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公开(公告)号：US20150238919A1
公开(公告)日：2015-08-27
申请号：US14610814
申请日：2015-01-30
Applicant: APPLIED BIOSYSTEMS, LLC
Inventor： Mark F. OLDHAM , Dar BAHATT , Kenneth J. LIVAK , Jason E. BABCOKE , H. Pin KAO , Stephen J. GUNSTREAM , Kevin S. BODNER , Douglas P. GREINER , Nigel P. BEARD
CPC classification number: B01J19/0046 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/027 , B01L2200/10 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0867 , B01L2300/185 , B01L2400/0655 , G01N21/17 , G01N2201/0407
Abstract: Exemplary embodiments provide microfluidic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.
Abstract translation: 示例性实施例提供了用于其使用的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。
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公开(公告)号：US20180016624A1
公开(公告)日：2018-01-18
申请号：US15658016
申请日：2017-07-24
Applicant: Applied Biosystems, LLC
Inventor： Min YUE , David M. LIU , Joy ROY , Yuh-Min CHIANG , Joon Mo YANG , Dennis LEHTO , Charles S. VANN , Nigel P. BEARD , Ian A. HARDING , John R. Van Camp , Alexander DROMARETSKY , Sergey V. ERMAKOV , Mark F. OLDHAM , Maryam SHARIATI , Umberto ULMANELLA
CPC classification number: C12Q1/6806 , B01J2219/00317 , B01J2219/00722 , B01L3/502707 , B01L3/502723 , B01L3/50853 , B01L2200/0684 , B01L2200/0689 , B01L2200/16 , B01L2300/041 , B01L2300/048 , B01L2300/0636 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , B01L2300/087 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0694 , G01N35/028 , Y10T436/2575
Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
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公开(公告)号：US20150338373A1
公开(公告)日：2015-11-26
申请号：US14817573
申请日：2015-08-04
Applicant: Applied Biosystems, LLC
Inventor： Charles S. VANN , Debjyoti BANERJEE , Timothy G. GEISER , James C. NURSE , Nigel P. BEARD
IPC: G01N27/447 , B01L3/00
CPC classification number: G01N27/44743 , B01J4/008 , B01J2219/00315 , B01J2219/0036 , B01J2219/00418 , B01J2219/00653 , B01L3/0241 , B01L3/0268 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/02 , B01L2400/0409 , B01L2400/0427
Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
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公开(公告)号：US20180128778A1
公开(公告)日：2018-05-10
申请号：US15614601
申请日：2017-06-05
Applicant: APPLIED BIOSYSTEMS, LLC
Inventor： Charles S. VANN , Debjyoti BANERJEE , Timothy G. GEISER , James C. NURSE , Nigel P. BEARD
IPC: G01N27/447 , B01L3/02 , B01J4/00 , B01L3/00
CPC classification number: G01N27/44743 , B01J4/008 , B01J2219/00315 , B01J2219/0036 , B01J2219/00418 , B01J2219/00653 , B01L3/0241 , B01L3/0268 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/02 , B01L2400/0409 , B01L2400/0427
Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
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