公开(公告)号：US08518686B2

公开(公告)日：2013-08-27

申请号：US12765825

申请日：2010-04-22

申请人： Zachary Quinn Beck ,  Richard R. Bott ,  Christopher Lee Rife ,  Derek H. Wells ,  Jeffrey V. Miller

发明人： Zachary Quinn Beck ,  Richard R. Bott ,  Christopher Lee Rife ,  Derek H. Wells ,  Jeffrey V. Miller

IPC分类号： C12N1/20 ,  C12N9/88 ,  C12N1/00 ,  C12N15/00 ,  C12P5/02 ,  C07H21/04

CPC分类号： C12P5/007 ,  C07K2299/00 ,  C12N9/88 ,  C12Y402/03027

摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

公开(公告)号：US20110076743A1

公开(公告)日：2011-03-31

申请号：US12765825

申请日：2010-04-22

申请人： Zachary Quinn Beck ,  Richard R. Bott ,  Christopher Lee Rife ,  Derek H. Wells ,  Jeffrey V. Miller

发明人： Zachary Quinn Beck ,  Richard R. Bott ,  Christopher Lee Rife ,  Derek H. Wells ,  Jeffrey V. Miller

IPC分类号： C12N9/88 ,  C12N5/10

CPC分类号： C12P5/007 ,  C07K2299/00 ,  C12N9/88 ,  C12Y402/03027

摘要： The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

摘要翻译： 本发明提供了三叶虫异戊二烯合酶和异戊二烯异戊二烯合酶的三维结构。 本发明还提供使用三维结构设计异戊二烯合酶的方法，所述异戊二烯合酶具有改进的微生物宿主细胞中异戊二烯生产增加的活性。 本发明的生物合成生产的异戊二烯可用于制造橡胶和弹性体。

公开(公告)号：US20100184178A1

公开(公告)日：2010-07-22

申请号：US12560305

申请日：2009-09-15

申请人： Zachary Quinn Beck ,  Anthony Rudolf Calabria ,  Michael Charles Miller ,  Dmitrii V. Vaviline ,  Derek H. Wells

发明人： Zachary Quinn Beck ,  Anthony Rudolf Calabria ,  Michael Charles Miller ,  Dmitrii V. Vaviline ,  Derek H. Wells

IPC分类号： C12P5/02 ,  C12N9/12

CPC分类号： C12P5/007 ,  C12N9/1085 ,  C12N9/1205 ,  C12P7/04 ,  C12Y207/01036

摘要： The invention features methods for producing isoprene from cultured cells having increased expression levels and/or activity levels of a mevalonate kinase polypeptide and an isoprene synthase polypeptide. The invention also provides methods for producing isoprene from cultured cells having reduced accumulation of intermediates (such as mevalonate, isopentenyl diphosphate, 3,3-dimethylallyl diphosphate, geranyl diphosphate, or farnesyl diphosphate) in the biosynthesis of isoprene or isoprenoids that may otherwise cause undesirable amounts of growth inhibition, toxicity, or cell death. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

摘要翻译： 本发明的特征在于具有增加的甲羟戊酸激酶多肽和异戊二烯合酶多肽的表达水平和/或活性水平的培养细胞产生异戊二烯的方法。 本发明还提供了在异戊二烯或类异戊二烯的生物合成中具有减少的中间体（例如甲羟戊酸，异戊烯基二磷酸，3,3-二甲基烯丙基二磷酸酯，香叶基二磷酸酯或法呢基二磷酸酯）积累减少的培养细胞的方法。 生长抑制，毒性或细胞死亡的量。 得到的异戊二烯组合物可以具有增加的异戊二烯的产率和/或纯度。