Linoleic Acid Isomerase and its Application in Production of Conjugated Linoleic Acid

    公开(公告)号:US20220017886A1

    公开(公告)日:2022-01-20

    申请号:US17497963

    申请日:2021-10-10

    IPC分类号: C12N9/90 C12P7/64

    摘要: Disclosed is linoleic acid isomerases and their application in production of conjugated linoleic acid, which belongs to the technical fields of protein engineering and microbial engineering. The linoleic acid isomerase derived from Bifidobacterium is used to produce the conjugated linoleic acid. The recombinant E. coli containing the linoleic acid isomerase of the invention is added into a reaction system containing linoleic acid and react for 3 h to produce conjugated linoleic acids. The conversion rate of the conjugated linoleic acid of the invented method ranges from 12.1% to 42.1%, and the percentage of cis9, trans11-CLA in the conjugated linoleic acid can reach 84.3% to 89.1%. The invention provides a method for using microorganisms to produce conjugated linoleic acids with high safety and yield where cis9, trans11-CLA isomer is the major form in the conjugated linoleic acid products.

    Diacylglycerol Acyltransferase 1 and Application Thereof in Production of Triacylglycerol

    公开(公告)号:US20210403884A1

    公开(公告)日:2021-12-30

    申请号:US17469899

    申请日:2021-09-09

    IPC分类号: C12N9/10 C12P7/64

    摘要: Disclosed is a diacylglycerol acyltransferase 1, a recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1, and application thereof in production of triacylglycerol. The diacylglycerol acyltransferase 1 of the invention has a function of catalyzing synthesis of triacylglycerol. After the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 of the invention is subjected to induction culture for 48 h, the content of total fatty acid and triacylglycerol in the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 can be respectively increased by 1.94 folds and 12.09 folds as compared with those of Saccharomyces cerevisiae without the recombinant diacylglycerol acyltransferase 1. The instant invention provides a method for improving the ability of microorganisms to produce polyunsaturated fatty acids (PUFAs) by means of genetic engineering.

    VEHICLE SAFETY ENHANCEMENT SYSTEMS AND METHODS
    9.
    发明申请
    VEHICLE SAFETY ENHANCEMENT SYSTEMS AND METHODS 有权
    车辆安全增强系统和方法

    公开(公告)号:US20120158249A1

    公开(公告)日:2012-06-21

    申请号:US13314188

    申请日:2011-12-08

    IPC分类号: B60R21/013

    CPC分类号: B60R21/0132 B60R21/0136

    摘要: A system for vehicle safety enhancement is disclosed. The system comprises a sudden slowdown detection subsystem, a slight bump detection subsystem, an airbag activation and brake malfunction detection subsystem, and a rollover detection subsystem. A controlling unit is connected to each subsystem for determining a severity level of a collision involving a vehicle, and the controlling unit is in turn connected to a communication unit for sending an alert signal based on the severity level. A method for vehicle safety enhancement is also disclosed.

    摘要翻译: 公开了一种用于车辆安全性增强的系统。 该系统包括突然减速检测子系统,轻微碰撞检测子系统,气囊激活和制动故障检测子系统以及翻转检测子系统。 控制单元连接到每个子系统以确定涉及车辆的碰撞的严重性级别,并且控制单元又连接到通信单元,用于基于严重性级别发送警报信号。 还公开了一种用于车辆安全性增强的方法。