公开(公告)号：US20240209330A1

公开(公告)日：2024-06-27

申请号：US18545941

申请日：2023-12-19

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY ,  Robert Henley

IPC: C12N9/12 ,  C07K14/47 ,  C12N15/63 ,  C12Q1/6844

CPC classification number: C12N9/1252 ,  C07K14/47 ,  C12N15/63 ,  C12Q1/6844 ,  C07K2319/21 ,  C12Y207/07007

Abstract: The present disclosure relates in some aspects to compositions and methods of using Phi29 polymerase fusion proteins. In some aspects, the compositions and methods provided herein address certain limitations associated with the Phi29 polymerase. In some aspects, the composition and methods disclosed herein provide more robust nucleic acid amplification for improved in vitro and in situ analysis.

公开(公告)号：US20230374573A1

公开(公告)日：2023-11-23

申请号：US18127582

申请日：2023-03-28

Applicant: 10x Genomics, Inc.

Inventor： Yufeng QIAN ,  Shankar SHASTRY

IPC: C12Q1/6844 ,  C12N9/22 ,  C12Q1/44

CPC classification number: C12Q1/6844 ,  C12N9/22 ,  C12Y301/26004 ,  C12Q1/44

Abstract: The present disclosure relates in some aspects to methods for analyzing a target RNA in a biological sample, such as detection of a sequence of interest in an RNA. In some aspects, provided herein are methods of using an RNase such as RNase H to provide in situ RNA detection methods having high sensitivity, efficiency, and/or specificity.

公开(公告)号：US20230374475A1

公开(公告)日：2023-11-23

申请号：US18319864

申请日：2023-05-18

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY

IPC: C12N9/12 ,  C12N15/63 ,  C12N15/10

CPC classification number: C12N9/1241 ,  C12Y207/07049 ,  C12N15/63 ,  C12N15/102

Abstract: The present disclosure relates generally to engineered nucleic acid processing enzymes and derivatives thereof, compositions and kits comprising the same; and methods of generating and using the same.

公开(公告)号：US20240368567A1

公开(公告)日：2024-11-07

申请号：US18744254

申请日：2024-06-14

Applicant: 10x Genomics, Inc.

Inventor： Derek Hunter VALLEJO ,  Sonya A. CLARK ,  Yufeng QIAN ,  Lorita BOGHOSPOR ,  Shankar SHASTRY

IPC: C12N9/12 ,  C12N9/10

Abstract: The disclosure provides recombinant reverse transcriptases comprising one or more DNA binding domains conjugated to an engineered reversed transcriptase that have been modified to exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both. The disclosure further provides compositions and kits comprising the recombinant reverse transcriptase enzymes and methods of producing, amplifying or sequencing nucleic acid molecules using these fusion reverse transcriptase enzymes.

公开(公告)号：US20240084373A1

公开(公告)日：2024-03-14

申请号：US18450212

申请日：2023-08-15

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY

IPC: C12Q1/686 ,  C12N9/12

CPC classification number: C12Q1/686 ,  C12N9/1247

Abstract: The present disclosure relates in some aspects to recombinant Bacillus phage AP50 polymerases, modified AP50 polymerases, and compositions thereof. Also provided are methods of using the recombinant and/or modified AP50 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.

公开(公告)号：US20240150745A1

公开(公告)日：2024-05-09

申请号：US18481673

申请日：2023-10-05

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY ,  Javelin C. CHI ,  Paul Eugene LUND

IPC: C12N9/00 ,  C07K14/245 ,  C12Q1/6827

CPC classification number: C12N9/93 ,  C07K14/245 ,  C12Q1/6827 ,  C07K2319/21 ,  C07K2319/60 ,  C07K2319/80

Abstract: A recombinant ligase composition including a recombinantly-produced ligase isolated from Acanthocystis turfacea chlorella virus 1 (ATCV-1) fused to at least one polynucleotide-binding polypeptide is provided. Compositions containing the recombinant ligase composition, vectors encoding the recombinant ligase composition, and methods of making and using the recombinant ligase composition are also provided.

公开(公告)号：US20240425913A1

公开(公告)日：2024-12-26

申请号：US18821593

申请日：2024-08-30

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY

IPC: C12Q1/686 ,  C12N9/12

Abstract: The present disclosure relates in some aspects to recombinant Bacillus phage AP50 polymerases, modified AP50 polymerases, and compositions thereof. Also provided are methods of using the recombinant and/or modified AP50 polymerases for nucleic acid amplification (e.g., rolling circle amplification). In some aspects, the compositions and methods disclosed herein provide more robust amplification (e.g., RCA) reactions for improved in vitro and in situ analysis.

公开(公告)号：US20240174991A1

公开(公告)日：2024-05-30

申请号：US18384537

申请日：2023-10-27

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY ,  Derek Hunter VALLEJO

IPC: C12N9/12 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C07K2319/80

Abstract: The application provides compositions including engineered fusion reverse transcriptases with at least one altered reverse-transcriptase related activity. The engineered fusion reverse transcriptases or reverse transcription enzymes unexpectedly exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both.

公开(公告)号：US20240043910A1

公开(公告)日：2024-02-08

申请号：US18362074

申请日：2023-07-31

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY

IPC: C12Q1/682 ,  C12Q1/6837

CPC classification number: C12Q1/682 ,  C12Q1/6837 ,  C12Q2600/16

Abstract: The present disclosure relates in some aspects to methods and compositions for analyzing a target nucleic acid in a biological sample. In some aspects, the methods disclosed herein promote hybridization of polynucleotides to target nucleic acids and/or splints for enhanced ligation efficiency and/or enhanced hybridization efficiency in situ in a biological sample. In some aspects, the presence, amount, and/or identity of a target nucleic acid is analyzed in situ. Also provided are compositions and kits for use in accordance with the methods.

公开(公告)号：US20230323430A1

公开(公告)日：2023-10-12

申请号：US18132298

申请日：2023-04-07

Applicant: 10x Genomics, Inc.

Inventor： Shankar SHASTRY

IPC: C12Q1/682 ,  C12Q1/6874 ,  C12Q1/6855

CPC classification number: C12Q1/682 ,  C12Q1/6874 ,  C12Q1/6855

Abstract: The present disclosure relates in some aspects to methods for analyzing a target nucleic acid in a biological sample. In some aspects, provided herein are methods and compositions for improving the specificity of ligation in situ in biological samples, as well as in single cell analysis and spatial applications of RNA templated ligation reactions.