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    Method for Screening Genomic Dna Fragments
    1.
    发明申请
    Method for Screening Genomic Dna Fragments 有权
    筛选基因组DNA片段的方法

    公开(公告)号：US20080301832A1

    公开(公告)日：2008-12-04

    申请号：US10576693

    申请日：2004-10-22

    申请人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

    发明人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

    IPC分类号： C12N15/11 ,  C12Q1/68 ,  A01H5/00 ,  C12P19/28

    CPC分类号： A01H1/00 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

    摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement.The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

    摘要翻译： 本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。 本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

    Method for screening genomic DNA fragments
    7.
    发明授权
    Method for screening genomic DNA fragments 有权
    筛选基因组DNA片段的方法

    公开(公告)号：US08039687B2

    公开(公告)日：2011-10-18

    申请号：US10576693

    申请日：2004-10-22

    申请人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

    发明人： Tomoaki Kubo ,  Toshihiko Komari ,  Satoru Usami ,  Yoshimitsu Takakura ,  Yukoh Hiei ,  Yuji Ishida

    IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/29 ,  C12N15/55 ,  C12N15/70 ,  C12N15/84

    CPC分类号： A01H1/00 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8261 ,  Y02A40/146

    摘要： The present invention provides a method for selecting genomic DNA fragments which are useful for providing a plant with an agriculturally advantageous improvement. The method of the present invention comprises the steps of: 1) preparing genomic DNA from a plant, which is then cloned into a cloning vector to form a genomic DNA library; 2) introducing a genomic fragment from each of the genomic clones constituting the genomic DNA library separately into a plant to produce transgenic plants; 3) cultivating the transgenic plants or progeny thereof to select a plant exhibiting an agriculturally advantageous phenotypic variation; and 4) selecting the genomic DNA fragment, which was introduced in step (2) into the plant selected in step (3), as a purposed genomic DNA fragment.

    摘要翻译： 本发明提供了一种选择可用于提供具有农业上有利改进的植物的基因组DNA片段的方法。 本发明的方法包括以下步骤：1）从植物制备基因组DNA，然后将其克隆到克隆载体中以形成基因组DNA文库; 2）将构成基因组DNA文库的每个基因组克隆的基因组片段分别引入植物中以产生转基因植物; 3）培育转基因植物或其后代以选择表现出农业上有利的表型变异的植物; 和4）将在步骤（2）中引入的基因组DNA片段选择在步骤（3）中选择的植物中，作为目的基因组DNA片段。

    Method of elevating photosynthesis speed of plant by improving pyruvate phosphate dikinase
    8.
    发明申请
    Method of elevating photosynthesis speed of plant by improving pyruvate phosphate dikinase 审中-公开

    公开(公告)号：US20080254536A1

    公开(公告)日：2008-10-16

    申请号：US12155372

    申请日：2008-06-03

    申请人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

    发明人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

    IPC分类号： C12N15/82 ,  C07H21/00 ,  C12N15/29

    CPC分类号： C12N9/1294 ,  C12N15/8269

    摘要： The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

    Polypeptide having cold-stable pyruvate, orthophoshate dikinase activity, DNA encoding the same and recombinant vector and transformed plants containing the DNA
    9.
    发明授权
    Polypeptide having cold-stable pyruvate, orthophoshate dikinase activity, DNA encoding the same and recombinant vector and transformed plants containing the DNA 失效
    具有冷稳定丙酮酸的多肽，正磷酸二激酶活性，编码它​​们的DNA和重组载体以及含有DNA的转化植物

    公开(公告)号：US5912156A

    公开(公告)日：1999-06-15

    申请号：US500857

    申请日：1995-08-02

    申请人： Shozo Ohta ,  Satoru Usami ,  James Nigel Burnell

    发明人： Shozo Ohta ,  Satoru Usami ,  James Nigel Burnell

    IPC分类号： C12N9/12 ,  C12N15/54 ,  C12N15/82 ,  C07H21/04 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C12N9/1294 ,  C12N15/8273

    摘要： As means for giving cold-stability to plants, a novel polypeptide having cold-stable pyruvate, orthophosphate dikinase activity, a cloned DNA encoding the same, and a recombinant vector containing the DNA are disclosed. The polypeptide according to the present invention has cold-stable pyruvate, orthophosphate dikinase activity, which has an amino acid sequence that is the same as the amino acid sequence of 1/6 region of the entire region from the C-terminal of the following polypeptide (1) or (2), except that at least one amino acid residues of said 1/6 region are substituted with other amino acid residues:(1) a pyruvate, orthophosphate dikinase having an amino acid sequence shown in SEQ ID NOS. 1-10 in Sequence Listing; and(2) a polypeptide having an amino acid sequence which has a homology of not less than 50% with the amino acid sequence mentioned in (1), said polypeptide having cold-stable pyruvate, orthophosphate dikinase activity.

    摘要翻译： PCT No.PCT / JP94 / 02022 Sec。 371日期：1995年8月2日 102（e）日期1995年8月2日PCT 1994年12月1日PCT PCT。 WO95 / 15385 PCT公开 日期：1995年6月8日作为赋予植物冷稳定性的手段，公开了具有冷稳定丙酮酸，正磷酸二激酶活性的新型多肽，编码该多肽的克隆DNA，以及含有该DNA的重组载体。 根据本发明的多肽具有冷稳定的丙酮酸，正磷酸二激酶活性，其具有与C的整个区域的+ E，fra 1/6 + EE区的氨基酸序列相同的氨基酸序列 （1）或（2）的末端，除了所述+ E，fra 1/6 + EE区的至少一个氨基酸残基被其它氨基酸残基取代：（1）丙酮酸，正磷酸二激酶 具有SEQ ID NOS所示的氨基酸序列。 1-10序列表; 和（2）具有与（1）中提及的氨基酸序列具有不小于50％同源性的氨基酸序列的多肽，所述多肽具有冷稳定的丙酮酸，正磷酸二激酶活性。