公开(公告)号：US20240277865A1

公开(公告)日：2024-08-22

申请号：US18567765

申请日：2022-06-08

申请人： Academia Sinica

发明人： An-Suei YANG ,  Hung-Ju HSU ,  Chao-Ping TUNG ,  Chung-Ming YU ,  Chi-Yung CHEN ,  Hong-Sen CHEN ,  Yu-Chuan HUANG ,  Pei-Hsun TSAI ,  Szu-Yu LIN ,  Hung-Pin PENG

IPC分类号： A61K47/68 ,  A61K39/00 ,  A61P35/00 ,  C07K16/00 ,  C07K16/30

CPC分类号： A61K47/6851 ,  A61K47/68031 ,  A61K47/6829 ,  A61P35/00 ,  C07K16/005 ,  C07K16/30 ,  A61K2039/505 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/73

摘要： Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, which comprises a plurality of phage-displayed scFvs characterized with a specific sequence in each CDR. The present phage-displayed scFv library is useful in selecting an antibody fragment exhibiting a binding affinity and specificity to mesothelin (MSLN). Also disclosed herein are a recombinant antibody specific to MSLN, an immunoconjugate comprising the recombinant antibody, and uses thereof in treating cancers.

公开(公告)号：US20210070843A1

公开(公告)日：2021-03-11

申请号：US16757460

申请日：2018-10-19

申请人： ACADEMIA SINICA

发明人： An-Suei YANG ,  Ing-Chien CHEN ,  Yi-Kai CHIU ,  Chung-Ming YU ,  Cheng-Chung LEE ,  Chao-Ping TUNG ,  Yueh-Liang TSOU ,  Yi-Jen HUANG ,  Chia-Lung LIN ,  Hong-Sen CHEN ,  Hwei-Jiung WANG

IPC分类号： C07K16/10

摘要： Disclosed herein are methods for high-throughput screening of a virus-specific neutralizing antibody. According to certain embodiments of the present disclosure, the virus is an influenza virus. Also disclosed herein are the antibodies selected by the high-throughput screening method, and the uses thereof in the prophylaxis and/or treatment of viral infection.

公开(公告)号：US20180009877A1

公开(公告)日：2018-01-11

申请号：US15547523

申请日：2016-02-23

申请人： Academia Sinica

发明人： An-Suei YANG ,  Hong-Sen CHEN ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Shin-Chen HOU ,  Chung-Ming YU ,  Chi-Kai YANG ,  Yi-Kai CHIU

IPC分类号： C07K16/00 ,  C12N15/10 ,  C07K16/22 ,  C07K16/32

CPC分类号： C07K16/005 ,  C07K16/1018 ,  C07K16/18 ,  C07K16/22 ,  C07K16/245 ,  C07K16/2803 ,  C07K16/2827 ,  C07K16/2863 ,  C07K16/2887 ,  C07K16/32 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/77 ,  C07K2317/92 ,  C12N15/1037

摘要： Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, that comprised a plurality of phage-displayed scFvs characterized with (1) a specific CS combination; (2) a specific distribution of aromatic residues in each CDR; and (3) a specific sequence in each CDR. The present scFv library could be used to efficiently produce different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

公开(公告)号：US20170044239A1

公开(公告)日：2017-02-16

申请号：US15232819

申请日：2016-08-10

申请人： Academia Sinica

发明人： An-Suei YANG ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Chung-Ming YU

IPC分类号： C07K16/00 ,  C12N15/10 ,  C12N15/85 ,  C07K16/10

CPC分类号： C07K16/005 ,  C07K16/1018 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92 ,  C12N15/1037

摘要： Disclosed herein are phage-displayed single-chain variable fragment (scFv) libraries, which comprised a plurality of scFvs with a specific sequence in each CDR. The present scFv libraries could be used to efficiently produce different antibodies with high binding affinity to H1 hemagglutinin of influenza virus. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

摘要翻译： 本文公开了噬菌体展示的单链可变片段（scFv）文库，其包含在每个CDR中具有特定序列的多个scFv。 目前的scFv文库可用于有效地产生具有高流感病毒H1血凝素的高结合亲和力的不同抗体。 因此，本公开提供了根据实验研究和/或临床应用中的需要迅速产生不同抗原特异性抗体的潜在手段。

