公开(公告)号：US20240180189A1

公开(公告)日：2024-06-06

申请号：US18404227

申请日：2024-01-04

申请人： R.J. Reynolds Tobacco Company

发明人： Anthony Richard Gerardi ,  Crystal Dawn Hege Byrd ,  Thaddeus Jude Jackson ,  Chelsea Allison Cooke ,  John-Paul Mua ,  Kyle Ford

IPC分类号： A23J1/00 ,  A23J3/14 ,  A23K20/147 ,  A23L2/66 ,  A23L5/20 ,  A23L33/185 ,  A24B15/18 ,  A24B15/20 ,  A24B15/24 ,  C07K14/415 ,  C12N9/88

CPC分类号： A23J1/007 ,  A23J3/14 ,  A23K20/147 ,  A23L2/66 ,  A23L5/23 ,  A23L5/273 ,  A23L33/185 ,  A24B15/18 ,  A24B15/20 ,  A24B15/24 ,  A24B15/241 ,  C07K14/415 ,  C12N9/88 ,  C12Y401/01039 ,  A23V2002/00

摘要： The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and/or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and/or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg/g; and a heavy metal content of less than about 60 μg/g.

公开(公告)号：US11999960B2

公开(公告)日：2024-06-04

申请号：US16484042

申请日：2018-02-06

申请人： University of Kentucky Research Foundation

发明人： George Wagner ,  Antoaneta Borissova Mahaylova-Kroumova ,  Guiliang Tang ,  Erming Wang

IPC分类号： C12N15/82 ,  A24B3/12 ,  A24B13/00 ,  A24B15/18 ,  A24B15/20

CPC分类号： C12N15/8218 ,  A24B3/12 ,  A24B13/00 ,  A24B15/183 ,  A24B15/20 ,  C12N15/8245

摘要： The present invention provides a method for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, the method comprising modifying said tobacco plant or tobacco cell culture by inhibiting the activity or expression of a diterpene synthesis gene. The present invention also provides for the use of a diterpene synthesis gene for increasing the sucrose ester content of a tobacco plant or tobacco cell culture, as well as tobacco cells, tobacco plants, tobacco plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

公开(公告)号：US20240041093A1

公开(公告)日：2024-02-08

申请号：US18483928

申请日：2023-10-10

申请人： Japan Tobacco Inc.

发明人： Ryosuke NAGASE

IPC分类号： A24B15/20 ,  A24B15/24

CPC分类号： A24B15/20 ,  A24B15/241

摘要： Provided is a tobacco-flavored liquid manufacturing method that includes processing a tobacco liquid extract using a material containing an inorganic porous body, and removing microorganisms from the tobacco liquid extract.

公开(公告)号：US11712415B2

公开(公告)日：2023-08-01

申请号：US17173891

申请日：2021-02-11

申请人： Philip Morris USA Inc.

发明人： Feng Gao ,  Shalva Gedevanishvili ,  Shengsheng Liu ,  Munmaya K. Mishra ,  William R. Sweeney ,  Randall Baren ,  Qinglin Li ,  Darin Colassaco ,  Salem Chouchane

IPC分类号： A61K9/00 ,  A61K36/82 ,  A23G4/08 ,  A23G4/12 ,  A61K36/81 ,  A23G4/06 ,  A24B13/00 ,  A24B15/18 ,  A24B15/20 ,  A61K47/36

CPC分类号： A61K9/0056 ,  A23G4/068 ,  A23G4/08 ,  A23G4/12 ,  A24B13/00 ,  A24B15/18 ,  A24B15/20 ,  A61K36/81 ,  A61K36/82 ,  A61K47/36 ,  A23V2002/00 ,  A23V2002/00 ,  A23V2200/26 ,  A23V2250/20

摘要： A soft, chewable and orally dissolvable and/or disintegrable product includes a biopolymer-sugar based matrix and botanical powder dispersed throughout the biopolymer-sugar based matrix. The biopolymer-sugar based matrix includes at least one biopolymer, at least one sugar and optional additives. Soft, chewable and orally dissolvable and/or disintegrable product can also include flavor beads.

公开(公告)号：US20190216037A1

公开(公告)日：2019-07-18

申请号：US16246308

申请日：2019-01-11

申请人： ALTRIA CLIENT SERVICES LLC

发明人： Sreepriya PRAMOD ,  Marcos Fernando de GODOY LUSSO ,  Jesse FREDERICK ,  Andrew Carl ADAMS ,  Dongmei XU

IPC分类号： A01H6/82 ,  A01H5/12 ,  A01H1/06 ,  A24B3/12 ,  A24B3/08

CPC分类号： A01H6/823 ,  A01H1/06 ,  A01H5/12 ,  A24B3/08 ,  A24B3/12 ,  A24B13/00 ,  A24B15/20 ,  A24B15/243 ,  C12N15/8218

摘要： The present disclosure provides tobacco Nic1b locus and associated genes (e.g., a group of ERF genes). Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Further provided are compositions and methods for producing tobacco plants having novel mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or alkaloids while maintaining tobacco leaf grade and tobacco product quality.

