公开(公告)号：US20240132901A1

公开(公告)日：2024-04-25

申请号：US18365270

申请日：2023-08-03

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Rajanikanth GOVINDARAJULU ,  Raja S. PAYYAVULA

IPC: C12N15/82 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

CPC classification number: C12N15/8243 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

Abstract: The present disclosure relates to tobacco plants, plant parts, seeds, compositions, and methods related to modulating the expression of novel genes in tobacco to regulate alkaloid levels.

公开(公告)号：US20240117370A1

公开(公告)日：2024-04-11

申请号：US18517109

申请日：2023-11-22

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Marcos Fernando de GODOY LUSSO ,  James A. STRICKLAND ,  Jesse FREDERICK ,  Dongmei XU ,  Chengalrayan KUDITHIPUDI

IPC: C12N15/82 ,  A24B13/00

CPC classification number: C12N15/8225 ,  A24B13/00 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8261

Abstract: The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US20240018534A1

公开(公告)日：2024-01-18

申请号：US18450522

申请日：2023-08-16

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Jaemo YANG ,  Yanxin SHEN ,  Dongmei XU ,  Ujwala WAREK ,  James Strickland ,  Jesse FREDERICK

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8229

Abstract: The present disclosure provides the identification of promoters that are preferentially active in tobacco axillary buds. Also provided are tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US20230122459A1

公开(公告)日：2023-04-20

申请号：US17936001

申请日：2022-09-28

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Alec J. HAYES

IPC: C12N15/82 ,  A01H1/06 ,  A24B13/00

Abstract: This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

公开(公告)号：US20230024503A1

公开(公告)日：2023-01-26

申请号：US17954654

申请日：2022-09-28

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Rutger VAN DER HOEVEN ,  Ndjido Ardo KANE

IPC: A01H6/82 ,  A01H5/12 ,  A24D1/00 ,  A24B13/00 ,  A01H1/06

Abstract: A process for producing a reduced tobacco-specific nitrosamine (TSNA) tobacco plant comprising reducing and/or eliminating anatabine biosynthesis in wild-type tobacco plant. In addition, use of such plants for discovery of molecular markers that are closely linked with genes required for anatabine biosynthesis and for discovery of genes required for anatabine biosynthesis. In addition, a smoking composition, a smoking article and a smokeless tobacco oral delivery product contain the tobacco material.

公开(公告)号：US20240102038A1

公开(公告)日：2024-03-28

申请号：US18537046

申请日：2023-12-12

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Dongmei XU ,  Jesse FREDERICK ,  Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  James STRICKLAND ,  Jaemo YANG

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8265 ,  C07K14/415 ,  C12N15/8213 ,  C12N15/8218 ,  C12N15/8225 ,  C12N15/8229 ,  C12N15/8261 ,  C12N15/8262 ,  C12N15/8294 ,  C12N2310/20

Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.

公开(公告)号：US20240018536A1

公开(公告)日：2024-01-18

申请号：US18449849

申请日：2023-08-15

Applicant: ALTRIA CLIENT SERVICES LLC ,  UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  Dongmei XU ,  Michael Paul TIMKO ,  Roel RABARA

IPC: C12N15/82 ,  C07K14/415 ,  C12P5/00

CPC classification number: C12N15/8262 ,  C12N15/8243 ,  C07K14/415 ,  C12N15/8223 ,  C12P5/007 ,  C12N2800/22

Abstract: The present disclosure relates to compositions and methods related to tissue-specific promoters and their uses in plants, including tobacco and Cannabis. The provided trichome-specific promoters enable the expression of heterologous polynucleotides in trichome tissues.

公开(公告)号：US20230348925A1

公开(公告)日：2023-11-02

申请号：US18182701

申请日：2023-03-13

Applicant: Altria Client Services LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Dongmei XU ,  James Strickland

IPC: C12N15/82 ,  C12N9/04 ,  A24B15/24 ,  B01L3/00 ,  G01N1/38 ,  H02N11/00 ,  B01F33/45 ,  B01F33/452 ,  B01F33/501 ,  B01F35/75 ,  A01H4/00 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415

CPC classification number: C12N15/8243 ,  C12N9/0006 ,  A24B15/245 ,  C12N15/825 ,  B01L3/502 ,  B01L3/50825 ,  G01N1/38 ,  H02N11/006 ,  B01F33/45 ,  B01F33/452 ,  B01F33/50112 ,  B01F35/754251 ,  A01H4/005 ,  A01H4/008 ,  A24B13/00 ,  A24B15/10 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8225 ,  B01L2200/0684 ,  B01L2300/043 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0403 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0683 ,  G01N35/1079

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20220243216A1

公开(公告)日：2022-08-04

申请号：US17591057

申请日：2022-02-02

Applicant: ALTRIA CLIENT SERVICES LLC ,  UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  Dongmei XU ,  Michael Paul TIMKO ,  Roel RABARA

IPC: C12N15/82

Abstract: The present disclosure relates to compositions and methods related to modification of trichome density and transport of metabolite and their uses in plants, including tobacco and cannabis. The provided transcription factors enable the increase in trichome density in plants.

公开(公告)号：US20220098604A1

公开(公告)日：2022-03-31

申请号：US17551565

申请日：2021-12-15

Applicant: ALTRIA CLIENT SERVICES LLC

Inventor： Chengalrayan KUDITHIPUDI ,  Yanxin SHEN ,  Dongmei XU ,  Jesse FREDERICK ,  Jaemo YANG

IPC: C12N15/82 ,  C07K14/415

Abstract: This disclosure provides a number of sequences involved in axillary bud growth in tobacco, methods of using such sequences, tobacco plants carrying modifications to such sequences or transgenes of such sequences, and tobacco products made from tobacco leaf harvested from such plants.