公开(公告)号：US11547997B2

公开(公告)日：2023-01-10

申请号：US16603338

申请日：2018-04-06

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander A. Green ,  Matthew Gilliam ,  Kirstie Swingle ,  Nicholas Stephanopoulos ,  Neal Woodbury

IPC: B01L3/00 ,  C12Q1/70 ,  G01N33/543

Abstract: Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

公开(公告)号：US11186835B2

公开(公告)日：2021-11-30

申请号：US16245984

申请日：2019-01-11

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander A. Green

IPC: C12N9/00 ,  C12P21/02 ,  C07K14/005 ,  C07K14/47 ,  C12N15/113 ,  C12P7/64

Abstract: Provided herein, in some embodiments, are artificial ribosomes that synthesize nonribosomal peptides, polyketides, and fatty acids with full control over peptide sequence. Also provided herein are methods for programmed synthesis of nonribosomal peptides, polyketides, and fatty acids. In particular, provided herein are methods for scalable synthesis of a wide range of antibacterial, antifungal, antiviral, and anticancer compounds.

公开(公告)号：US10550440B2

公开(公告)日：2020-02-04

申请号：US16079740

申请日：2017-02-27

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Alexander A. Green ,  Anli Tang

IPC: C12Q1/6897 ,  C12N15/113 ,  C12N15/67 ,  C07K14/435

Abstract: Provided herein is a RNA-based riboregulator that detects protein translation and uses this stimulus to regulate expression of an unrelated protein. In one embodiment, provided herein is a synthetic nucleic acid molecule configured as a translation-sensing riboregulator, where the synthetic nucleic acid molecule comprises a first nucleotide sequence encoding a polypeptide, a translation-sensing riboswitch (TSR), and a second nucleotide sequence encoding a polypeptide, wherein the translation-sensing riboswitch comprises a stop codon of the first nucleotide sequence, a fully or partially double-stranded stem-forming domain, and translation initiation elements of the second nucleotide sequence.