公开(公告)号：US20240003056A1

公开(公告)日：2024-01-04

申请号：US18354037

申请日：2023-07-18

Applicant: ALLOGENE THERAPEUTICS, INC.

Inventor： Thomas Charles PERTEL ,  Barbra Johnson SASU

IPC: C40B40/10 ,  C07K16/40 ,  C12N15/63

CPC classification number: C40B40/10 ,  C07K16/40 ,  C12N15/63 ,  C07K2317/565

Abstract: The present disclosure provides, among other things, antibodies for detecting TALENs and/or FokI nucleases in a sample and methods of using the same.

公开(公告)号：US20200339944A1

公开(公告)日：2020-10-29

申请号：US16857394

申请日：2020-04-24

Applicant: ALLOGENE THERAPEUTICS, INC.

Inventor： Diego A. VARGAS-INCHAUSTEGUI ,  Thomas Charles PERTEL ,  Barbra Johnson SASU

IPC: C12N5/00 ,  C12N5/0783 ,  A61K35/17

Abstract: Described herein are improved media for culturing immune cells, and methods of use thereof. In particular, cell growth media described herein are particularly suitable for T-cell expansion, which can be used for manufacture of cells useful in adoptive cell therapies, including therapies using chimeric antigen receptors (e.g., CAR-T cell therapy).

公开(公告)号：US20240360196A1

公开(公告)日：2024-10-31

申请号：US18607387

申请日：2024-03-15

Applicant: ALLOGENE THERAPEUTICS, INC. ,  CELLECTIS

Inventor： Arvind RAJPAL ,  Shobha Chowdary POTLURI ,  Laurent POIROT ,  Alexandre JUILLERAT ,  Thomas Charles PERTEL ,  Donna Marie STONE ,  Barbra Johnson SASU

IPC: C07K14/705 ,  A61K35/17 ,  A61K39/00 ,  C07K14/725 ,  C07K16/28 ,  C07K16/30 ,  C12N5/0783

CPC classification number: C07K14/70503 ,  A61K35/17 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C07K16/2803 ,  C07K16/3069 ,  C12N5/0636 ,  A61K2039/5156 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/03 ,  C07K2319/70 ,  C07K2319/74 ,  C12N2510/00

Abstract: The invention relates to an inhibitory chimeric antigen receptor (N-CAR) comprising



an extracellular domain comprising an antigen binding domain,
a transmembrane domain and,
an intracellular domain
wherein the intracellular domain comprises an Immunoreceptor Tyrosine-based Switch Motif ITSM, wherein said ITSM is a sequence of amino acid TX1YX2X3X4, wherein
X1 is an amino acid
X2 is an amino acid
X3 is an amino acid and
X4 is V or I.

公开(公告)号：US20240085403A1

公开(公告)日：2024-03-14

申请号：US18450554

申请日：2023-08-16

Applicant: Allogene Therapeutics, Inc.

Inventor： Thomas Charles PERTEL ,  Ren SONG ,  Diego A. VARGAS-INCHAUSTEGUI ,  Garima YAGNIK ,  Houman DEHGHANI ,  Wenjing LI ,  Jose PENA

IPC: G01N33/50 ,  C12N5/0783 ,  C12N7/00 ,  C12Q1/6851 ,  C12Q1/70

CPC classification number: G01N33/5023 ,  C12N5/0636 ,  C12N7/00 ,  C12Q1/6851 ,  C12Q1/705 ,  C12N2501/24 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2710/16521

Abstract: The instant disclosure relates to methods for preventing, controlling and/or inhibiting the rare events of adventitious viral infection or viral replication/amplification after reactivation of latent viral infection during cell culture in the manufacturing process of cell-based drug products, including CAR T cell drug products. Also provided are in vitro cell culture models for HHV-6 latent infection and methods of making the same.

公开(公告)号：US20240277763A1

公开(公告)日：2024-08-22

申请号：US18395996

申请日：2023-12-26

Applicant: ALLOGENE THERAPEUTICS, INC.

Inventor： Thomas Charles PERTEL ,  Barbra Johnson SASU ,  Mark W. LEONARD

IPC: A61K35/17 ,  A61K39/00 ,  A61P35/00 ,  C07K14/705 ,  C07K16/28 ,  C12N5/0783

CPC classification number: A61K35/17 ,  A61P35/00 ,  C07K14/70517 ,  C07K14/70596 ,  C07K16/2803 ,  C12N5/0636 ,  A61K2039/505 ,  C07K2317/76 ,  C07K2319/33 ,  C12N2510/00 ,  C12N2740/15043

Abstract: Provided herein are polynucleotides encoding chimeric antigen receptors (CARs) comprising a CD19 antigen binding domain that specifically birnds to CD19 and is resistant to rituximab binding; and immune cells comprising these CD19-specific CARs, e.g., CAR-T cells. Also provided are methods of making and using these CD19-specific CARs, and immune cells comprising these CD19-specific CARs.

公开(公告)号：US20230407252A1

公开(公告)日：2023-12-21

申请号：US18308797

申请日：2023-04-28

Applicant: Allogene Therapeutics, Inc.

Inventor： Meng-Yin LIN ,  Hayung YOON ,  Thomas Charles PERTEL ,  Barbra Johnson SASU

IPC: C12N5/0783 ,  C07K14/725

CPC classification number: C12N5/0636 ,  C07K14/7051 ,  C12N2510/00 ,  C07K2319/03 ,  C07K2319/02

Abstract: Provided herein are methods, kits and reagents for analyzing the attributes of cell populations, such as donor cells prior to modification to provide engineered cells, e.g., engineered immune cells, such as CAR T cells. For example, provided herein are methods of determining the amount or percentage of biomarkers and/or secretion profiles of donor cell populations, selecting donor cells with certain biomarkers and/or secretion profiles, and engineering the CAR T cells from the selected donor cells.

公开(公告)号：US20210062150A1

公开(公告)日：2021-03-04

申请号：US17010707

申请日：2020-09-02

Applicant: Allogene Therapeutics, Inc.

Inventor： Yajin NI ,  Chupei ZHANG ,  Mark W. LEONARD ,  Thomas Charles PERTEL

IPC: C12N5/0783

Abstract: Provided herein are methods for preparing T cells for T cell therapy comprising contacting a cell population at a predetermined cell density, with a concentration of an anti-CD3/CD28 nanomatrix and culturing the cells thereby producing a T cell population comprising an increased percentage of at least one T cell subtype. In some embodiments, the method increases the percentage of stem memory T cells.

公开(公告)号：US20200331998A1

公开(公告)日：2020-10-22

申请号：US16851691

申请日：2020-04-17

Applicant: ALLOGENE THERAPEUTICS, INC.

Inventor： Thomas Charles PERTEL ,  Barbra Johnson SASU ,  Tao SAI

IPC: C07K16/28 ,  A61K35/17

Abstract: Provided herein are isolated antibodies that specifically bind anti-CD19 chimeric antigen receptors (CARs) derived from a 4G7 scFv. Also provided are methods of making and using these isolated antibodies.