公开(公告)号：US20240026432A1

公开(公告)日：2024-01-25

申请号：US18029995

申请日：2021-10-15

Applicant: AMGEN INC.

Inventor： Neil Soice ,  Scott Kuhns ,  Andrew Csordas

IPC: C12Q1/6848 ,  C12Q1/6806

CPC classification number: C12Q1/6848 ,  C12Q1/6806

Abstract: The disclosure provides materials and methods for assessing the presence and level of polyanionic compounds in a sample that inhibit PCR reactions, including assays for the presence, and optionally the amount, of polyvinyl sulfonate, as well as materials and methods for the reduction or removal of such compounds from buffer solutions and from protein solutions using a variety of approaches, including titration-based techniques.

公开(公告)号：US20240051990A1

公开(公告)日：2024-02-15

申请号：US18268772

申请日：2022-03-10

Applicant: AMGEN INC.

Inventor： Neil Soice ,  Jeremy S. Conner ,  Glenn M. Hunter ,  Kenneth Shoemaker ,  Bret Wylie ,  John B. Flynn ,  Nakorn Kaeonil ,  Thuy N. Nguyen ,  Siddharth Singh

IPC: C07K1/36

CPC classification number: C07K1/36

Abstract: Parallel chromatography systems and continuous manufacturing methods are described herein that utilize two or more chromatography column skids having columns operating in parallel with automation controls governing which column to load at a given time.

公开(公告)号：US20240075406A1

公开(公告)日：2024-03-07

申请号：US18268683

申请日：2022-02-17

Applicant: AMGEN INC.

Inventor： Jeremy S. Conner ,  Glenn M. Hunter ,  Kenneth Shoemaker ,  Neil Soice ,  Bret Wylie ,  John B. Flynn ,  Nakorn Kaeonil ,  Thuy N. Nguyen ,  Siddharth Sigh

IPC: B01D15/18 ,  C07K1/16

CPC classification number: B01D15/1885 ,  C07K1/16

Abstract: Parallel chromatography systems and continuous manufacturing methods are described herein that utilize two or more chromatography column skids having columns operating in parallel with automation controls governing which column to load at a given time.

公开(公告)号：US20210395298A1

公开(公告)日：2021-12-23

申请号：US17283135

申请日：2019-10-11

Applicant: AMGEN INC.

Inventor： Pavel Bondarenko ,  Pik Chan ,  Neil Soice

IPC: C07K1/18 ,  C07K16/28

Abstract: The present invention provides an improved manufacturing method for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the method comprising a step of refolding the construct, wherein one or more of the domains comprise a disulfide bond, and wherein the construct is contacted with a refolding buffer to refold the construct to its native form, the buffer comprising (i) a redox and/or oxidizing agent having a concentration of at least 0.1 mM and (ii) a chaotropic agent having a concentration >1 M, and wherein the pH of the refolding buffer corresponds to +/−4.5 of the pI value of the first domain or +/−4.0 of the pI value of the entire construct.