公开(公告)号：US20250147036A1

公开(公告)日：2025-05-08

申请号：US18708833

申请日：2022-11-09

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Camilla ENGBLOM ,  Kim THRANE ,  Jeffrey MOLD ,  Jonas FRISEN ,  Joakim LUNDBERG ,  Qirong LIN ,  Caroline Julie GALLANT ,  Marlon STOECKIUS ,  Katherine PFEIFFER ,  Zachary BENT ,  Christina GALONSKA ,  Ariel ROYALL

IPC: G01N33/68 ,  C12N15/10

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

公开(公告)号：US20220228210A1

公开(公告)日：2022-07-21

申请号：US17565047

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Denis PRISTINSKI ,  Preyas SHAH ,  Steven William SHORT ,  Dieter WILK ,  Siyuan XING

IPC: C12Q1/6874 ,  C12N15/10

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

公开(公告)号：US20240167077A1

公开(公告)日：2024-05-23

申请号：US18215299

申请日：2023-06-28

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Kyle VANDERSCHOOT

IPC: C12Q1/6806 ,  C12Q1/6874

CPC classification number: C12Q1/6806 ,  C12Q1/6874 ,  C12Q2600/166

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photocontrollable ligation of nucleic acid molecules with splints, wherein at least one of the splints comprises a photo-crosslinkable moiety. Photo-induced crosslinking of the splint to oligonucleotide molecules immobilized on a substrate allows the splint to remain attached to the oligonucleotide molecules during subsequent rounds of photoresist application and removal. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

公开(公告)号：US20240076656A1

公开(公告)日：2024-03-07

申请号：US18215296

申请日：2023-06-28

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Steven William SHORT ,  Dieter WILK ,  Pingwei ZHUANG ,  Solongo Batjargal ZIRALDO

IPC: C12N15/10 ,  B01J19/00 ,  B41M5/24 ,  C12N9/12 ,  C12N9/22 ,  G03F7/11

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B41M5/24 ,  C12N9/1264 ,  C12N9/22 ,  C12Y207/07031 ,  C12Y301/11001 ,  G03F7/11 ,  B01J2219/00855 ,  B01J2219/00927 ,  B01J2219/00993 ,  B01J2219/0894 ,  B01J2540/68

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate comprising a step of blocking or ablating oligonucleotide molecules in a boundary region separating a plurality of spot regions, and attaching oligonucleotides to oligonucleotide molecules in a first one or more of the spot regions.

公开(公告)号：US20240076722A1

公开(公告)日：2024-03-07

申请号：US18342952

申请日：2023-06-28

Applicant: 10x Genomics, Inc.

Inventor： David Michael PATTERSON ,  Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN

IPC: C12Q1/6837 ,  B01L3/00

CPC classification number: C12Q1/6837 ,  B01L3/502761 ,  C12Q2600/16 ,  B01L2200/0652

Abstract: The present disclosure relates in some aspects to methods and compositions for processes for inverting oligonucleotide molecules in an in situ synthesized array, including reversing the orientation of the oligonucleotide molecules with respect to the array substrate from 3′-immobilized to 5′-immobilized.

公开(公告)号：US20230314445A1

公开(公告)日：2023-10-05

申请号：US18052537

申请日：2022-11-03

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON

IPC: G01N33/68 ,  G01N33/58

CPC classification number: G01N33/6854 ,  G01N33/58 ,  G01N2800/52

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

公开(公告)号：US20240027468A1

公开(公告)日：2024-01-25

申请号：US18236783

申请日：2023-08-22

Applicant: 10X GENOMICS, INC.

Inventor： David Michael PATTERSON

IPC: G01N33/68 ,  G01N33/53

CPC classification number: G01N33/6842 ,  G01N33/5308 ,  G01N2440/38 ,  G01N2333/91091 ,  G01N2570/00

Abstract: The present disclosure relates to methods, compositions, systems, and kits for detecting and analyzing the glycosylation of healthy and diseased cells and protein-specific glycosylation patterns using single-cell profiling methodologies.

公开(公告)号：US20230304072A1

公开(公告)日：2023-09-28

申请号：US18187981

申请日：2023-03-22

Applicant: 10x Genomics, Inc.

Inventor： Shalini GOHIL ,  Shea Thompson LANCE ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE

IPC: C12Q1/6806 ,  C12N15/10 ,  C08J3/075 ,  B01J13/02

CPC classification number: C12Q1/6806 ,  B01J13/02 ,  C08J3/075 ,  C12N15/1082 ,  C08J2333/26

Abstract: The present disclosure relates generally to compositions and methods for generating hydrogel coatings on features. In some embodiments, hydrogel-coated features are prepared by horseradish peroxidase-mediated gelation of microspheres. Some embodiments relate to methods for generating hydrogel coatings using particle templated emulsification. The disclosure also relates to the use of hydrogel-coated features in arrays for spatial analysis of biological analytes in biological samples.

公开(公告)号：US20220228201A1

公开(公告)日：2022-07-21

申请号：US17565028

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE ,  Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN

IPC: C12Q1/6837 ,  C12Q1/6869

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, provided herein is photocontrollable hybridization, where oligonucleotides with photo-caged bases prevent hybridization of complementary nucleic acid strands. Uncaging of the oligonucleotides allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.