公开(公告)号：US10513723B2

公开(公告)日：2019-12-24

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US11254963B2

公开(公告)日：2022-02-22

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C07K16/00 ,  C12N1/38 ,  C07K14/52 ,  C12N5/071

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.