained nanospheres exhibit a uniform distribution of the particle diameter with a good dispersity in water. The nanospheres have superparamagnetism, and their particle diameter can be controlled by varying the reaction time as desired.

摘要翻译： 公开了一种制备磁铁矿纳米颗粒的方法，包括以下步骤：1）制备铁盐混合体系，其中可溶性铁盐在环境温度下溶于二醇，然后加入尿素和聚乙二醇并均匀混合，得到 三价铁盐混合体系，二醇与三价铁盐的质量比为15:1至60:1，二醇与尿素的比例为20:1至100:1，二醇与聚乙二醇的比例为20:1至100:1 ; 2）反应，其中将三价铁盐混合体系转移到反应釜中，密封并置于加热装置中，在200-300℃的温度下反应8至72小时; 和3）洗涤，其中在反应体系自然冷却至环境温度后，取出产物，依次用无水乙醇和水洗涤，得到磁铁矿纳米颗粒。 可溶性铁盐包括氯化铁，硫酸铁，醋酸铁和硝酸铁。 得到的纳米球表现出均匀的粒径分布，在水中具有良好的分散性。 纳米球具有超顺磁性，并且可通过根据需要改变反应时间来控制它们的粒径。

公开(公告)号：US20110318261A1

公开(公告)日：2011-12-29

申请号：US13142167

申请日：2008-12-31

申请人： Mingli Peng ,  Caiquan Zhang ,  Yali Cui ,  Chao Chen ,  Jinghui Zeng

发明人： Mingli Peng ,  Caiquan Zhang ,  Yali Cui ,  Chao Chen ,  Jinghui Zeng

IPC分类号： C01G49/08 ,  B82Y40/00

CPC分类号： H01F1/0045 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/08 ,  C01P2004/32 ,  C01P2004/64 ,  C01P2006/42

摘要： Disclosed is a process of preparing magnetite nanoparticles, comprising the following steps: 1) preparing a ferric salt mixed system, wherein a soluble ferric salt is dissolved in glycol at ambient temperature, and then urea and polyethylene glycol are added and mixed homogeneously to obtain the trivalent iron salt mixed system, the mass ratio of glycol to the trivalent iron salt being 15:1 to 60:1, glycol to urea being 20:1 to 100:1, and glycol to polyethylene glycol being 20:1 to 100:1; 2) reacting, wherein the trivalent iron salt mixed system is transferred into a reaction autoclave, sealed and placed into a heating device to react at a temperature of 200 to 300° C. for 8 to 72 hours; and 3) washing, wherein after the reaction system is naturally cooled down to ambient temperature, the product is taken out, and washed with anhydrous ethanol and water in turn to obtain the magnetite nanoparticles. The soluble iron salt includes ferric chloride, ferric sulfate, ferric acetate and ferric nitrate. The obtained nanospheres exhibit a uniform distribution of the particle diameter with a good dispersity in water. The nanospheres have superparamagnetism, and their particle diameter can be controlled by varying the reaction time as desired.

摘要翻译： 公开了一种制备磁铁矿纳米颗粒的方法，包括以下步骤：1）制备铁盐混合体系，其中可溶性铁盐在环境温度下溶于二醇，然后加入尿素和聚乙二醇并均匀混合，得到 三价铁盐混合体系，二醇与三价铁盐的质量比为15:1至60:1，二醇与尿素的比例为20:1至100:1，二醇与聚乙二醇的比例为20:1至100:1 ; 2）反应，其中将三价铁盐混合体系转移到反应釜中，密封并置于加热装置中，在200-300℃的温度下反应8至72小时; 和3）洗涤，其中在反应体系自然冷却至环境温度后，取出产物，依次用无水乙醇和水洗涤，得到磁铁矿纳米颗粒。 可溶性铁盐包括氯化铁，硫酸铁，醋酸铁和硝酸铁。 得到的纳米球表现出均匀的粒径分布，在水中具有良好的分散性。 纳米球具有超顺磁性，并且可通过根据需要改变反应时间来控制它们的粒径。

