公开(公告)号：US20050202572A1

公开(公告)日：2005-09-15

申请号：US11006757

申请日：2004-12-08

申请人： Yasuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

发明人： Yasuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

IPC分类号： G01N33/53 ,  G01N27/72 ,  G01N33/542 ,  G01N33/553

CPC分类号： G01N33/54373 ,  G01N27/745

摘要： The present invention provides an immunoassay technique which enables efficient detection of antigen-antibody reaction with high sensitivity by a magnetic method using magnetic particles and a SQUID magnetic sensor or sensors. A system based on the technique includes a disk-shaped sample holder which holds on a circle a plurality of sample containers for accommodating marked samples, resulting from marking of samples with magnetic particles by antigen-antibody reaction; rotating means for rotating the holder around its central shaft; magnetizing means for magnetizing the marked samples outside a magnetic shield; and a magnetic sensor for detecting, within the magnetic shield, magnetic fields generated from the marked samples which have been magnetized. By rotation of the holder, areas fixing and holding different ones of the sample containers are successively inserted into the magnetic shield, and the magnetization of the marked samples accommodated in first ones of the sample containers and the detection of magnetic fields generated from the marked samples accommodated in second ones of the sample containers are executed in parallel.

摘要翻译： 本发明提供了一种免疫测定技术，其能够通过使用磁性颗粒和SQUID磁性传感器的磁性方法高灵敏度地高效检测抗原 - 抗体反应。 基于该技术的系统包括：盘形样品保持器，其保持圆圈上的多个样品容器，用于容纳标记的样品，通过抗原 - 抗体反应用磁性颗粒标记样品; 用于使支架围绕其中心轴旋转的旋转装置; 用于将标记样品磁化在磁屏蔽外的磁化装置; 以及磁传感器，用于在磁屏蔽内检测从已经被磁化的标记样品产生的磁场。 通过保持器的旋转，将固定和保持不同样本容器的区域依次插入到磁屏蔽中，并且标记的样品的磁化容纳在第一个样品容器中，并且检测从标记的样品产生的磁场 容纳在第二个样品容器中并行执行。