公开(公告)号：US20230112565A1

公开(公告)日：2023-04-13

申请号：US17799230

申请日：2021-02-12

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Nongjian TAO ,  Shaopeng WANG ,  Pengfei ZHANG

IPC: G01N33/68 ,  B01L3/00 ,  G01N33/543 ,  G01N15/14

Abstract: Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.

公开(公告)号：US20230230249A1

公开(公告)日：2023-07-20

申请号：US18002438

申请日：2021-06-23

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Fenni ZHANG ,  Nongjian TAO

IPC: G06T7/00 ,  C12Q1/06 ,  C12Q1/18 ,  C12M1/00

CPC classification number: G06T7/0016 ,  C12Q1/06 ,  C12Q1/18 ,  C12M41/06

Abstract: Detecting single bacterial cells in a sample includes collecting, from a sample provided to an imaging apparatus, a multiplicity of images of the sample over a length of time; assessing a trajectory of each bacterial cell in the sample; and assessing, based on the trajectory of each bacterial cell in the sample, a number of bacterial cell divisions that occur in the sample during the length of time.