公开(公告)号：US20120003321A1

公开(公告)日：2012-01-05

申请号：US13142159

申请日：2008-12-29

申请人： Mingli Peng ,  Yanhong Liu ,  Yali Cui ,  Chao Chen ,  Ke Li

发明人： Mingli Peng ,  Yanhong Liu ,  Yali Cui ,  Chao Chen ,  Ke Li

IPC分类号： A61K9/14 ,  A61K31/513 ,  A61K31/337 ,  A61K31/7068 ,  A61K31/437 ,  A61K31/4375 ,  A61K31/704 ,  A61K38/14 ,  B82Y5/00

CPC分类号： A61K9/0009 ,  A61K9/0019 ,  A61K9/1611 ,  A61K9/1652 ,  H01F1/083

摘要： The present invention relates to crosslinked dextran magnetic composite microparticles and a preparation process and a using method thereof. The composite microparticles comprise magnetic nanoparticles and dextran with crosslinked structure, wherein the magnetic nanoparticles are dispersed in the dextran with crosslinked structure. The process for preparing the composite microparticles comprises: preparing a dextran solution; synthesizing dextran magnetic composite microparticles; and synthesizing the crosslinked dextran magnetic composite microparticles. The using method of composite microparticles comprises: preparing crosslinked dextran magnetic composite microparticles loaded with anti-cancer drug; and adding a sustained-releasing solution thereto.

摘要翻译： 本发明涉及交联葡聚糖磁性复合微粒及其制备方法及其应用方法。 复合微粒包括具有交联结构的磁性纳米颗粒和葡聚糖，其中磁性纳米颗粒分散在具有交联结构的葡聚糖中。 制备复合微粒的方法包括：制备葡聚糖溶液; 合成葡聚糖磁复合微粒; 并合成交联的葡聚糖磁性复合微粒。 复合微粒的使用方法包括：制备负载抗癌药物的交联葡聚糖磁复合微粒; 并向其中加入缓释溶液。

公开(公告)号：US07175912B2

公开(公告)日：2007-02-13

申请号：US10833649

申请日：2004-04-28

申请人： Yali Cui ,  Chao Chen ,  Qiong Wang ,  Wenli Hui

发明人： Yali Cui ,  Chao Chen ,  Qiong Wang ,  Wenli Hui

IPC分类号： B32B5/16

CPC分类号： G01N33/5434 ,  Y10T428/12465 ,  Y10T428/2984 ,  Y10T428/2991

摘要： The invention relates to a super-paramagnetic composite particle with core/shell structure, preparation method and use thereof. The composite particle is consisted of a core portion and a shell portion coated on the surface of the core portion, wherein said core portion is 10–70% by weight and said shell portion is 30–90% by weight based on the total weight of the composite particle, and said core portion is consisted of magnetic particles of Fe3O4, γ-Fe2O3 or other ferric oxides, or magnetic particles of ferrites of tervalent ferrum and bivalent manganese, nickel, zinc or copper, and the said shell portion is consisted of elementary gold or silver. The particle has an average diameter of 0.05–50 μm. The preparation method comprises preparing the core portion magnetic particle by chemical co-precipitation and depositing gold or silver to coat the magnetic particle by chemical reduction. The composite particle can label biological materials or nonbiological materials selected from the group consisted of nucleic acid, antigen, antibody, enzyme, polypeptide, polysaccharide, avidin, streptavidin or cell and the like, and be used in biological test and nonbiological test.

摘要翻译： 本发明涉及具有核/壳结构的超顺磁复合颗粒，其制备方法和用途。 复合颗粒由核心部分和涂覆在芯部分表面上的壳部分组成，其中所述芯部分为10-70重量％，所述壳部分为基于总重量的30-90重量％ 复合颗粒和所述芯部分由Fe 3 O 4 O 3，γ-Fe 2 O 3 3的磁性颗粒组成， 或其它三价铁氧化物，或铁酸盐和二价锰，镍，锌或铜的铁氧体的磁性颗粒，并且所述壳部分由基本金或银组成。 颗粒的平均直径为0.05-50μm。 制备方法包括通过化学共沉淀制备核心部分磁性颗粒，并通过化学还原沉积金或银来涂覆磁性颗粒。 复合颗粒可以标记选自核酸，抗原，抗体，酶，多肽，多糖，抗生物素蛋白，链霉抗生物素蛋白或细胞等的生物材料或非生物材料，并用于生物试验和非生物试验。