公开(公告)号：US20180009877A1

公开(公告)日：2018-01-11

申请号：US15547523

申请日：2016-02-23

Applicant: Academia Sinica

Inventor： An-Suei YANG ,  Hong-Sen CHEN ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Shin-Chen HOU ,  Chung-Ming YU ,  Chi-Kai YANG ,  Yi-Kai CHIU

IPC: C07K16/00 ,  C12N15/10 ,  C07K16/22 ,  C07K16/32

CPC classification number: C07K16/005 ,  C07K16/1018 ,  C07K16/18 ,  C07K16/22 ,  C07K16/245 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2863 ,  C07K16/2887 ,  C07K16/32 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/77 ,  C07K2317/92 ,  C12N15/1037

Abstract: Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, that comprised a plurality of phage-displayed scFvs characterized with (1) a specific CS combination; (2) a specific distribution of aromatic residues in each CDR; and (3) a specific sequence in each CDR. The present scFv library could be used to efficiently produce different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

公开(公告)号：US20190263893A1

公开(公告)日：2019-08-29

申请号：US16409994

申请日：2019-05-13

Applicant: Academia Sinica

Inventor： An-Suei YANG ,  Hong-Sen CHEN ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Shin-Chen HOU ,  Chung-Ming YU ,  Chi-Kai YANG ,  Yi-Kai CHIU

IPC: C07K16/00 ,  C12N15/10 ,  C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/24 ,  C07K16/32 ,  C07K16/10

Abstract: Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, that comprised a plurality of phage-displayed scFvs characterized with (1) a specific CS combination; (2) a specific distribution of aromatic residues in each CDR; and (3) a specific sequence in each CDR. The present scFv library could be used to efficiently produce different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

公开(公告)号：US20210070843A1

公开(公告)日：2021-03-11

申请号：US16757460

申请日：2018-10-19

Applicant: ACADEMIA SINICA

Inventor： An-Suei YANG ,  Ing-Chien CHEN ,  Yi-Kai CHIU ,  Chung-Ming YU ,  Cheng-Chung LEE ,  Chao-Ping TUNG ,  Yueh-Liang TSOU ,  Yi-Jen HUANG ,  Chia-Lung LIN ,  Hong-Sen CHEN ,  Hwei-Jiung WANG

IPC: C07K16/10

Abstract: Disclosed herein are methods for high-throughput screening of a virus-specific neutralizing antibody. According to certain embodiments of the present disclosure, the virus is an influenza virus. Also disclosed herein are the antibodies selected by the high-throughput screening method, and the uses thereof in the prophylaxis and/or treatment of viral infection.

公开(公告)号：US20190040151A1

公开(公告)日：2019-02-07

申请号：US16166220

申请日：2018-10-22

Applicant: Academia Sinica

Inventor： An-Suei YANG ,  Wei-Ying KUO ,  Hung-Ju HSU ,  Hong-Sen CHEN ,  Yu-Chi CHOU ,  Yueh-Liang TSOU ,  Hung-Pin PENG ,  Jhih-Wei JIAN ,  Chung-Ming YU ,  Yi-Kai CHIU ,  Ing-Chein CHEN ,  Chao-Ping TUNG ,  Michael HSIAO ,  Hwei-Jiung WANG

IPC: C07K16/30 ,  A61K47/68 ,  A61K39/395 ,  A61P35/00

CPC classification number: C07K16/3015 ,  A61K39/39558 ,  A61K47/6829 ,  A61K47/6855 ,  A61K47/6889 ,  A61K2039/505 ,  A61P35/00 ,  C07K16/32 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77 ,  C07K2317/92 ,  C07K2317/94

Abstract: Disclosed herein is an immunoconjugate comprising an antibody, a functional motif, and a linker connecting the functional motif to the antibody. According to embodiments of the present disclosure, the antibody may recognize tumor-associated antigens (TAAs), and serves as a targeting module for delivering the functional motif connected therewith to the tumor cells thereby inhibiting tumor growth or detecting the distribution of tumor cells. Also disclosed herein are methods of treating cancers and methods of diagnosing cancers by use of the present immunoconjugate.

公开(公告)号：US20170355750A1

公开(公告)日：2017-12-14

申请号：US15619835

申请日：2017-06-12

Applicant: Academia Sinica

Inventor： An-Suei YANG ,  Hong-Sen CHEN ,  Chung-Ming YU ,  Shin-Chen HOU ,  Wei-Ying KUO ,  Yi-Kai CHIU ,  Yueh-Liang TSOU ,  Hung-Ju HSU ,  Hwei-Jiung WANG ,  Shih-Hsien CHUANG ,  Chao-Pin LEE

IPC: C07K16/00 ,  C07K16/32 ,  C07K19/00 ,  A61K39/00

Abstract: Disclosed herein are methods for high-throughput screening of a functional antibody fragment for an immunoconjugate that targets a protein antigen. The method combines a phage-displayed synthetic antibody library and high-throughput cytotoxicity screening of non-covalently assembled immunotoxins or cytotoxic drug to identify highly functional synthetic antibody fragments for delivering toxin payloads.