公开(公告)号：US20080254536A1

公开(公告)日：2008-10-16

申请号：US12155372

申请日：2008-06-03

申请人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

发明人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

IPC分类号： C12N15/82 ,  C07H21/00 ,  C12N15/29

CPC分类号： C12N9/1294 ,  C12N15/8269

摘要： The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

公开(公告)号：US07396977B2

公开(公告)日：2008-07-08

申请号：US10493537

申请日：2002-10-23

申请人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

发明人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

IPC分类号： C12N15/82

CPC分类号： C12N9/1294 ,  C12N15/8269

摘要： The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

摘要翻译： 本发明涉及C4植物的转化。 它也涉及C4植物中外源基因的高水平表达。 更具体地，本发明涉及通过实现构成C4光合途径的酶的高水平表达来产生在低温下保持优异光合能力的C4植物。 在本发明中，使用包含启动子，C4植物基因组基因的表达盒，在所述启动子的控制下，编码构成光合途径的酶和终止子的转化C4植物。 编码构成光合途径的酶的C4植物基因组基因优选为C4植物基因组衍生的PPDK基因或其修饰形式。 本发明特别可用于改进具有PPDK的C4植物的生产（特别是在改善低温条件下玉米的生产中）。

公开(公告)号：US20060064783A1

公开(公告)日：2006-03-23

申请号：US10493537

申请日：2002-10-23

申请人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

发明人： Satoru Usami ,  Shozo Ohta ,  Yuji Ishida

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N15/82

CPC分类号： C12N9/1294 ,  C12N15/8269

摘要： The present invention relates to the transformation of C4 plants. It also relates to the high-level expression of foreign genes in C4 plants. More specifically, the present invention relates to the creation of C4 plants retaining an excellent photosynthetic capacity at low temperature by achieving high-level expression of an enzyme constituting the C4 photosynthetic pathway. In the present invention, C4 plants are transformed using an expression cassette that comprises a promoter, a C4 plant genomic gene, under control of said promoter, encoding an enzyme constituting a photosynthetic pathway, and a terminator. The C4 plant genomic gene encoding an enzyme constituting a photosynthetic pathway is preferably a C4 plant genome-derived PPDK gene or a modified form thereof. The present invention is particularly useful in improving the production of C4 plants having PPDK (especially, in improving the production of maize under low temperature conditions).

摘要翻译： 本发明涉及C4植物的转化。 它也涉及C4植物中外源基因的高水平表达。 更具体地，本发明涉及通过实现构成C4光合途径的酶的高水平表达来产生在低温下保持优异光合能力的C4植物。 在本发明中，使用包含启动子，C4植物基因组基因的表达盒，在所述启动子的控制下，编码构成光合途径的酶和终止子的转化C4植物。 编码构成光合途径的酶的C4植物基因组基因优选为C4植物基因组衍生的PPDK基因或其修饰形式。 本发明特别可用于改进具有PPDK的C4植物的生产（特别是在改善低温条件下玉米的生产中）。

公开(公告)号：US5912156A

公开(公告)日：1999-06-15

申请号：US500857

申请日：1995-08-02

申请人： Shozo Ohta ,  Satoru Usami ,  James Nigel Burnell

发明人： Shozo Ohta ,  Satoru Usami ,  James Nigel Burnell

IPC分类号： C12N9/12 ,  C12N15/54 ,  C12N15/82 ,  C07H21/04 ,  C12N5/00 ,  C12N15/00

CPC分类号： C12N9/1294 ,  C12N15/8273

摘要： As means for giving cold-stability to plants, a novel polypeptide having cold-stable pyruvate, orthophosphate dikinase activity, a cloned DNA encoding the same, and a recombinant vector containing the DNA are disclosed. The polypeptide according to the present invention has cold-stable pyruvate, orthophosphate dikinase activity, which has an amino acid sequence that is the same as the amino acid sequence of 1/6 region of the entire region from the C-terminal of the following polypeptide (1) or (2), except that at least one amino acid residues of said 1/6 region are substituted with other amino acid residues:(1) a pyruvate, orthophosphate dikinase having an amino acid sequence shown in SEQ ID NOS. 1-10 in Sequence Listing; and(2) a polypeptide having an amino acid sequence which has a homology of not less than 50% with the amino acid sequence mentioned in (1), said polypeptide having cold-stable pyruvate, orthophosphate dikinase activity.

摘要翻译： PCT No.PCT / JP94 / 02022 Sec。 371日期：1995年8月2日 102（e）日期1995年8月2日PCT 1994年12月1日PCT PCT。 WO95 / 15385 PCT公开 日期：1995年6月8日作为赋予植物冷稳定性的手段，公开了具有冷稳定丙酮酸，正磷酸二激酶活性的新型多肽，编码该多肽的克隆DNA，以及含有该DNA的重组载体。 根据本发明的多肽具有冷稳定的丙酮酸，正磷酸二激酶活性，其具有与C的整个区域的+ E，fra 1/6 + EE区的氨基酸序列相同的氨基酸序列 （1）或（2）的末端，除了所述+ E，fra 1/6 + EE区的至少一个氨基酸残基被其它氨基酸残基取代：（1）丙酮酸，正磷酸二激酶 具有SEQ ID NOS所示的氨基酸序列。 1-10序列表; 和（2）具有与（1）中提及的氨基酸序列具有不小于50％同源性的氨基酸序列的多肽，所述多肽具有冷稳定的丙酮酸，正磷酸二激酶活性。

公开(公告)号：US06610913B1

公开(公告)日：2003-08-26

申请号：US09155989

申请日：1998-12-10

申请人： Masao Arai ,  Shoichi Suzuki ,  Nobuhiko Murai ,  Shigehiro Yamada ,  Shozo Ohta ,  James N. Burnell

发明人： Masao Arai ,  Shoichi Suzuki ,  Nobuhiko Murai ,  Shigehiro Yamada ,  Shozo Ohta ,  James N. Burnell

IPC分类号： C12N1529

CPC分类号： C12N9/88 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/8269

摘要： The present invention relates a method for transforming a rice plant to provide it with a C4 photosynthetic pathway by way of the introduction of some genes participating in a C4 photosynthetic pathway. To this end, the method of the invention comprises introducing a phosphoenolpyruvate carboxylase (PEPC) and a gene coding for a phosphoenolpyruvate carboxykinase (PCK) which has been connected with a DNA fragment coding for a transit peptide into a rice plant.

