公开(公告)号：US20240368567A1

公开(公告)日：2024-11-07

申请号：US18744254

申请日：2024-06-14

Applicant: 10x Genomics, Inc.

Inventor： Derek Hunter VALLEJO ,  Sonya A. CLARK ,  Yufeng QIAN ,  Lorita BOGHOSPOR ,  Shankar SHASTRY

IPC: C12N9/12 ,  C12N9/10

Abstract: The disclosure provides recombinant reverse transcriptases comprising one or more DNA binding domains conjugated to an engineered reversed transcriptase that have been modified to exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both. The disclosure further provides compositions and kits comprising the recombinant reverse transcriptase enzymes and methods of producing, amplifying or sequencing nucleic acid molecules using these fusion reverse transcriptase enzymes.

公开(公告)号：US20240174990A1

公开(公告)日：2024-05-30

申请号：US18440901

申请日：2024-02-13

Applicant: 10x Genomics, Inc.

Inventor： Sonya A. CLARK ,  Derek Hunter VALLEJO ,  Carolina CUELLAR ,  Katherine PFEIFFER

IPC: C12N9/12

CPC classification number: C12N9/1241 ,  C12Y207/07049

Abstract: The application provides compositions including engineered reverse transcriptases with at least one altered reverse-transcriptase related activity. The engineered reverse transcriptases or reverse transcription enzymes unexpectedly exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both.

公开(公告)号：US20220403350A1

公开(公告)日：2022-12-22

申请号：US17837497

申请日：2022-06-10

Applicant: 10X Genomics, Inc.

Inventor： Sonya A. CLARK ,  Derek Hunter Vallejo ,  Carolina Cuellar ,  Katherine Pfeiffer

IPC: C12N9/12

Abstract: The application provides compositions including engineered reverse transcriptases with at least one altered reverse-transcriptase related activity. The engineered reverse transcriptases or reverse transcription enzymes unexpectedly exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both.