公开(公告)号：US20090240034A1

公开(公告)日：2009-09-24

申请号：US12091372

申请日：2006-10-25

申请人： Shino Manabe ,  Yukishige Ito ,  Tomoyuki Sugioka

发明人： Shino Manabe ,  Yukishige Ito ,  Tomoyuki Sugioka

IPC分类号： C07K1/107

CPC分类号： C07K9/005 ,  C07K1/04 ,  C07K5/0606 ,  C07K5/1013 ,  C07K7/06

摘要： It is an object of the present invention to provide a novel method for producing a peptide thioester. In the present invention, general peptide synthesis is performed on a solid-phase resin, carboxylic acid obtained after cutout is allowed to react with p-toluenesulfonyl isocyanate, and then the reaction product is alkylated, and is reacted with thiol. Thus, peptide thioester is simply synthesized under mild conditions.

摘要翻译： 本发明的目的是提供一种制备肽硫酯的新方法。 在本发明中，对固相树脂进行一般的肽合成，在将裂纹与对甲苯磺酰基异氰酸酯反应后得到的羧酸，然后将反应产物烷基化，并与硫醇反应。 因此，肽硫酯在温和条件下简单合成。

公开(公告)号：US08124789B2

公开(公告)日：2012-02-28

申请号：US12329844

申请日：2008-12-08

申请人： Shino Manabe ,  Yukishige Ito ,  Yoshito Ihara

发明人： Shino Manabe ,  Yukishige Ito ,  Yoshito Ihara

IPC分类号： A61K39/395 ,  C07D209/20 ,  C07D403/14 ,  C07K16/00

CPC分类号： C07K16/44

摘要： This invention provides a method of producing a large amount of an α-form or β-form of N-pyranosyl-tryptophan having a specified structure and preparing an antibody therefrom. The method comprises a step of allowing a pyranose compound to react with a 3-pyrazyl-indole compound in the presence of a first base to obtain a 1-pyranosyl-3-pyrazyl-indole compound, and a step of treating the 1-pyranosyl-3-pyrazyl-indole compound with an acid, treating the resultant with a hydrogenation catalyst, and further treating the resultant with a second base to synthesize N-pyranosyl-tryptophan, thereby producing an antibody therefrom.

摘要翻译： 本发明提供一种生产具有特定结构并由其制备抗体的大量α-形式或“形式”的N-吡喃糖基 - 色氨酸的方法。 该方法包括使吡喃糖化合物与3-吡嗪基 - 吲哚化合物在第一碱的存在下反应得到1-吡喃糖基-3-吡嗪基 - 吲哚化合物的步骤，以及将1-吡喃糖基 -3-吡唑基 - 吲哚化合物与酸反应，用氢化催化剂处理所得物，并用第二碱处理所得产物以合成N-吡喃糖基 - 色氨酸，从而由此产生抗体。

公开(公告)号：US07879766B2

公开(公告)日：2011-02-01

申请号：US11723933

申请日：2007-03-22

申请人： Ichiro Matsuo ,  Yukishige Ito

发明人： Ichiro Matsuo ,  Yukishige Ito

IPC分类号： C12N9/24 ,  C07H1/00 ,  C07H3/00 ,  C12P19/18

CPC分类号： C12P19/20 ,  C07H1/00 ,  C07H3/00

摘要： The present invention provides methods which enables synthesis of various sugar chains and products obtained by the same. More specifically, the present invention provides protected sugar chain compounds represented by the formula (I) below: [wherein R1 and R2 are the same or different and each is a linear or branched sugar chain, S1 is any sugar residue, SA and SB are the same or different sugar residues, L is a bond or a linear sugar chain, X is absent, or, if present, represents certain group, the sugar residues SA and SB are cleaved by different exoglycosidases, respectively] and libraries thereof, and methods of producing the same; methods of producing a sugar chain compound, which comprises treating the sugar chain compound or library with glycosidase, and glycosidase decomposition products obtained by the same; intermediates for the synthesis of protected sugar chain compounds; reagents and kits; and the like.

