公开(公告)号：US20050202572A1

公开(公告)日：2005-09-15

申请号：US11006757

申请日：2004-12-08

申请人： Yasuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

发明人： Yasuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

IPC分类号： G01N33/53 ,  G01N27/72 ,  G01N33/542 ,  G01N33/553

CPC分类号： G01N33/54373 ,  G01N27/745

摘要： The present invention provides an immunoassay technique which enables efficient detection of antigen-antibody reaction with high sensitivity by a magnetic method using magnetic particles and a SQUID magnetic sensor or sensors. A system based on the technique includes a disk-shaped sample holder which holds on a circle a plurality of sample containers for accommodating marked samples, resulting from marking of samples with magnetic particles by antigen-antibody reaction; rotating means for rotating the holder around its central shaft; magnetizing means for magnetizing the marked samples outside a magnetic shield; and a magnetic sensor for detecting, within the magnetic shield, magnetic fields generated from the marked samples which have been magnetized. By rotation of the holder, areas fixing and holding different ones of the sample containers are successively inserted into the magnetic shield, and the magnetization of the marked samples accommodated in first ones of the sample containers and the detection of magnetic fields generated from the marked samples accommodated in second ones of the sample containers are executed in parallel.

摘要翻译： 本发明提供了一种免疫测定技术，其能够通过使用磁性颗粒和SQUID磁性传感器的磁性方法高灵敏度地高效检测抗原 - 抗体反应。 基于该技术的系统包括：盘形样品保持器，其保持圆圈上的多个样品容器，用于容纳标记的样品，通过抗原 - 抗体反应用磁性颗粒标记样品; 用于使支架围绕其中心轴旋转的旋转装置; 用于将标记样品磁化在磁屏蔽外的磁化装置; 以及磁传感器，用于在磁屏蔽内检测从已经被磁化的标记样品产生的磁场。 通过保持器的旋转，将固定和保持不同样本容器的区域依次插入到磁屏蔽中，并且标记的样品的磁化容纳在第一个样品容器中，并且检测从标记的样品产生的磁场 容纳在第二个样品容器中并行执行。

公开(公告)号：US07993581B2

公开(公告)日：2011-08-09

申请号：US11006757

申请日：2004-12-08

申请人： Yusuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

发明人： Yusuke Seki ,  Akira Tsukamoto ,  Daisuke Suzuki ,  Masahiro Yamaoka ,  Nami Sugita ,  Akihiko Kandori ,  Kazuo Saitoh

IPC分类号： G01N1/36 ,  G01N33/553

CPC分类号： G01N33/54373 ,  G01N27/745

摘要： The present invention provides an immunoassay technique which enables efficient detection of antigen-antibody reaction with high sensitivity by a magnetic method using magnetic particles and a SQUID magnetic sensor or sensors. A system based on the technique includes a disk-shaped sample holder which holds on a circle a plurality of sample containers for accommodating marked samples, resulting from marking of samples with magnetic particles by antigen-antibody reaction; rotating means for rotating the holder around its central shaft; magnetizing means for magnetizing the marked samples outside a magnetic shield; and a magnetic sensor for detecting, within the magnetic shield, magnetic fields generated from the marked samples which have been magnetized. By rotation of the holder, areas fixing and holding different ones of the sample containers are successively inserted into the magnetic shield, and the magnetization of the marked samples accommodated in first ones of the sample containers and the detection of magnetic fields generated from the marked samples accommodated in second ones of the sample containers are executed in parallel.

摘要翻译： 本发明提供了一种免疫测定技术，其能够通过使用磁性颗粒和SQUID磁性传感器的磁性方法高灵敏度地高效检测抗原 - 抗体反应。 基于该技术的系统包括：盘形样品保持器，其保持圆圈上的多个样品容器，用于容纳标记的样品，通过抗原 - 抗体反应用磁性颗粒标记样品; 用于使支架围绕其中心轴旋转的旋转装置; 用于将标记样品磁化在磁屏蔽外的磁化装置; 以及磁传感器，用于在磁屏蔽内检测从已经被磁化的标记样品产生的磁场。 通过保持器的旋转，将固定和保持不同样本容器的区域依次插入到磁屏蔽中，并且标记的样品的磁化容纳在第一个样品容器中，并且检测从标记的样品产生的磁场 容纳在第二个样品容器中并行执行。

