公开(公告)号：US20150238919A1

公开(公告)日：2015-08-27

申请号：US14610814

申请日：2015-01-30

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Mark F. OLDHAM ,  Dar BAHATT ,  Kenneth J. LIVAK ,  Jason E. BABCOKE ,  H. Pin KAO ,  Stephen J. GUNSTREAM ,  Kevin S. BODNER ,  Douglas P. GREINER ,  Nigel P. BEARD

IPC: B01J19/00 ,  G01N21/17 ,  B01L7/00

CPC classification number: B01J19/0046 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/185 ,  B01L2400/0655 ,  G01N21/17 ,  G01N2201/0407

Abstract: Exemplary embodiments provide microfluidic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

Abstract translation: 示例性实施例提供了用于其使用的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。

公开(公告)号：US20140199715A1

公开(公告)日：2014-07-17

申请号：US14159353

申请日：2014-01-20

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Dar BAHATT ,  Konrad FAULSTICH

IPC: C12Q1/66

CPC classification number: C12Q1/66 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/66 ,  G01N21/763 ,  G01N33/581 ,  G01N2021/6439 ,  G01N2021/6469

Abstract: The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source.

公开(公告)号：US20140080159A1

公开(公告)日：2014-03-20

申请号：US13968128

申请日：2013-08-15

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Dar BAHATT ,  Konrad FAULSTICH

IPC: G01N21/76

CPC classification number: C12Q1/66 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/66 ,  G01N21/763 ,  G01N33/581 ,  G01N2021/6439 ,  G01N2021/6469

Abstract: The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source.

Abstract translation: 本教导提供了用于生物材料的检测单元和用于检测生物材料的方法，所述生物材料包括与包含生物材料的内部空间光学耦合的感光材料，以避免光学部件或外部光源。