公开(公告)号：US09976126B2

公开(公告)日：2018-05-22

申请号：US14862323

申请日：2015-09-23

Applicant: AJINOMOTO CO., INC.

Inventor： Yuya Kodama ,  Wataru Hoshino ,  Kazutoshi Takahashi ,  Moemi Ito

IPC: C12N9/00 ,  C12Q1/26 ,  C12P7/42 ,  C12P7/40 ,  C12Q1/00 ,  G01N27/327 ,  C12N9/06

CPC classification number: C12N9/0022 ,  C12P7/40 ,  C12P7/42 ,  C12Q1/005 ,  C12Q1/26 ,  C12Y104/03019 ,  G01N27/3271 ,  G01N2333/90638

Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.

公开(公告)号：US20160002610A1

公开(公告)日：2016-01-07

申请号：US14862323

申请日：2015-09-23

Applicant: AJINOMOTO CO., INC.

Inventor： Yuya Kodama ,  Wataru Hoshino ,  Kazutoshi Takahashi ,  Moemi Ito

IPC: C12N9/06 ,  G01N27/327 ,  C12Q1/00 ,  C12Q1/26 ,  C12P7/40

CPC classification number: C12N9/0022 ,  C12P7/40 ,  C12P7/42 ,  C12Q1/005 ,  C12Q1/26 ,  C12Y104/03019 ,  G01N27/3271 ,  G01N2333/90638

Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.

Abstract translation: 本发明提供了一种新的酶和使用酶测定甘氨酸浓度的方法。 具体地说，本发明提供一种其中至少一个氨基酸残基被突变以改善与甘氨酸测量相关的甘氨酸氧化酶的性质的酶（例如甘氨酸的甘氨酸氧化酶的活性，甘氨酸的热稳定性 氧化酶和甘氨酸氧化酶对甘氨酸的底物特异性）; 以及分析甘氨酸的方法，其包括使用所述修饰酶测定测试样品中所含的甘氨酸; 等等。