公开(公告)号：US20190233878A1

公开(公告)日：2019-08-01

申请号：US16374112

申请日：2019-04-03

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20240271183A1

公开(公告)日：2024-08-15

申请号：US18542404

申请日：2023-12-15

Applicant: 10X Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/00 ,  B01J13/18 ,  C08J3/075 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  B01J13/0065 ,  B01J13/18 ,  C08J3/075 ,  C08J2333/00 ,  C12Q1/6869

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20220364150A1

公开(公告)日：2022-11-17

申请号：US17861503

申请日：2022-07-11

Applicant: 10x Genomics, Inc.

Inventor： Joshua DELANEY ,  Shalini GOHIL ,  Christopher HINDSON ,  Adam LOWE ,  Andrew D. PRICE ,  Joseph Francis SHUGA

IPC: C12Q1/6806 ,  B01J13/18 ,  B01J13/00 ,  C08J3/075

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.