公开(公告)号：US10689428B2

公开(公告)日：2020-06-23

申请号：US15865950

申请日：2018-01-09

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Bruno Reversade

IPC: C07K14/575 ,  G01N33/74 ,  C07K16/26 ,  C07K7/00 ,  C07K14/46 ,  C12N15/113 ,  A61K38/00

Abstract: We describe an ELABELA polypeptide comprising a sequence CXXXRCXXXHSRVPFP (SEQ ID NO: 1), in which X signifies an amino acid residue, such as a sequence selected from the group consisting of: SEQ ID NO: 2 to SEQ ID NO: 18, preferably CLQRRCMPLHSRVPFP (SEQ ID NO: 2), or a fragment, homologue, variant or derivative thereof, which polypeptide is capable of maintaining self-renewal and/or pluripotency of a stem cell.

公开(公告)号：US09862986B2

公开(公告)日：2018-01-09

申请号：US14583083

申请日：2014-12-24

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Bruno Reversade ,  Stefan Mundlos

IPC: C12Q1/26 ,  C12N9/06 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/573 ,  A61K38/00

CPC classification number: C12Q1/26 ,  A61K38/00 ,  C12N9/0028 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/3233 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Y105/01002 ,  G01N33/5088 ,  G01N33/573 ,  G01N2333/90661 ,  G01N2800/20

Abstract: The invention relates to muteins of the pyrroline-5-carboxylate reductase 1 (PYCR1), to nucleic acid molecules comprising a nucleotide sequence encoding such muteins, to methods of determining in a subject a predisposition of having an age related disorder associated with PYCR1, to methods of identifying a compound capable of modifying the expression of PYCR1, and methods of treating a subject having an age-related disorder associated with PYCR1. The invention further relates to a genetically modified animal and a method of modifying the expression of the PYCR1 gene in an animal.

公开(公告)号：US09902761B2

公开(公告)日：2018-02-27

申请号：US15101304

申请日：2014-12-03

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Bruno Reversade

IPC: C07K14/575 ,  G01N33/74 ,  C07K16/26 ,  C07K7/00 ,  C07K14/46 ,  C12N15/113 ,  A61K38/00

CPC classification number: C07K14/575 ,  A61K38/00 ,  C07K7/00 ,  C07K14/46 ,  C07K16/26 ,  C07K2317/30 ,  C07K2317/34 ,  C12N15/113 ,  C12N2310/14 ,  G01N33/74 ,  G01N2333/575

Abstract: We describe an ELABELA polypeptide comprising a sequence CXXXRCXXXHSRVPFP (SEQ ID NO: 1), in which X signifies an amino acid residue, such as a sequence selected from the group consisting of SEQ ID NO: 2 to SEQ ID NO: 18, preferably CLQRRCMPLHSRVPFP (SEQ ID NO: 2), or a fragment, homologue, variant or derivative thereof, which polypeptide is capable of maintaining self-renewal and/or pluripotency of a stem cell.

公开(公告)号：US09309314B2

公开(公告)日：2016-04-12

申请号：US14496600

申请日：2014-09-25

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH (A*STAR)

Inventor： Bruno Reversade

IPC: G01N33/53 ,  C07K16/26 ,  G01N33/74 ,  C07K14/575 ,  C07K7/00

CPC classification number: C07K14/575 ,  A61K38/00 ,  C07K7/00 ,  C07K14/46 ,  C07K16/26 ,  C07K2317/30 ,  C07K2317/34 ,  C12N15/113 ,  C12N2310/14 ,  G01N33/74 ,  G01N2333/575

Abstract: We describe an ELABELA polypeptide comprising a sequence CXXXRCXXXHSRVPFP (SEQ ID NO: 1), in which X signifies an amino acid residue, such as a sequence selected from the group consisting of: SEQ ID NO: 2 to SEQ ID NO: 18, preferably CLQRRCMPLHSRVPFP (SEQ ID NO: 2), or a fragment, homolog, variant or derivative thereof, which polypeptide is capable of maintaining self-renewal and/or pluripotency of a stem cell.

Abstract translation: 我们描述了包含序列CXXXRCXXXHSRVPFP（SEQ ID NO：1）的ELABELA多肽，其中X表示氨基酸残基，例如选自SEQ ID NO：2至SEQ ID NO：18的序列，优选 CLQRRCMPLHSRVPFP（SEQ ID NO：2）或其片段，同系物，变体或衍生物，其能够维持干细胞的自我更新和/或多能性。