公开(公告)号：US20190194733A1

公开(公告)日：2019-06-27

申请号：US16299881

申请日：2019-03-12

申请人： U.S. Smokeless Tobacco Company LLC

发明人： Dongmei Xu ,  Mark T. Nielsen ,  Yanxin Shen

IPC分类号： C12Q1/6844 ,  C12N9/02 ,  C12N15/82 ,  C07K14/415 ,  A24B15/10

CPC分类号： A24B15/20 ,  A24B15/10 ,  C07K14/415 ,  C12N9/0077 ,  C12N15/8261 ,  C12Q1/6844 ,  C12Q2560/00 ,  C12Q2600/13

摘要： The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.

公开(公告)号：US20180177224A1

公开(公告)日：2018-06-28

申请号：US15902397

申请日：2018-02-22

申请人： R.J. Reynolds Tobacco Company

发明人： Jerry Wayne Marshall ,  Anthony Richard Gerardi ,  Luis Monsalud, JR. ,  Alton Busbee ,  Jo Ann Hill Hart ,  Annett Milling

IPC分类号： A24B15/28 ,  A24B15/42 ,  A24B15/30 ,  A24B13/00 ,  A24B15/24 ,  A24B15/20

CPC分类号： A24B15/28 ,  A24B13/00 ,  A24B15/20 ,  A24B15/245 ,  A24B15/287 ,  A24B15/30 ,  A24B15/307 ,  A24B15/42

摘要： Methods of modifying the tobacco-specific nitrosamine content of a tobacco material are described herein. One exemplary method comprises contacting a tobacco material with a composition comprising salt, sugar, enzyme, lactic acid bacteria, yeast, or a combination thereof to reduce the total bacterial content; curing the tobacco material; and fermenting the tobacco material in the presence of one or more microorganisms. The method can provide a fermented tobacco material having a tobacco-specific nitrosamine content that is reduced relative to a fermented tobacco material that has not been subjected to the disclosed method steps. In certain embodiments, the tobacco-specific nitrosamine content of the fermented tobacco material is no more than that of the cured tobacco material. Tobacco-containing products including such treated tobacco materials are also provided.

公开(公告)号：US09781929B2

公开(公告)日：2017-10-10

申请号：US15009926

申请日：2016-01-29

申请人： Altria Client Services LLC ,  Micreos BV

发明人： Elisabeth Miller ,  Ujwala Warek ,  Dongmei Xu ,  Fritz Eichenseher ,  Steven Hagens ,  Chengalrayan Kudithipudi

IPC分类号： A24B15/20 ,  C07K14/005 ,  C12N7/00 ,  A01N37/18 ,  A24B15/18 ,  A24B15/24 ,  A24B15/30

CPC分类号： A01N37/18 ,  A24B15/183 ,  A24B15/20 ,  A24B15/245 ,  A24B15/30 ,  C07K14/005 ,  C12N7/00 ,  C12N2795/00021 ,  C12N2795/00022 ,  C12N2795/00031

摘要： Bacteriophage are provided, and methods of making and using the bacteriophage also are provided.

公开(公告)号：US09681681B2

公开(公告)日：2017-06-20

申请号：US14870526

申请日：2015-09-30

申请人： R.J. REYNOLDS TOBACCO COMPANY

发明人： Serban C. Moldoveanu ,  Marvin Glenn Riddick ,  Jerry Wayne Marshall

IPC分类号： A24B15/20 ,  A24B15/30 ,  A24B1/00 ,  A24B15/18

CPC分类号： A24B15/20 ,  A24B1/00 ,  A24B15/183 ,  A24B15/307

摘要： A method of modifying the content of certain bacteria in uncured tobacco material is provided, the method including contacting an uncured tobacco material with a treatment solution, wherein the treatment solution is selected from the group consisting of: (i) a solution comprising salt, sugar, or a combination thereof; (ii) a solution comprising one or more enzymes; and (iii) a solution comprising one or more probiotics, wherein said contacting provides a treated tobacco material having a reduced total bacterial content following harvest. In certain embodiments, the treated tobacco material is subsequently cured, and can optionally be fermented. Smoking articles and smokeless tobacco products including such treated tobacco materials are also provided.

公开(公告)号：US20170166913A1

公开(公告)日：2017-06-15

申请号：US15422460

申请日：2017-02-01

申请人： 22nd Century Limited, LLC

发明人： Takashi HASHIMOTO ,  Akira KATO

IPC分类号： C12N15/82 ,  A24F47/00 ,  A24B13/00 ,  A24D1/00 ,  C12Q1/68 ,  C07K14/415

CPC分类号： A24B15/18 ,  A01H5/12 ,  A23V2002/00 ,  A24B13/00 ,  A24B13/02 ,  A24B15/20 ,  A24B15/243 ,  A24D1/00 ,  C07K14/415 ,  C12N15/8218 ,  C12N15/8243 ,  C12Q2600/158

摘要： Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.