摘要翻译： 本发明涉及通过引入参与C4光合途径的一些基因来转化水稻植物以提供C4光合途径的方法。 为此，本发明的方法包括将磷酸烯醇丙酮酸羧化酶（PEPC）和编码与转运肽的DNA片段连接的水稻植物中的编码磷酸烯醇丙酮酸羧激酶（PCK）的基因导入。

公开(公告)号：US20060253931A1

公开(公告)日：2006-11-09

申请号：US10489915

申请日：2002-09-13

申请人： Toshiyuki Komori ,  Shozo Ohta ,  Nobuhiko Murai ,  Yukoh Hiei

发明人： Toshiyuki Komori ,  Shozo Ohta ,  Nobuhiko Murai ,  Yukoh Hiei

IPC分类号： A01H5/00 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8289 ,  C07K14/415

摘要： The purpose of the present invention is to provide a method for providing and inhibiting the rice fertility to the rice BT type cytoplasmic male sterility, and discerning the presence of the rice restorer gene. The present invention employs a nucleic acid having the base sequence of SEQ ID NO.27, or a nucleic acid having a base sequence which is identical to at least 70% of the base sequence of SEQ ID NO.27, and which functions to restore fertility. Alternatively, a nucleic acid having the base sequence of bases 38538-54123 of SEQ ID NO.27, or having a base sequence which is identical to at least 70% of the base sequence of bases 38538-54123 of SEQ ID NO.27, and which functions to restore fertility, is used.

摘要翻译： 本发明的目的是提供一种提供和抑制水稻BT型细胞质雄性不育的水稻育性的方法，并且鉴别水稻恢复基因的存在。 本发明使用具有SEQ ID NO.27的碱基序列的核酸或具有与SEQ ID NO：27的碱基序列的至少70％相同的碱基序列的核酸，并且其功能恢复 生育力 或者，具有SEQ ID NO：27的碱基序列38538-54123或具有与SEQ ID NO.27的碱基序列38538-54123的至少70％相同的碱基序列的核酸， 并使用恢复生育能力的功能。

公开(公告)号：US06214578B1

公开(公告)日：2001-04-10

申请号：US09011526

申请日：1998-02-12

申请人： Jun Ueki ,  Shozo Ohta ,  Shinji Morioka ,  Yoshiki Kuraya

发明人： Jun Ueki ,  Shozo Ohta ,  Shinji Morioka ,  Yoshiki Kuraya

IPC分类号： C12N1582

CPC分类号： C12N9/0065 ,  C07K14/415 ,  C12N9/16 ,  C12N15/67 ,  C12N15/8216

摘要： A method for expressing a foreign gene, by which the foreign genes can be expressed more strongly than by the conventional methods, as well as a recombinant vector therefor, is disclosed. In the method for expressing a foreign gene according to the present invention, the foreign gene is inserted into a site downstream of a promoter and the foreign gene is expressed in a cell, wherein a plurality of intron-originated DNA fragments which are the same or different and are capable of promoting expression of foreign genes are inserted into one or more sites upstream of the foreign gene, and said foreign gene is expressed.

摘要翻译： 公开了一种表达外源基因的方法，通过该外源基因可以比常规方法更强烈地表达外源基因，以及其重组载体。 在本发明的外源基因表达方法中，将外源基因插入到启动子下游的位点，外源基因在细胞中表达，其中多个内含子起始的DNA片段相同或 不同的并能促进外源基因表达插入外源基因上游一个或多个位点，并且表达外源基因。

公开(公告)号：US5088432A

公开(公告)日：1992-02-18

申请号：US436047

申请日：1989-11-14

申请人： Masahiro Usami ,  Kenji Ueda ,  Kiyomi Tomoshige ,  Shozo Ohta ,  Tsutomu Horiguchi ,  Hiroshi Yamazaki

发明人： Masahiro Usami ,  Kenji Ueda ,  Kiyomi Tomoshige ,  Shozo Ohta ,  Tsutomu Horiguchi ,  Hiroshi Yamazaki

IPC分类号： C09D5/14 ,  B63B59/04 ,  C09D5/16

CPC分类号： B63B59/04 ,  Y02T70/121 ,  Y02T70/123

摘要： The current invention relates to an anti-fouling system for substances in contact with seawater which is constituted of the first conductive membrane that is coated on the outer side of the electric insulator mounted at the surface of the substance such as ships, marine structures in contact with seawater and is composed of thin sheets of metals having low specific resistance or metal oxide, spray-coated membrane, evaporated membrane or fused membrane, and the second conductive anti-fouling membrane having higher electric resistance than the first conductive membrane which is coated on the outer side of the above first conductive membrane and which is composed of the oxidation-resistant insoluble substance and the organic binder, and the power supply equipment that supplies direct currents flowing from the first conductive membrane through the second conductive membrane toward the electric conductor made of iron, copper or carbon located in seawater and opposite the second conductive membrane, owing to the constitution of which the prevention of rise in resistance because of consumption of the conductive membrane, the uniforming of current distribution and a high anti-fouling performance by using the low-resistant conductive membrane are achieved.