公开(公告)号：US20250147036A1

公开(公告)日：2025-05-08

申请号：US18708833

申请日：2022-11-09

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Geoffrey P. MCDERMOTT ,  David Michael PATTERSON ,  Camilla ENGBLOM ,  Kim THRANE ,  Jeffrey MOLD ,  Jonas FRISEN ,  Joakim LUNDBERG ,  Qirong LIN ,  Caroline Julie GALLANT ,  Marlon STOECKIUS ,  Katherine PFEIFFER ,  Zachary BENT ,  Christina GALONSKA ,  Ariel ROYALL

IPC: G01N33/68 ,  C12N15/10

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

公开(公告)号：US20230340565A1

公开(公告)日：2023-10-26

申请号：US18097989

申请日：2023-01-17

Applicant: 10X GENOMICS, INC.

Inventor： Zahra Kamila BELHOCINE ,  Josephine Lee ,  Francesca MESCHI ,  Luz MONTESCLAROS ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Jerald SAPIDA ,  David SUKOVICH ,  Tobias Daniel WHEELER ,  Yifeng YIN

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

公开(公告)号：US20240174990A1

公开(公告)日：2024-05-30

申请号：US18440901

申请日：2024-02-13

Applicant: 10x Genomics, Inc.

Inventor： Sonya A. CLARK ,  Derek Hunter VALLEJO ,  Carolina CUELLAR ,  Katherine PFEIFFER

IPC: C12N9/12

CPC classification number: C12N9/1241 ,  C12Y207/07049

Abstract: The application provides compositions including engineered reverse transcriptases with at least one altered reverse-transcriptase related activity. The engineered reverse transcriptases or reverse transcription enzymes unexpectedly exhibit one or more altered reverse transcriptase related activities such as but not limited to altered template switching efficiency, altered transcription efficiency or both.

公开(公告)号：US20220106641A1

公开(公告)日：2022-04-07

申请号：US17473760

申请日：2021-09-13

Applicant: 10X Genomics, Inc.

Inventor： Phillip BELGRADER ,  Zachary BENT ,  Rajiv BHARADWAJ ,  Vijay Kumar Sreenivasa GOPALAN ,  Josephine HARADA ,  Christopher HINDSON ,  Mohammad Rahimi LENJI ,  Michael Ybarra LUCERO ,  Geoffrey McDERMOTT ,  Elliott MEER ,  Tarjei Sigurd MIKKELSEN ,  Christopher Joachim O'KEEFFE ,  Katherine PFEIFFER ,  Andrew D. PRICE ,  Paul RYVKIN ,  Serge SAXONOV ,  John R. STUELPNAGEL ,  Jessica Michele TERRY ,  Tobias Daniel WHEELER ,  Indira WU ,  Solongo Batjargal ZIRALDO ,  Stephane Claude BOUTET ,  Sarah TAYLOR ,  Niranjan SRINIVAS

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/683

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20250146056A1

公开(公告)日：2025-05-08

申请号：US18945336

申请日：2024-11-12

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Zahra Kamila BELHOCINE

IPC: C12Q1/6813 ,  C12Q1/6876

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. The methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240309448A1

公开(公告)日：2024-09-19

申请号：US18737828

申请日：2024-06-07

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Andrew Scott KOHLWAY ,  Andrew John HILL ,  Paul Eugene LUND

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240254552A1

公开(公告)日：2024-08-01

申请号：US18412111

申请日：2024-01-12

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20200216897A1

公开(公告)日：2020-07-09

申请号：US16773750

申请日：2020-01-27

Applicant: 10X GENOMICS, INC.

Inventor： Josephine HARADA ,  Tarjei Sigurd MIKKELSEN ,  Katherine PFEIFFER ,  Serge SAXONOV ,  John R. STUELPNAGEL

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20240247303A1

公开(公告)日：2024-07-25

申请号：US18392743

申请日：2023-12-21

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Zahra Kamila BELHOCINE

IPC: C12Q1/6813 ,  C12Q1/6876

CPC classification number: C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. The methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240190957A1

公开(公告)日：2024-06-13

申请号：US18452127

申请日：2023-08-18

Applicant: 10x Genomics, Inc.

Inventor： Wyatt James MCDONNELL ,  Michael John Terry STUBBINGTON ,  Katherine PFEIFFER

IPC: C07K16/28 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12N15/85

CPC classification number: C07K16/28 ,  C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12N15/85 ,  C07K2317/622 ,  C12N2310/20 ,  C12Y305/04005

Abstract: The present disclosure generally relates to compositions (e.g., nucleic acid constructs, vectors, plasmids, engineered cells, etc.) and methods using the same for selecting and/or making an antigen-binding molecule of interest (e.g., an immune receptor, an antibody or functional fragment thereof) with improved affinity to a target antigen.