公开(公告)号：US20080284413A1

公开(公告)日：2008-11-20

申请号：US12149990

申请日：2008-05-12

申请人： Akira Tsukamoto ,  Daisuke Suzuki ,  Akihiko Kandori

发明人： Akira Tsukamoto ,  Daisuke Suzuki ,  Akihiko Kandori

IPC分类号： G01N27/74

CPC分类号： G01N33/54333

摘要： Accurate measurement cannot be performed due to magnetic signals from magnetic impurities included in a specimen container. By finding a difference between two measurement signals obtained by applying an external magnetic field for orientation to respective normal and reverse directions, the magnetic signals from the magnetic impurities included in the specimen container which are not dependent on the external magnetic field for orientation can be cancelled. The influence of the magnetic impurities included in the specimen container is reduced, and the signal of the intended bound magnetic marker can be measured with high sensitivity.

摘要翻译： 由于样品容器中包含的磁性杂质的磁信号，无法进行准确的测量。 通过在相应的正反方向上施加用于取向的外部磁场获得的两个测量信号之间的差异，可以取消不依赖于用于取向的外部磁场的样本容器中包含的磁性杂质的磁信号 。 包含在标本容器中的磁性杂质的影响减小，可以高灵敏度地测量预期的结合磁性标记的信号。

公开(公告)号：US08193003B2

公开(公告)日：2012-06-05

申请号：US12149990

申请日：2008-05-12

申请人： Akira Tsukamoto ,  Daisuke Suzuki ,  Akihiko Kandori

发明人： Akira Tsukamoto ,  Daisuke Suzuki ,  Akihiko Kandori

IPC分类号： G01N33/553 ,  G01N33/53

CPC分类号： G01N33/54333

摘要： Accurate measurement cannot be performed due to magnetic signals from magnetic impurities included in a specimen container. By finding a difference between two measurement signals obtained by applying an external magnetic field for orientation to respective normal and reverse directions, the magnetic signals from the magnetic impurities included in the specimen container which are not dependent on the external magnetic field for orientation can be cancelled. The influence of the magnetic impurities included in the specimen container is reduced, and the signal of the intended bound magnetic marker can be measured with high sensitivity.

摘要翻译： 由于样品容器中包含的磁性杂质的磁信号，无法进行准确的测量。 通过在相应的正反方向上施加用于取向的外部磁场获得的两个测量信号之间的差异，可以取消不依赖于用于取向的外部磁场的样本容器中包含的磁性杂质的磁信号 。 包含在标本容器中的磁性杂质的影响减小，可以高灵敏度地测量预期的结合磁性标记的信号。

公开(公告)号：US06853185B2

公开(公告)日：2005-02-08

申请号：US10844480

申请日：2004-05-13

申请人： Akira Tsukamoto ,  Koichi Yokosawa ,  Daisuke Suzuki ,  Akihiko Kandori ,  Keiji Tsukada

发明人： Akira Tsukamoto ,  Koichi Yokosawa ,  Daisuke Suzuki ,  Akihiko Kandori ,  Keiji Tsukada

IPC分类号： A61B5/05 ,  G01R33/025 ,  G01R33/035 ,  G01R33/02

CPC分类号： G01R33/0358 ,  G01R33/025

摘要： A magnetic field measurement system for canceling an external field is provided, in which plurality of sensing magnetometers (3) for measuring a magnetic field signal in a direction perpendicular to the center axis of a cylindrical magnetic shield (1) are arranged in two dimensions on a plane parallel to the center axis and a reference magnetometer (4) for measuring the external field parallel to the center axis as a reference signal is disposed on a plane perpendicular to the plane parallel to the center axis. The reference signal multiplied by a specified factor is subtracted from a difference between signals from the adjacent sensing magnetometers (3). The magnetic field measurement system allows measurement of an extremely weak magnetic field by efficiently canceling the external field.

