公开(公告)号：US11873508B2

公开(公告)日：2024-01-16

申请号：US16473266

申请日：2018-01-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Leng Leng Chng ,  Daniele Zink ,  Aysha Farwin

IPC: C12N5/00 ,  C08F293/00 ,  C08L53/00 ,  C12N5/0775

CPC classification number: C12N5/0075 ,  C08F293/005 ,  C08L53/005 ,  C12N5/0662 ,  C08F2438/00 ,  C08F2810/20 ,  C08F2810/50 ,  C08L2207/53 ,  C08L2312/00 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2531/00 ,  C12N2533/30 ,  C12N2539/10

Abstract: There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.

公开(公告)号：US20200149001A1

公开(公告)日：2020-05-14

申请号：US16473266

申请日：2018-01-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. Ying ,  Leng Leng Chng ,  Daniele Zink ,  Aysha Farwin

IPC: C12N5/00 ,  C08F293/00 ,  C08L53/00 ,  C12N5/0775

Abstract: There is provided a polymeric microsphere comprising a thermally responsive monomer crosslinked with a functional group monomer, wherein the functional group monomer comprises at least one of a carboxylic acid functional group or an amine functional group. The thermally responsive monomer is preferably N-isopropylacrylamide (NIPAM), and the microspheres preferably comprise a coating of polymerized catecholamines (e.g. DOPA). There is also provided a method of preparing the polymeric microsphere and uses of the polymeric microsphere in culturing, harvesting, or expanding stem cells or stromal cells. Preferably, the cells, e.g. hMSCs (human mesenchymal stem/stromal cells), are expanded or harvested in serum-free and xeno-free medium.