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公开(公告)号：US09976126B2
公开(公告)日：2018-05-22
申请号：US14862323
申请日：2015-09-23
Applicant: AJINOMOTO CO., INC.
Inventor： Yuya Kodama , Wataru Hoshino , Kazutoshi Takahashi , Moemi Ito
CPC classification number: C12N9/0022 , C12P7/40 , C12P7/42 , C12Q1/005 , C12Q1/26 , C12Y104/03019 , G01N27/3271 , G01N2333/90638
Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
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公开(公告)号：US20160362662A1
公开(公告)日：2016-12-15
申请号：US15250056
申请日：2016-08-29
Applicant: AJINOMOTO CO., INC.
Inventor： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
CPC classification number: C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
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Patent Agency Ranking
公开(公告)号：US20150010936A1
公开(公告)日：2015-01-08
申请号：US14491501
申请日：2014-09-19
Applicant: AJINOMOTO CO., INC.
Inventor： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
CPC classification number: C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
Abstract translation: 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。
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公开(公告)号：US09453206B2
公开(公告)日：2016-09-27
申请号：US15133599
申请日：2016-04-20
Applicant: AJINOMOTO CO., INC.
Inventor： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
CPC classification number: C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
Abstract translation: 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。
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公开(公告)号：US09347047B2
公开(公告)日：2016-05-24
申请号：US14491501
申请日：2014-09-19
Applicant: AJINOMOTO CO., INC.
Inventor： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
CPC classification number: C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
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公开(公告)号：US20160222359A1
公开(公告)日：2016-08-04
申请号：US15133599
申请日：2016-04-20
Applicant: AJINOMOTO CO., INC.
Inventor： Wataru Hoshino , Yuya Kodama , Toshimi Mizukoshi , Uno Tagami
CPC classification number: C12N9/0016 , C07K2319/02 , C12P13/06 , C12P13/08 , C12Q1/32 , C12Y104/01009 , G01N33/6812 , G01N2333/90616
Abstract: The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.
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公开(公告)号：US20160002610A1
公开(公告)日：2016-01-07
申请号：US14862323
申请日：2015-09-23
Applicant: AJINOMOTO CO., INC.
Inventor： Yuya Kodama , Wataru Hoshino , Kazutoshi Takahashi , Moemi Ito
IPC: C12N9/06 , G01N27/327 , C12Q1/00 , C12Q1/26 , C12P7/40
CPC classification number: C12N9/0022 , C12P7/40 , C12P7/42 , C12Q1/005 , C12Q1/26 , C12Y104/03019 , G01N27/3271 , G01N2333/90638
Abstract: The present invention provides a novel enzyme and methods of using the enzyme for measuring glycine concentration. Specifically, the present invention provides an enzyme in which at least one amino acid residue is mutated so as to improve a property of a glycine oxidase which is associated with the measurement of glycine (e.g., activity of glycine oxidase for glycine, thermal stability of glycine oxidase, and substrate specificity of glycine oxidase for glycine,); and a method of analyzing glycine, that includes measuring glycine contained in a test sample using the modified enzyme; and the like.
Abstract translation: 本发明提供了一种新的酶和使用酶测定甘氨酸浓度的方法。 具体地说，本发明提供一种其中至少一个氨基酸残基被突变以改善与甘氨酸测量相关的甘氨酸氧化酶的性质的酶（例如甘氨酸的甘氨酸氧化酶的活性，甘氨酸的热稳定性 氧化酶和甘氨酸氧化酶对甘氨酸的底物特异性）; 以及分析甘氨酸的方法，其包括使用所述修饰酶测定测试样品中所含的甘氨酸; 等等。
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