摘要翻译： 本发明提供能够合成各种糖链的方法和由其获得的产品。 更具体地，本发明提供由下式（I）表示的受保护的糖链化合物：[其中R1和R2相同或不同，各自为直链或支链糖链，S1为任何糖残基，SA和SB为 相同或不同的糖残基，L是键或直链糖链，X不存在，或者如果存在，则代表某一组，糖残基SA和SB分别被不同的外切糖苷酶切割）及其文库，以及方法 生产同样的; 制造糖链化合物的方法，其包括用糖苷酶处理糖链化合物或文库，以及由其获得的糖苷酶分解产物; 用于合成受保护的糖链化合物的中间体; 试剂和试剂盒; 等等。

公开(公告)号：US20070208171A1

公开(公告)日：2007-09-06

申请号：US11680248

申请日：2007-02-28

申请人： Shino MANABE ,  Yukishige ITO ,  Kazuyuki ISHII

发明人： Shino MANABE ,  Yukishige ITO ,  Kazuyuki ISHII

IPC分类号： C08B37/00 ,  C07H5/06

CPC分类号： C07H9/06 ,  Y02P20/55

摘要： The present invention provides a compound represented by the following formula (1): wherein X1 and X2 each independently represent a hydrogen atom or a hydroxyl-protecting group; Y represents a C7-20 aralkyl group which may optionally have one or more substituents selected from a halogen atom, a lower alkyl group or a lower alkoxy group; and Z represents a halogen atom, a C1-4 alkylthio, or an arylthio group, or its corresponding sulfoxide group.

摘要翻译： 本发明提供由下式（1）表示的化合物：其中X1和X2各自独立地表示氢原子或羟基保护基; Y表示可任选具有一个或多个选自卤素原子，低级烷基或低级烷氧基的取代基的C7-20芳烷基; Z表示卤素原子，C1-4烷硫基或芳硫基，或其相应的亚砜基。

公开(公告)号：US20070254344A1

公开(公告)日：2007-11-01

申请号：US11723933

申请日：2007-03-22

申请人： Ichiro Matsuo ,  Yukishige Ito

发明人： Ichiro Matsuo ,  Yukishige Ito

IPC分类号： C12N9/24 ,  C07H1/00 ,  C12P19/18 ,  C07H3/00

CPC分类号： C12P19/20 ,  C07H1/00 ,  C07H3/00

摘要： The present invention provides methods which enables synthesis of various sugar chains and products obtained by the same. More specifically, the present invention provides protected sugar chain compounds represented by the formula (I) below: [wherein R1 and R2 are the same or different and each is a linear or branched sugar chain, S1 is any sugar residue, SA and SB are the same or different sugar residues, L is a bond or a linear sugar chain, X is absent, or, if present, represents certain group, the sugar residues SA and SB are cleaved by different exoglycosidases, respectively] and libraries thereof, and methods of producing the same; methods of producing a sugar chain compound, which comprises treating the sugar chain compound or library with glycosidase, and glycosidase decomposition products obtained by the same; intermediates for the synthesis of protected sugar chain compounds; reagents and kits; and the like.

摘要翻译： 本发明提供能够合成各种糖链的方法和由其获得的产品。 更具体地，本发明提供由下式（I）表示的受保护的糖链化合物：[其中R 1和R 2相同或不同，并且各自为 直链或支链糖链，S 1是任何糖残基，S和A和S B是相同或不同的糖残基，L是 键或线性糖链，X不存在，或者如果存在，则代表某一组，分别由不同的外切糖苷酶切割糖残基S A和S B] 及其文库及其制备方法; 制造糖链化合物的方法，其包括用糖苷酶处理糖链化合物或文库，以及由其获得的糖苷酶分解产物; 用于合成受保护的糖链化合物的中间体; 试剂和试剂盒; 等等。

