公开(公告)号：US10227627B2

公开(公告)日：2019-03-12

申请号：US16130879

申请日：2018-09-13

Applicant: AMGEN INC.

Inventor： Shivani Gupta ,  Sohye Kang

IPC: A61K38/14 ,  C12Q1/48 ,  C12P21/00 ,  C07K16/00 ,  C07K14/705 ,  C12N9/10

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US10513723B2

公开(公告)日：2019-12-24

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US10106829B2

公开(公告)日：2018-10-23

申请号：US15115615

申请日：2014-12-11

Applicant: AMGEN INC.

Inventor： Shivani Gupta ,  Sohye Kang

IPC: C12P21/00 ,  C12N5/10 ,  A61K38/14 ,  C12N9/10 ,  C07K14/705 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US11254963B2

公开(公告)日：2022-02-22

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C07K16/00 ,  C12N1/38 ,  C07K14/52 ,  C12N5/071

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US10655156B2

公开(公告)日：2020-05-19

申请号：US16261311

申请日：2019-01-29

Applicant: AMGEN INC.

Inventor： Shivani Gupta ,  Sohye Kang

IPC: A61K38/14 ,  C12Q1/48 ,  C12P21/00 ,  C07K16/00 ,  C07K14/705 ,  C12N9/10 ,  C12N15/85

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.

公开(公告)号：US10907186B2

公开(公告)日：2021-02-02

申请号：US16875832

申请日：2020-05-15

Applicant: AMGEN INC.

Inventor： Shivani Gupta ,  Sohye Kang

IPC: C12N15/00 ,  A61K38/00 ,  C12P21/00 ,  C12N9/10 ,  C12N15/85 ,  C07K14/705 ,  C07K16/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the overexpression of proteins involved in the N-glycosylation pathway.