公开(公告)号：US20230212554A1

公开(公告)日：2023-07-06

申请号：US18008753

申请日：2021-06-07

申请人： An-Suei YU ,  Academia Sinica

发明人： An-Suei YANG ,  Chung-Ming YU ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Hung-Pin PENG

IPC分类号： C12N15/10 ,  G01N33/569 ,  C07K16/10

CPC分类号： C12N15/1037 ,  G01N33/56983 ,  C07K16/1018 ,  G01N2333/11 ,  G01N2470/04 ,  C07K2317/622 ,  C07K2317/92

摘要： Disclosed herein are methods for selecting an antibody fragment specific to an influenza virus. According to certain embodiments of the present disclosure, the influenza virus may be influenza virus type A (IAV) or influenza virus type B (IBV). Also disclosed herein are the selected antibodies, recombinant antibody produced from the selected antibodies, and the uses thereof in the diagnosis of influenza virus infection.

公开(公告)号：US20190263893A1

公开(公告)日：2019-08-29

申请号：US16409994

申请日：2019-05-13

申请人： Academia Sinica

发明人： An-Suei YANG ,  Hong-Sen CHEN ,  Ing-Chien CHEN ,  Chao-Ping TUNG ,  Shin-Chen HOU ,  Chung-Ming YU ,  Chi-Kai YANG ,  Yi-Kai CHIU

IPC分类号： C07K16/00 ,  C12N15/10 ,  C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/24 ,  C07K16/32 ,  C07K16/10

摘要： Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, that comprised a plurality of phage-displayed scFvs characterized with (1) a specific CS combination; (2) a specific distribution of aromatic residues in each CDR; and (3) a specific sequence in each CDR. The present scFv library could be used to efficiently produce different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.

公开(公告)号：US20230168249A1

公开(公告)日：2023-06-01

申请号：US17922590

申请日：2021-05-04

申请人： Academia Sinica

发明人： An-Suei YANG ,  Chao-Ping TUNG ,  Chung-Ming YU ,  Chi-Yung CHEN ,  Yu-Chuan HUANG ,  Pei-Hsun TSAI ,  Szu-Yu LIN ,  Hung-Ju HSU ,  Hung-Pin PENG ,  Fei-Hung HUNG

IPC分类号： G01N33/569 ,  C07K16/10

CPC分类号： G01N33/56983 ,  C07K16/10 ,  C07K2317/622 ,  C07K2317/92 ,  G01N2333/165 ,  G01N2470/04 ,  G01N2469/10

摘要： Disclosed herein are recombinant antibodies or the fragment thereof for detecting severe acute respiratory syndrome coronavirus (SARS-CoV). According to some embodiments, the SARS-CoV is SARS-CoV-1. According to some alternative embodiments, the SARS-CoV is SARS-CoV-2. Also disclosed herein are a kit comprising the recombinant antibodies, and a method for diagnosing the infection of SARS-CoV by using the recombinant antibody or the kit.

公开(公告)号：US20190040151A1

公开(公告)日：2019-02-07

申请号：US16166220

申请日：2018-10-22

申请人： Academia Sinica

发明人： An-Suei YANG ,  Wei-Ying KUO ,  Hung-Ju HSU ,  Hong-Sen CHEN ,  Yu-Chi CHOU ,  Yueh-Liang TSOU ,  Hung-Pin PENG ,  Jhih-Wei JIAN ,  Chung-Ming YU ,  Yi-Kai CHIU ,  Ing-Chein CHEN ,  Chao-Ping TUNG ,  Michael HSIAO ,  Hwei-Jiung WANG

IPC分类号： C07K16/30 ,  A61K47/68 ,  A61K39/395 ,  A61P35/00

CPC分类号： C07K16/3015 ,  A61K39/39558 ,  A61K47/6829 ,  A61K47/6855 ,  A61K47/6889 ,  A61K2039/505 ,  A61P35/00 ,  C07K16/32 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/77 ,  C07K2317/92 ,  C07K2317/94

摘要： Disclosed herein is an immunoconjugate comprising an antibody, a functional motif, and a linker connecting the functional motif to the antibody. According to embodiments of the present disclosure, the antibody may recognize tumor-associated antigens (TAAs), and serves as a targeting module for delivering the functional motif connected therewith to the tumor cells thereby inhibiting tumor growth or detecting the distribution of tumor cells. Also disclosed herein are methods of treating cancers and methods of diagnosing cancers by use of the present immunoconjugate.