公开(公告)号：US20090269802A1

公开(公告)日：2009-10-29

申请号：US11852609

申请日：2007-09-10

申请人： Yoshinao KOJIMA ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

发明人： Yoshinao KOJIMA ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

IPC分类号： C12P21/02 ,  C12N9/10 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12P19/18

CPC分类号： C12N9/1051 ,  C12P19/58

摘要： The object of the present invention is to provide α1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme.What is provided includes the following polypeptides (a) and (b), and DNAs encoding thereof: (a) a polypeptide consisting of an amino acid sequence represented by the amino acid Nos. 46-353 in SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a galactose residue from a galactose donor to C4 position of galactose residue of lactosylceramide or galactosylceramide which serves as an acceptor.

摘要翻译： 本发明的目的是提供α1,4-半乳糖基转移酶以将半乳糖残基转移到乳糖基神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位和编码该酶的DNA。 提供的包括以下多肽（a）和（b）及其编码的DNA：（a）由SEQ ID NO：2中的氨基酸编号46-353表示的氨基酸序列组成的多肽; 或（b）包含在（a）的氨基酸序列中包含一个或几个氨基酸的取代，缺失，插入或转座的氨基酸序列的多肽，并且具有从半乳糖供体转移半乳糖残基的酶活性 至作为受体的乳糖神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位置。

公开(公告)号：US20050019867A1

公开(公告)日：2005-01-27

申请号：US10895131

申请日：2004-07-21

申请人： Yoshinao Kojima ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

发明人： Yoshinao Kojima ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C12P19/58 ,  C12R1/91 ,  C07H21/04 ,  C08B37/00 ,  C12P19/04

CPC分类号： C12N9/1051 ,  C12P19/58

摘要： The object of the present invention is to provide α1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme. What is provided includes the following polypeptides (a) and (b), and DNAs encoding thereof: (a) a polypeptide consisting of an amino acid sequence represented by the amino acid Nos. 46-353 in SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a galactose residue from a galactose donor to C4 position of galactose residue of lactosylceramide or galactosylceramide which serves as an acceptor.

摘要翻译： 本发明的目的是提供α1,4-半乳糖基转移酶以将半乳糖残基转移到乳糖基神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位和编码该酶的DNA。 提供的包括以下多肽（a）和（b）及其编码的DNA：（a）由SEQ ID NO：2中的氨基酸编号46-353表示的氨基酸序列组成的多肽; 或（b）包含在（a）的氨基酸序列中包含一个或几个氨基酸的取代，缺失，插入或转座的氨基酸序列的多肽，并且具有从半乳糖供体转移半乳糖残基的酶活性 至作为受体的乳糖神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位置。

公开(公告)号：US06783966B1

公开(公告)日：2004-08-31

申请号：US09641701

申请日：2000-08-21

申请人： Yoshinao Kojima ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

发明人： Yoshinao Kojima ,  Satoshi Fukumoto ,  Keiko Furukawa ,  Tetsuya Okajima ,  Koichi Furukawa

IPC分类号： C12N1554

CPC分类号： C12N9/1051 ,  C12P19/58

摘要： The object of the present invention is to provide &agr;1,4-galactosyltransferase to transfer a galactose residue to C4 position of galactose residue of lactosylceramide or galactosylceramide, and DNA coding for the enzyme. What is provided includes the following polypeptides (a) and (b), and DNAs encoding thereof: (a) a polypeptide consisting of an amino acid sequence represented by the amino acid Nos. 46-353 in SEQ ID NO: 2; or (b) a polypeptide which comprises an amino acid sequence including substitution, deletion, insertion or transposition of one or few amino acids in the amino acid sequence of (a) and which has an enzymatic activity to transfer a galactose residue from a galactose donor to C4 position of galactose residue of lactosylceramide or galactosylceramide which serves as an acceptor.

摘要翻译： 本发明的目的是提供α1,4-半乳糖基转移酶将半乳糖残基转移到乳糖基神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位，以及编码该酶的DNA。提供的包括以下多肽（a）和（b ）和其编码的DNA：（a）由SEQ ID NO：2中的氨基酸编号46-353所示的氨基酸序列组成的多肽; 或（b）包含在（a）的氨基酸序列中包含一个或几个氨基酸的取代，缺失，插入或转座的氨基酸序列的多肽，并且具有从半乳糖供体转移半乳糖残基的酶活性 至作为受体的乳糖神经酰胺或半乳糖神经酰胺的半乳糖残基的C4位置。