公开(公告)号：US20140024825A1

公开(公告)日：2014-01-23

申请号：US14008694

申请日：2012-03-19

申请人： Hidekazu Kano ,  Yukishige Ito ,  Yoshitaka Aranishi

发明人： Hidekazu Kano ,  Yukishige Ito ,  Yoshitaka Aranishi

IPC分类号： C08B3/08

CPC分类号： C08B3/08 ,  C08B3/16 ,  C08B13/00

摘要： A manufacturing method for an esterification product of a cellulose derivative by comprising esterification of a cellulose derivative in a mixture of an ionic liquid, cellulose derivative and agent that is at least one selected from the group consisting of carboxylic anhydride, carboxylic acid halide, and carboxylic acid.

摘要翻译： 纤维素衍生物的酯化产物的制造方法，其包括在离子液体，纤维素衍生物和选自羧酸酐，羧酸卤化物和羧酸中的至少一种的化合物的混合物中纤维素衍生物的酯化 酸。

公开(公告)号：US08524873B2

公开(公告)日：2013-09-03

申请号：US11680248

申请日：2007-02-28

申请人： Shino Manabe ,  Yukishige Ito ,  Kazuyuki Ishii

发明人： Shino Manabe ,  Yukishige Ito ,  Kazuyuki Ishii

IPC分类号： C07G3/00 ,  C07H1/00

CPC分类号： C07H9/06 ,  Y02P20/55

摘要： The present invention provides a compound represented by the following formula (1): wherein X1 and X2 each independently represent a hydrogen atom or a hydroxyl-protecting group; Y represents a C7-20 aralkyl group which may optionally have one or more substituents selected from a halogen atom, a lower alkyl group or a lower alkoxy group; and Z represents a halogen atom, a C1-4 alkylthio, or an arylthio group, or its corresponding sulfoxide group.

摘要翻译： 本发明提供由下式（1）表示的化合物：其中X1和X2各自独立地表示氢原子或羟基保护基; Y表示可任选具有一个或多个选自卤素原子，低级烷基或低级烷氧基的取代基的C7-20芳烷基; Z表示卤素原子，C1-4烷硫基或芳硫基，或其相应的亚砜基。

公开(公告)号：US08076299B2

公开(公告)日：2011-12-13

申请号：US12091372

申请日：2006-10-25

申请人： Shino Manabe ,  Yukishige Ito ,  Tomoyuki Sugioka

发明人： Shino Manabe ,  Yukishige Ito ,  Tomoyuki Sugioka

IPC分类号： A61K38/16 ,  A61K38/14 ,  C10M135/06

CPC分类号： C07K9/005 ,  C07K1/04 ,  C07K5/0606 ,  C07K5/1013 ,  C07K7/06

摘要： It is an object of the present invention to provide a novel method for producing a peptide thioester. In the present invention, general peptide synthesis is performed on a solid-phase resin, carboxylic acid obtained after cutout is allowed to react with p-toluenesulfonyl isocyanate, and then the reaction product is alkylated, and is reacted with thiol. Thus, peptide thioester is simply synthesized under mild conditions.

摘要翻译： 本发明的目的是提供一种制备肽硫酯的新方法。 在本发明中，对固相树脂进行一般的肽合成，在将裂纹与对甲苯磺酰基异氰酸酯反应后得到的羧酸，然后将反应产物烷基化，并与硫醇反应。 因此，肽硫酯在温和条件下简单合成。

