公开(公告)号：US20230340565A1

公开(公告)日：2023-10-26

申请号：US18097989

申请日：2023-01-17

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila BELHOCINE ,  Josephine Lee ,  Francesca MESCHI ,  Luz MONTESCLAROS ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Jerald SAPIDA ,  David SUKOVICH ,  Tobias Daniel WHEELER ,  Yifeng YIN

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20190233878A1

公开(公告)日：2019-08-01

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20240271183A1

公开(公告)日：2024-08-15

申请号：US18542404

申请日：2023-12-15

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  B01J13/0065 ,  B01J13/18 ,  C08J3/075 ,  C08J2333/00 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20220364150A1

公开(公告)日：2022-11-17

申请号：US17861503

申请日：2022-07-11

Applicant: 10x Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20230304072A1

公开(公告)日：2023-09-28

申请号：US18187981

申请日：2023-03-22

Applicant: 10x Genomics, Inc.

Inventor： Shalini GOHIL ,  Shea Thompson LANCE ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE

IPC: C12Q1/6806 ,  C12N15/10 ,  C08J3/075 ,  B01J13/02

CPC classification number: C12Q1/6806 ,  B01J13/02 ,  C08J3/075 ,  C12N15/1082 ,  C08J2333/26

Abstract: The present disclosure relates generally to compositions and methods for generating hydrogel coatings on features. In some embodiments, hydrogel-coated features are prepared by horseradish peroxidase-mediated gelation of microspheres. Some embodiments relate to methods for generating hydrogel coatings using particle templated emulsification. The disclosure also relates to the use of hydrogel-coated features in arrays for spatial analysis of biological analytes in biological samples.

公开(公告)号：US20220228201A1

公开(公告)日：2022-07-21

申请号：US17565028

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： Geoffrey MCDERMOTT ,  David Michael PATTERSON ,  Andrew D. PRICE ,  Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN

IPC: C12Q1/6837 ,  C12Q1/6869

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, provided herein is photocontrollable hybridization, where oligonucleotides with photo-caged bases prevent hybridization of complementary nucleic acid strands. Uncaging of the oligonucleotides allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.

公开(公告)号：US20250067730A1

公开(公告)日：2025-02-27

申请号：US18932448

申请日：2024-10-30

Applicant: 10x Genomics, Inc.

Inventor： Andrew D. PRICE ,  Christopher Hindson

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  C40B30/04 ,  C40B50/06 ,  C40B70/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

Abstract: The present disclosure provides systems and methods for processing or analyzing a sample. Methods may include generating supports (e.g., beads) comprising barcode molecules coupled thereto. A support comprising barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

公开(公告)号：US20220314187A1

公开(公告)日：2022-10-06

申请号：US17565038

申请日：2021-12-29

Applicant: 10x Genomics, Inc.

Inventor： Andrew D. PRICE

IPC: B01J19/00 ,  C12Q1/6834

Abstract: Provided in some aspects are methods for light-controlled in situ surface patterning of an array. Compositions such as nucleic acid arrays produced by the methods are also disclosed.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.