公开(公告)号：US20230220455A1

公开(公告)日：2023-07-13

申请号：US18179795

申请日：2023-03-07

申请人： 10x Genomics, Inc.

发明人： Christina Galonska ,  Marco Mignardi

IPC分类号： C12Q1/6837 ,  C12Q1/6855 ,  C12Q1/6841

CPC分类号： C12Q1/6837 ,  C12Q1/6855 ,  C12Q1/6841

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

公开(公告)号：US20240175080A1

公开(公告)日：2024-05-30

申请号：US18404317

申请日：2024-01-04

申请人： 10x Genomics, Inc.

发明人： Christina Galonska ,  Marco Mignardi

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

CPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

公开(公告)号：US11959130B2

公开(公告)日：2024-04-16

申请号：US18179795

申请日：2023-03-07

申请人： 10x Genomics, Inc.

发明人： Christina Galonska ,  Marco Mignardi

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

CPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

公开(公告)号：US20240018572A1

公开(公告)日：2024-01-18

申请号：US18032948

申请日：2021-10-21

申请人： 10x Genomics, Inc.

发明人： Marco Mignardi

IPC分类号： C12Q1/682 ,  C12Q1/6841

CPC分类号： C12Q1/682 ,  C12Q1/6841

摘要： Provided herein are methods of identifying a location of an analyte in a biological sample using padlock oligonucleotides and rolling circle amplification. For example, provided herein are methods for identifying a location of an analyte in a biological sample where (i) a padlock oligonucleotide hybridizes to an analyte and is ligated thereby creating a circularized padlock oligonucleotide, (ii) rolling circle amplification of the circularized padlock oligonucleotide results in generation of an amplified circularized padlock oligonucleotide, and (iii) a signal corresponding to the amplified circularized padlock oligonucleotide is detected and used to identify the location of the analyte in the biological sample. Also provided are methods for identifying whether a treatment of a biological sample facilitates the release and subsequent detection of an analyte from the biological sample using padlock oligonucleotides and rolling circle amplification.

公开(公告)号：US20230416821A1

公开(公告)日：2023-12-28

申请号：US18300814

申请日：2023-04-14

申请人： 10x Genomics, Inc.

发明人： Felice Alessio BAVA ,  Marco Mignardi

IPC分类号： C12Q1/6881 ,  C12Q1/6841

CPC分类号： C12Q1/6881 ,  C12Q1/6841

摘要： In some aspects disclosed herein are methods and compositions for detecting an analyte such as a target nucleic acid in a biological sample, said method comprising generating and analyzing a detectable signal associated with the target nucleic acid and a separate signal associated with a region of interest in the target nucleic acid.

公开(公告)号：US20240076723A1

公开(公告)日：2024-03-07

申请号：US18269634

申请日：2021-12-30

申请人： 10x Genomics, Inc.

发明人： Marco Mignardi

IPC分类号： C12Q1/6841 ,  C12Q1/6837

CPC分类号： C12Q1/6841 ,  C12Q1/6837 ,  C12Q2600/16

摘要： Provided herein are methods of determining a location of an analyte in a biological sample, and systems for performing these methods. In particular, the methods and systems comprise decreasing the binding events of an analyte from a biological sample to capture probes that surround a biological sample positioned on a substrate.

公开(公告)号：US11608520B2

公开(公告)日：2023-03-21

申请号：US17551980

申请日：2021-12-15

申请人： 10x Genomics, Inc.

发明人： Christina Galonska ,  Marco Mignardi

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.

公开(公告)号：US20230042817A1

公开(公告)日：2023-02-09

申请号：US17881086

申请日：2022-08-04

申请人： 10x Genomics, Inc.

发明人： Marco Mignardi

IPC分类号： C12Q1/6841 ,  C12N15/10

摘要： Provided herein are methods, compositions, and kits, for capturing analytes from an embedded biological sample and spatially barcoding the analytes with an array.

公开(公告)号：US20220106632A1

公开(公告)日：2022-04-07

申请号：US17551980

申请日：2021-12-15

申请人： 10x Genomics, Inc.

发明人： Christina Galonska ,  Marco Mignardi

IPC分类号： C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6855

摘要： Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample.