公开(公告)号：US20100168028A1

公开(公告)日：2010-07-01

申请号：US12718997

申请日：2010-03-07

申请人： HARN-JING TERNG ,  YI-JEN LEE ,  WOAN-JEN LEE

发明人： HARN-JING TERNG ,  YI-JEN LEE ,  WOAN-JEN LEE

IPC分类号： A61K38/16 ,  C07K14/00 ,  C12P21/00 ,  A61P35/00 ,  A61P35/04

CPC分类号： C07K14/4702

摘要： Active fragments of hcrmp1 that are capable of inhibiting cell proliferation, invasive activity, and metastasis of cancer and methods of using the same for treating cancer are disclosed. The method can also be used prior to, or in combination with, the administration a chemotherapy agent. Vectors capable of expressing an hcrmp1, variants of hcrmp1, fragments of hcrmp1 and/or variants of fragments of hcrmp1 are also disclosed.

摘要翻译： 公开了能够抑制癌症的细胞增殖，侵袭活性和转移的hcrmp1的活性片段及其用于治疗癌症的方法。 该方法也可以在施用化疗剂之前或与其组合使用。 还公开了能够表达hcrmp1，hcrmp1的变体，hcrmp1的片段和/或hcrmp1片段的变体的载体。

公开(公告)号：US20080287361A1

公开(公告)日：2008-11-20

申请号：US12076722

申请日：2008-03-21

申请人： Harn-Jing Terng ,  Yi-Jen Lee ,  Woan-Jen Lee

发明人： Harn-Jing Terng ,  Yi-Jen Lee ,  Woan-Jen Lee

IPC分类号： A61K38/00 ,  C07K14/00 ,  C12N15/00 ,  A61P31/00 ,  C12N15/11

CPC分类号： C07K14/4702

摘要： The present invention discloses a method for treating cancer by using hCRMP1 and/or active fragments thereof, as well as the active fragments of hCRMP1 that are capable of inhibiting cell proliferation, invasive activity, and metastasis of cancer. The method can also be used prior to, or in combination with, the administration a chemotherapy agent. A vector capable of expressing an hCRMP1, a variant of hCRMP1, a fragment of hCRMP1 or a variant of a fragment of hCRMP1 is also disclosed.

摘要翻译： 本发明公开了一种通过使用hCRMP1和/或其活性片段以及能够抑制癌细胞增殖，侵袭活性和转移的hCRMP1的活性片段来治疗癌症的方法。 该方法也可以在施用化疗剂之前或与其组合使用。 还公开了能够表达hCRMP1的载体，hCRMP1的变体，hCRMP1的片段或hCRMP1的片段的变体。

公开(公告)号：US20050182016A1

公开(公告)日：2005-08-18

申请号：US11051328

申请日：2005-02-07

申请人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

发明人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

IPC分类号： A61K48/00 ,  C12N5/08 ,  C12N15/85 ,  C12P33/10

CPC分类号： C12N15/85 ,  A61K48/00 ,  C12N2830/00

摘要： This invention provides a transcription unit which is isolated from the upstream nucleic acid sequence of the collapsing response mediator protein-1 (CRMP-1) gene, an invasion-suppressor gene. The transcription unit contains a nucleic acid regulatory sequence which demonstrates promoter and/or regulatory activities (such as providing a transcription factor binding site) to enhance the expression of the CRMP-1 and/or a reporter protein. The invention also provides a DNA construct containing this transcription unit which can be transfected into a host cell. Additionally, the invention provides methods to enhance the expression of CRMP-1 and/or the reporter protein. The over-expression of CRMP-1 in a cancer cell can inhibit the metastasis of the cancer cell.

摘要翻译： 本发明提供了从塌陷反应介质蛋白-1（CRMP-1）基因的上游核酸序列，侵袭抑制基因中分离的转录单位。 转录单元含有证明启动子和/或调节活性（例如提供转录因子结合位点）以增强CRMP-1和/或报道蛋白表达的核酸调节序列。 本发明还提供含有该转录单位的DNA构建体，其可转染入宿主细胞。 另外，本发明提供了增强CRMP-1和/或报道蛋白表达的方法。 癌细胞中CRMP-1的过度表达可以抑制癌细胞的转移。

公开(公告)号：US07655779B2

公开(公告)日：2010-02-02

申请号：US11785701

申请日：2007-04-19

申请人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy J. W. Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

发明人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy J. W. Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

