公开(公告)号：US20230332247A1

公开(公告)日：2023-10-19

申请号：US18340501

申请日：2023-06-23

Applicant: 10x Genomics, Inc.

Inventor： Hardeep Pal Singh ,  David Sukovich ,  Seayar Mohabbat ,  Dongyao Li ,  Hanyoup Kim ,  Augusto Manuel Tentori

IPC: C12Q1/6886 ,  C12Q1/6834

CPC classification number: C12Q1/6886 ,  C12Q1/6834 ,  C12Q2600/158 ,  G01N2800/7028

Abstract: Provided herein are methods, compositions and systems for identifying spatial gene expression of analytes from glioblastoma derived tissue. The methods discloses herein include using templated ligation probe pairs to identify location of a disease proliferating region in a glioblastoma-derived sample by detecting analytes in the region and hybridizing a ligation product comprising the probe pairs to a capture probe on a spatial array.

公开(公告)号：US20250163501A1

公开(公告)日：2025-05-22

申请号：US18834805

申请日：2023-01-31

Applicant: 10x Genomics, Inc.

Inventor： Hardeep Pal Singh ,  David Sukovich ,  Augusto Manuel Tentori ,  Hanyoup Kim

IPC: C12Q1/6841 ,  C12Q1/37

Abstract: Provided herein are methods of analyzing an analyte in a fixed biological sample on a first substrate that has been stored for a long period of time, the method comprising: (a) hybridizing a first probe and a second probe to the analyte of the fixed biological sample; (b) coupling the first probe and the second probe, thereby generating a connected probe; (c) aligning the first substrate with a second substrate comprising an array, wherein the array comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises: (i) a spatial barcode and (ii) a capture domain; (d) when the biological sample is aligned with at least a portion of the array, (i) releasing the connected probe from the analyte and (ii) migrating the connected probe from the biological sample to the array; and (e) hybridizing the connected probe to the capture domain.

公开(公告)号：US20240218427A1

公开(公告)日：2024-07-04

申请号：US18399109

申请日：2023-12-28

Applicant: 10x Genomics, Inc.

Inventor： David Sukovich ,  Hardeep Pal Singh ,  Lauren Gutgesell ,  Ace George Santiago ,  Benton Veire ,  Anuj Patel

IPC: C12Q1/6823 ,  G01N33/58 ,  G01N33/68

CPC classification number: C12Q1/6823 ,  G01N33/58 ,  G01N33/6857 ,  G01N2458/10

Abstract: Provided herein are methods for spatial analysis of proteins and/or nucleic acids by capturing a capture handle sequence and/or a connected probe to a capture domain of a capture probe on a spatial array. In some instances, a reagent medium including a monovalent or divalent salt, ethylene carbonate, and/or glycerol is used in the disclosed methods.

公开(公告)号：US20230416850A1

公开(公告)日：2023-12-28

申请号：US18213088

申请日：2023-06-22

Applicant: 10x Genomics, Inc.

Inventor： Hardeep Pal Singh ,  Augusto Manuel Tentori

IPC: C12Q1/70 ,  C12Q1/6818

CPC classification number: C12Q1/701 ,  C12Q1/6818 ,  C12Q1/6806

Abstract: Provided herein are methods for capturing an exogenous probe and/or a capture handle sequence to a capture domain of a capture probe. Compositions, systems, and kits also are disclosed. In some instances, the methods include detecting an exogenous nucleic acid in a biological sample by hybridizing a first exogenous probe oligonucleotide and a second exogenous probe oligonucleotide to an exogenous nucleic acid in a biological sample on a first substrate, coupling the exogenous probe oligonucleotides, and capturing the coupled exogenous probe on a spatial array.