公开(公告)号：US20150238919A1

公开(公告)日：2015-08-27

申请号：US14610814

申请日：2015-01-30

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Mark F. OLDHAM ,  Dar BAHATT ,  Kenneth J. LIVAK ,  Jason E. BABCOKE ,  H. Pin KAO ,  Stephen J. GUNSTREAM ,  Kevin S. BODNER ,  Douglas P. GREINER ,  Nigel P. BEARD

IPC: B01J19/00 ,  G01N21/17 ,  B01L7/00

CPC classification number: B01J19/0046 ,  B01L3/502715 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/185 ,  B01L2400/0655 ,  G01N21/17 ,  G01N2201/0407

Abstract: Exemplary embodiments provide microfluidic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.

Abstract translation: 示例性实施例提供了用于其使用的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。

公开(公告)号：US20140238976A1

公开(公告)日：2014-08-28

申请号：US14171345

申请日：2014-02-03

Applicant: APPLIED BIOSYSTEMS, LLC

Inventor： Kirk HIRANO ,  Patrick KINNEY ,  Douwe HAGA ,  Jason BABCOKE ,  Albert CARRILLO ,  H. Pin KAO ,  James NURSE

IPC: B01L7/00 ,  H05B6/02 ,  H05B3/84

CPC classification number: B01L7/52 ,  B01L3/50851 ,  B01L3/50853 ,  B01L3/50857 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1816 ,  B01L2300/1838 ,  B01L2300/1861 ,  B01L2300/1872 ,  F28F13/00 ,  F28F2013/001 ,  G01N21/0332 ,  G01N21/6452 ,  G01N2201/062 ,  H05B3/84 ,  H05B6/02 ,  H05B6/105

Abstract: A heating apparatus comprising a support base and a microplate having a first surface and an opposing second surface. The microplate is positioned adjacent the support base and comprises a plurality of wells formed in the first surface thereof. Each of the plurality of wells is sized to receive an assay therein. A sapphire crystalline transparent window is positioned adjacent the microplate opposing the support base. A heating device heats the transparent window in response to a control system.