摘要翻译： 提供了用于消除外部场的磁场测量系统，其中用于在与圆柱形磁屏蔽（1）的中心轴垂直的方向上测量磁场信号的多个感测磁力计（3）被二维地布置在 平行于中心轴的平面和用于测量与中心轴平行的外场作为参考信号的参考磁力计（4）设置在垂直于平行于中心轴的平面的平面上。 从相邻感测磁力计（3）的信号之间的差中减去乘以指定因子的参考信号。 磁场测量系统允许通过有效地消除外部场来测量极弱的磁场。

公开(公告)号：US06815949B2

公开(公告)日：2004-11-09

申请号：US10162748

申请日：2002-06-06

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Koichi Yokosawa ,  Daisuke Suzuki ,  Akira Tsukamoto

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Keiji Tsukada ,  Koichi Yokosawa ,  Daisuke Suzuki ,  Akira Tsukamoto

IPC分类号： G01R33035

CPC分类号： G01R33/0356 ,  A61B5/04005 ,  A61B5/04007 ,  A61B5/04008 ,  A61B5/0522

摘要： An apparatus can detect a magnetic field with a high sensitivity using an ordinary-temperature pickup coil even when the pickup coil is arranged outside a cryostat. Specifically, the apparatus for measuring a magnetic field includes a pickup coil for detecting an external magnetic field, a SQUID electrically or magnetically connected to the pickup coil, a cryostat for holding the SQUID at low temperatures, and a driving device for driving the SQUID. The pickup coil is made of a normal-conducting material and is placed at an ordinary temperature outside the cryostat. By arranging outside the cryostat, the pickup coil can be brought close to a subject and can thereby detect a weak magnetic field in the subject with a high sensitivity.

公开(公告)号：US20130121879A1

公开(公告)日：2013-05-16

申请号：US13810832

申请日：2011-07-19

申请人： Ryuzo Kawabata ,  Takako Mizoguchi ,  Akira Tsukamoto ,  Akihiko Kandori ,  Tomoko Yoshimura

发明人： Ryuzo Kawabata ,  Takako Mizoguchi ,  Akira Tsukamoto ,  Akihiko Kandori ,  Tomoko Yoshimura

IPC分类号： G01N33/543

CPC分类号： G01N33/54333 ,  G01N27/745

摘要： The disclosed magnetic immunoassay device, which performs magnetic immunoassays using antigen-antibody reactions, can perform speedy immunoassays without bound/free separation in the test samples. The device is also practical, being capable of stable magnetism measurement without magnetic shielding. The disclosed magnetic immunoassay device is provided with: an excitation coil that uses an AC magnetic field to magnetize a test sample containing a magnetic marker; a magnetism sensor that measures magnetism in the test sample and outputs a magnetism signal; and a displacement sensor for detecting changes in the distance between the test sample and the magnetism sensor. By optimally setting the bandwidth of a lock-in amplifier, which detects changes in the phase of the magnetism signal outputted by the magnetism sensor, and the rotational speed produced by a drive system, which moves the test sample at low speeds, the impact of environment magnetic noise is reduced, and correcting the magnetism signal using distance information obtained from the displacement sensor allows stable magnetism measurement.

摘要翻译： 所公开的使用抗原 - 抗体反应进行磁性免疫测定的磁性免疫测定装置可以在测试样品中进行快速的免疫测定而无限制/自由分离。 该装置也是实用的，能够在没有磁屏蔽的情况下进行稳定的磁测量。 所公开的磁免疫测定装置具有：励磁线圈，其使用交流磁场来磁化含有磁性标记的测试样品; 磁传感器，其测量测试样品中的磁性并输出磁信号; 以及用于检测测试样本和磁传感器之间的距离变化的位移传感器。 通过最佳地设置检测由磁传感器输出的磁信号的相位变化的锁定放大器的带宽和由低速移动测试样本的驱动系统产生的转速， 环境磁噪声降低，并且使用从位移传感器获得的距离信息来校正磁信号，可以进行稳定的磁测量。