IPC分类号： C07H21/02

CPC分类号： C12N15/85 ,  A61K48/00 ,  C12N2830/00

摘要： This invention provides a transcription unit which is isolated from the upstream nucleic acid sequence of the collapsing response mediator protein-1 (CRMP-1) gene, an invasion-suppressor gene. The transcription unit contains a nucleic acid regulatory sequence which demonstrates promoter and/or regulatory activities (such as providing a transcription factor binding site) to enhance the expression of the CRMP-1 and/or a reporter protein. The invention also provides a DNA construct containing this transcription unit which can be transfected into a host cell. Additionally, the invention provides methods to enhance the expression of CRMP-1 and/or the reporter protein. The over-expression of CRMP-1 in a cancer cell can inhibit the metastasis of the cancer cell.

摘要翻译： 本发明提供了从塌陷反应介质蛋白-1（CRMP-1）基因的上游核酸序列，侵袭抑制基因中分离的转录单位。 转录单元含有证明启动子和/或调节活性（例如提供转录因子结合位点）以增强CRMP-1和/或报道蛋白表达的核酸调节序列。 本发明还提供含有该转录单位的DNA构建体，其可转染入宿主细胞。 另外，本发明提供了增强CRMP-1和/或报道蛋白表达的方法。 癌细胞中CRMP-1的过度表达可以抑制癌细胞的转移。

公开(公告)号：US07211660B2

公开(公告)日：2007-05-01

申请号：US11051328

申请日：2005-02-07

申请人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy J. W. Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

发明人： Pan-Chyr Yang ,  Tse-Ming Hong ,  Jeremy J. W. Chen ,  Cheng-Chung Wu ,  Jin-Yuan Shin ,  Yi-Jen Lee

IPC分类号： C07H21/04 ,  C12P21/04 ,  C12N15/86

CPC分类号： C12N15/85 ,  A61K48/00 ,  C12N2830/00

摘要： This invention provides a transcription unit which is isolated from the upstream nucleic acid sequence of the collapsing response mediator protein-1 (CRMP-1) gene, an invasion-suppressor gene. The transcription unit contains a nucleic acid regulatory sequence which demonstrates promoter and/or regulatory activities (such as providing a transcription factor binding site) to enhance the expression of the CRMP-1 and/or a reporter protein. The invention also provides a DNA construct containing this transcription unit which can be transfected into a host cell. Additionally, the invention provides methods to enhance the expression of CRMP-1 and/or the reporter protein. The over-expression of CRMP-1 in a cancer cell can inhibit the metastasis of the cancer cell.

摘要翻译： 本发明提供了从塌陷反应介质蛋白-1（CRMP-1）基因的上游核酸序列，侵袭抑制基因中分离的转录单位。 转录单元含有证明启动子和/或调节活性（例如提供转录因子结合位点）以增强CRMP-1和/或报道蛋白表达的核酸调节序列。 本发明还提供含有该转录单位的DNA构建体，其可转染入宿主细胞。 另外，本发明提供了增强CRMP-1和/或报道蛋白表达的方法。 癌细胞中CRMP-1的过度表达可以抑制癌细胞的转移。

公开(公告)号：US07741464B2

公开(公告)日：2010-06-22

申请号：US12076722

申请日：2008-03-21

申请人： Harn-Jing Terng ,  Yi-Jen Lee ,  Woan-Jen Lee

发明人： Harn-Jing Terng ,  Yi-Jen Lee ,  Woan-Jen Lee

IPC分类号： C07H21/02

CPC分类号： C07K14/4702

摘要： The present invention discloses a method for treating cancer by using hCRMP1 and/or active fragments thereof, as well as the active fragments of hCRMP1 that are capable of inhibiting cell proliferation, invasive activity, and metastasis of cancer. The method can also be used prior to, or in combination with, the administration a chemotherapy agent. A vector capable of expressing an hCRMP1, a variant of hCRMP1, a fragment of hCRMP1 or a variant of a fragment of hCRMP1 is also disclosed.

摘要翻译： 本发明公开了一种通过使用hCRMP1和/或其活性片段以及能够抑制癌细胞增殖，侵袭活性和转移的hCRMP1的活性片段来治疗癌症的方法。 该方法也可以在施用化疗剂之前或与其组合使用。 还公开了能够表达hCRMP1的载体，hCRMP1的变体，hCRMP1的片段或hCRMP1的片段的变体。