公开(公告)号：US20190216037A1

公开(公告)日：2019-07-18

申请号：US16246308

申请日：2019-01-11

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew Carl ADAMS ,  Dongmei XU

IPC: A01H6/82 ,  A01H5/12 ,  A01H1/06 ,  A24B3/12 ,  A24B3/08

CPC classification number: A01H6/823 ,  A01H1/06 ,  A01H5/12 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/20 ,  A24B15/243 ,  C12N15/8218

Abstract: The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20200071717A1

公开(公告)日：2020-03-05

申请号：US16674838

申请日：2019-11-05

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Alec J. HAYES ,  Marcos Fernando de GODOY LUSSO ,  Jerry Whit MORRIS

IPC: C12N15/82 ,  A24D3/04 ,  A24D1/00 ,  A24B13/00 ,  A24F47/00

Abstract: This disclosure provides tobacco plants containing a PMT RNAi and tobacco plants having a mutation in PMT, and methods of making and using such plants.

公开(公告)号：US20240292804A1

公开(公告)日：2024-09-05

申请号：US18424173

申请日：2024-01-26

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew Carl ADAMS ,  Dongmei XU

IPC: A01H6/82 ,  A01H1/06 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/10 ,  A24B15/20 ,  A24B15/24 ,  C12N15/82

CPC classification number: A01H6/823 ,  A01H1/06 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/10 ,  A24B15/20 ,  A24B15/243 ,  C12N15/8218 ,  C12N15/8227

Abstract: The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20160374387A1

公开(公告)日：2016-12-29

申请号：US15192480

申请日：2016-06-24

Applicant: Altria Client Services LLC

Inventor： Andrew Carl ADAMS ,  Marcos Fernando de GODOY LUSSO ,  Sreepriya PRAMOD ,  Dongmei XU

IPC: A24B15/10 ,  A24D1/04 ,  A24D3/04 ,  A24B13/02 ,  A01H5/12 ,  A24B3/12

CPC classification number: A24B15/10 ,  A01H5/12 ,  A24B3/12 ,  A24B13/00 ,  A24B13/02 ,  A24D1/045 ,  A24D3/043 ,  C12N15/8243

Abstract: The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.

Abstract translation: 本公开提供烟草Nic1基因座的鉴定。 还提供了具有改变的总生物碱和尼古丁水平和商业上可接受的叶子品级，通过育种或转基因方法发展的烟草植物以及来自这些烟草植物的烟草制品的生产。 还提供了用于生产具有新型Nic1突变或等位基因以减少尼古丁水平的烟草植物的组合物和方法。 进一步提供的是序列多态性和分子标记，用于在保持烟叶等级和烟草产品质量的同时养殖烟草，减少尼古丁或尼古丁。

公开(公告)号：US20240365741A1

公开(公告)日：2024-11-07

申请号：US18780938

申请日：2024-07-23

Applicant: Altria Client Services LLC

Inventor： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew Carl ADAMS ,  Dongmei XU

IPC: A01H6/82 ,  A01H1/06 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/10 ,  A24B15/20 ,  A24B15/24 ,  C12N15/82

Abstract: The present disclosure provides tobacco Nic1b locus and associated genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20240117370A1

公开(公告)日：2024-04-11

申请号：US18517109

申请日：2023-11-22

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Marcos Fernando de GODOY LUSSO ,  James A. STRICKLAND ,  Jesse FREDERICK ,  Dongmei XU ,  Chengalrayan KUDITHIPUDI

IPC: C12N15/82 ,  A24B13/00

CPC classification number: C12N15/8225 ,  A24B13/00 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8261

Abstract: The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US20220010324A1

公开(公告)日：2022-01-13

申请号：US17337161

申请日：2021-06-02

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew ADAMS ,  Dongmei XU

IPC: C12N15/82

Abstract: The present disclosure provides tobacco having genetic modification(s) in a group of ERF genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20220204981A1

公开(公告)日：2022-06-30

申请号：US17571138

申请日：2022-01-07

Applicant: Altria Client Services LLC

Inventor： Marcos Fernando de GODOY LUSSO ,  James A. STRICKLAND ,  Jesse FREDERICK ,  Dongmei XU ,  Chengalrayan KUDITHIPUDI

IPC: C12N15/82 ,  A24B13/00

Abstract: The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US20190271000A1

公开(公告)日：2019-09-05

申请号：US16291878

申请日：2019-03-04

Applicant: Altria Client Services LLC

Inventor： Marcos Fernando de GODOY LUSSO ,  James A STRICKLAND ,  Jesse FREDERICK ,  Dongmei XU ,  Chengalrayan KUDITHIPUDI

IPC: C12N15/82

Abstract: The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US20190246596A1

公开(公告)日：2019-08-15

申请号：US16246281

申请日：2019-01-11

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew Carl ADAMS ,  Dongmei XU

IPC: A01H6/82 ,  A01H5/12 ,  C12N15/82 ,  A24B15/10

CPC classification number: A01H6/823 ,  A01H1/06 ,  A01H5/12 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/10 ,  A24B15/20 ,  A24B15/243 ,  C12N15/8218 ,  C12N15/8227

Abstract: The present disclosure provides tobacco Nic1b locus and associated genes. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.