公开(公告)号：US20080306373A1

公开(公告)日：2008-12-11

申请号：US12155191

申请日：2008-05-30

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Daisuke Suzuki

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Daisuke Suzuki

IPC分类号： A61B7/00 ,  A61B5/055

CPC分类号： A61B5/1126 ,  A61B5/4205 ,  A61B5/6822 ,  A61B7/008

摘要： Disclosed is a swallowing test apparatus including a laryngeal part displacement detection apparatus for detecting a distance of two positions located laterally at a laryngeal part of a subject, a swallowing sound detection apparatus for detecting a swallowing sound of the patient during swallowing, a display, a processing apparatus for displaying a waveform representing the distance of the two positions at the laryngeal part generated based on a signal obtained from the laryngeal part displacement detection apparatus, and a waveform representing the swallowing sound generated based on a signal obtained from the swallowing sound detection apparatus on the display.

摘要翻译： 本发明公开了一种吞咽试验装置，其包括：喉部位移检测装置，用于检测横向位于被检体的喉部的两个位置的距离;吞咽声检测装置，用于在吞咽期间检测患者的吞咽声，显示器， 处理装置，用于根据从喉部位移检测装置获得的信号显示表示在喉部产生的两个位置的距离的波形，以及表示基于从吞咽声音检测装置获得的信号产生的吞咽声音的波形 在显示器上。

公开(公告)号：US06681131B2

公开(公告)日：2004-01-20

申请号：US09977213

申请日：2001-10-16

申请人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Daisuke Suzuki ,  Koichi Yokosawa ,  Keiji Tsukada

发明人： Akihiko Kandori ,  Tsuyoshi Miyashita ,  Daisuke Suzuki ,  Koichi Yokosawa ,  Keiji Tsukada

IPC分类号： A61B505

CPC分类号： A61B5/04007 ,  A61B5/7239 ,  A61B5/7285

摘要： An apparatus for measuring bio-magnetic fields includes a plurality of magnetometers for detecting magnetic fields generated from a live body; a driving circuit for driving the magnetometers; a computer for collecting output signals of the driving circuit in the form of data representing at least one waveform of the magnetic fields generated from the live body and for performing an arithmetic processing on the data representing the waveform of magnetic fields, a display unit, and at least one signal processing circuit for processing output signals of the driving circuit. The computer further performs an arithmetic processing of first-order differentiation on the waveform of the magnetic fields in a z direction with respect to time in order to create an arrow map and a contour map. The apparatus can also detect mechanical movement which occurs as blood flows in organs and organs move.

摘要翻译： 一种用于测量生物磁场的装置包括：用于检测从活体产生的磁场的多个磁力计; 用于驱动磁力计的驱动电路; 用于以表示从活体产生的磁场的至少一个波形的数据形式收集驱动电路的输出信号并且对表示磁场波形的数据执行算术处理的计算机，显示单元和 至少一个信号处理电路，用于处理驱动电路的输出信号。 计算机进一步对z方向上的磁场波形进行一阶微分的运算处理，以便产生箭头图和轮廓图。 该装置还可以检测器官和器官中的血液流动时发生的机械运动。

公开(公告)号：US06528994B2

公开(公告)日：2003-03-04

申请号：US09941752

申请日：2001-08-30

申请人： Daisuke Suzuki ,  Atsushi Ninomiya ,  Tsuyoshi Miyashita ,  Akihiko Kandori ,  Keiji Tsukada ,  Koichi Yokosawa

发明人： Daisuke Suzuki ,  Atsushi Ninomiya ,  Tsuyoshi Miyashita ,  Akihiko Kandori ,  Keiji Tsukada ,  Koichi Yokosawa

IPC分类号： G01R3302

CPC分类号： G01R33/24

摘要： A magnetic field measuring apparatus has a magnetic field shielding apparatus including non-magnetic cylindrical members on which high-permeability sheets are located in a state of being partially overlapped with each other. The cylindrical members are located to surround an axis of a first direction concentrically and having a hollow portion, the cylindrical member located on an innermost-side having a first opening at one end of the first direction, a second opening at the other end of the first direction, and a third opening penetrating the cylindrical members in a direction perpendicular to the axis of the first direction. In addition, the magnetic field measuring apparatus has a living-body holder located in an inner-side space of the cylindrical member and a cryostat inserted into the third opening and maintaining SQUID magnetic-flux meters.