公开(公告)号：US20220243246A1

公开(公告)日：2022-08-04

申请号：US17590457

申请日：2022-02-01

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG

IPC: C12Q1/06 ,  C12Q1/18 ,  G06T7/00 ,  G06T7/246

Abstract: Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.

公开(公告)号：US20240344106A1

公开(公告)日：2024-10-17

申请号：US18751532

申请日：2024-06-24

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG

IPC: C12Q1/06 ,  C12Q1/18 ,  G06T7/00 ,  G06T7/246

CPC classification number: C12Q1/06 ,  C12Q1/18 ,  G06T7/0016 ,  G06T7/246 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06T2207/30241

Abstract: Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.

公开(公告)号：US20230278032A1

公开(公告)日：2023-09-07

申请号：US18174541

申请日：2023-02-24

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG

IPC: B01L3/00 ,  G06V10/14 ,  G06V10/25 ,  G06V10/44 ,  G06V10/62

CPC classification number: B01L3/502715 ,  B01L3/502761 ,  G06V10/14 ,  G06V10/25 ,  G06V10/44 ,  G06V10/62 ,  B01L2400/0487 ,  G01N2021/0106

Abstract: Disclosed herein is a system and method for quantitative detection and analysis of molecular binding kinetics of a substance with surface membrane proteins of a biological object, such as a live cell, based on detecting and tracking membrane fluctuation amplitude changes cause by membrane displacement associated with the binding of the substance with the surface membrane proteins. The molecular binding kinetics can be detected with high precision in real time from an optical image of the biological object with a differential detection method.

公开(公告)号：US20250012697A1

公开(公告)日：2025-01-09

申请号：US18055033

申请日：2022-11-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG

IPC: G01N15/0205 ,  G01N15/01 ,  G01N15/0227 ,  G06V10/774 ,  G06V20/69 ,  G16H10/40

Abstract: Provided herein is a method of detecting microorganisms in liquid samples using image features, such as time profiles of object light scattering intensity and time profiles of object position. Related methods, devices, systems, and other aspects are also provided.

公开(公告)号：US20230230249A1

公开(公告)日：2023-07-20

申请号：US18002438

申请日：2021-06-23

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG ,  Nongjian TAO

IPC: G06T7/00 ,  C12Q1/06 ,  C12Q1/18 ,  C12M1/00

CPC classification number: G06T7/0016 ,  C12Q1/06 ,  C12Q1/18 ,  C12M41/06

Abstract: Detecting single bacterial cells in a sample includes collecting, from a sample provided to an imaging apparatus, a multiplicity of images of the sample over a length of time; assessing a trajectory of each bacterial cell in the sample; and assessing, based on the trajectory of each bacterial cell in the sample, a number of bacterial cell divisions that occur in the sample during the length of time.