公开(公告)号：US20100028329A1

公开(公告)日：2010-02-04

申请号：US12329844

申请日：2008-12-08

申请人： Shino MANABE ,  Yukishige ITO ,  Yoshito IHARA

发明人： Shino MANABE ,  Yukishige ITO ,  Yoshito IHARA

IPC分类号： A61K39/395 ,  C07D209/20 ,  C07D403/14 ,  C07K16/00 ,  A61P37/04

CPC分类号： C07K16/44

摘要： This invention provides a method of producing a large amount of an α-form or β-form of N-pyranosyl-tryptophan having a specified structure and preparing an antibody therefrom. The method comprises a step of allowing a pyranose compound to react with a 3-pyrazyl-indole compound in the presence of a first base to obtain a 1-pyranosyl-3-pyrazyl-indole compound, and a step of treating the 1-pyranosyl-3-pyrazyl-indole compound with an acid, treating the resultant with a hydrogenation catalyst, and further treating the resultant with a second base to synthesize N-pyranosyl-tryptophan, thereby producing an antibody therefrom.

摘要翻译： 本发明提供了生产具有特定结构并由其制备抗体的大量α-形式或β-形式的N-吡喃糖基 - 色氨酸的方法。 该方法包括使吡喃糖化合物与3-吡嗪基 - 吲哚化合物在第一碱的存在下反应得到1-吡喃糖基-3-吡嗪基 - 吲哚化合物的步骤，以及将1-吡喃糖基 -3-吡唑基 - 吲哚化合物与酸反应，用氢化催化剂处理所得物，并用第二碱处理所得产物以合成N-吡喃糖基 - 色氨酸，从而由此产生抗体。

公开(公告)号：US5409817A

公开(公告)日：1995-04-25

申请号：US57528

申请日：1993-05-04

申请人： Yukishige Ito ,  James C. Paulson

发明人： Yukishige Ito ,  James C. Paulson

IPC分类号： C12N9/10 ,  C12N9/12 ,  C12N9/24 ,  C12P19/26 ,  C12P19/44 ,  C12N11/00 ,  C12P19/00 ,  C12P19/18

CPC分类号： C12N9/1241 ,  C12N9/1081 ,  C12N9/2402 ,  C12P19/26 ,  C12Y302/01018

摘要： A single vessel cyclic synthesis process for preparation of a sialyl.alpha.2.fwdarw.3.beta.galactoside is disclosed. In accordance with this process, a sialyltransferase acceptor is sialylated in an aqueous reaction medium by an .alpha.(2,3)sialyl transferase and CMP-sialic acid to form a sialyl donor substrate and CMP. In the presence of the trans-sialidase of Trypanosoma crusi, that sialyl donor substrate provides a sialyl group for a trans-sialidase acceptor, thereby preparing the sialyl.alpha.2.fwdarw.3.beta.galactoside. The .alpha.(2,3)sialyltransferase acceptor is reformed upon trans-sialidation of the latter acceptor, and the sialyl donor substrate is reformed using the .alpha.(2,3)sialyltransferase and a CMP-sialic acid recycling system that combines CMP with sialic acid that is also present in the vessel. The K.sub.m /V.sub.max value for the .alpha.(2,3)sialyltransferase acceptor is less than one-tenth the value of K.sub.m /V.sub.max of the trans-sialidase acceptor for the .alpha.(2,3)sialyltransferase.

摘要翻译： 公开了用于制备唾液酸α2->3β半乳糖苷的单容器循环合成方法。 根据该方法，唾液酸转移酶受体通过α（2,3）唾液酸转移酶和CMP-唾液酸在水性反应介质中唾液酸化以形成唾液酸供体底物和CMP。 在克氏锥虫的唾液酸酶的存在下，唾液酸供体底物为唾液酸酶受体提供唾液酸基，从而制备唾液酸α2->3β半乳糖苷。 将α（2,3）唾液酸转移酶受体通过后一个受体的反式唾液酸化进行重组，并且使用α（2,3）唾液酸转移酶和将CMP与唾液酸组合的CMP-唾液酸回收系统重组唾液酸供体底物 这也存在于船上。 α（2,3）唾液酸转移酶受体的Km / Vmax值小于α（2,3）唾液酸转移酶的唾液酸酶受体的Km / Vmax值的十分之一。