公开(公告)号：US20230408409A1

公开(公告)日：2023-12-21

申请号：US18204735

申请日：2023-06-01

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Pengfei ZHANG

IPC: G01N21/55 ,  G01N33/543 ,  G16B15/00

CPC classification number: G01N21/55 ,  G01N33/54373 ,  G16B15/00 ,  G01N2021/556

Abstract: Provided herein are methods of detecting exosomes, including unlabeled exosomes. In some embodiments, the methods include disposing a fluidic sample that comprises a plurality of exosomes in a chamber that is positioned at least partially within a fluidic device in which an inner surface of the chamber comprises a first set of exosome binding moieties that are capable of binding the exosomes. In some embodiments, the methods also include binding a portion of the plurality of exosomes to a portion of the first set of exosome binding moieties to produce surface-bound exosomes, introducing an incident light toward the inner surface of the chamber prior to, concurrent with, and/or after, producing the surface-bound exosomes, and detecting light scattered by the surface-bound exosomes to produce a set of exosome imaging data. Related fluidic devices, systems, and computer readable media are also provided.

公开(公告)号：US20240003905A1

公开(公告)日：2024-01-04

申请号：US18212460

申请日：2023-06-21

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Rui WANG ,  Pengfei ZHANG

IPC: G01N33/68 ,  G01N33/543

CPC classification number: G01N33/6872 ,  G01N33/54386 ,  G01N2333/705

Abstract: Provided herein are methods of modulating temperature in detection fields and producing analyte imaging data. In some embodiments, the methods include introducing an incident light toward a second surface of a substrate to induce a plasmonic wave proximal to a first surface of the substrate such that a temperature in a selected heating space within the detection field is substantially uniformly changed to a selected temperature. Additional methods as well as related systems and computer readable media are also provided.

公开(公告)号：US20230112565A1

公开(公告)日：2023-04-13

申请号：US17799230

申请日：2021-02-12

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Nongjian TAO ,  Shaopeng WANG ,  Pengfei ZHANG

IPC: G01N33/68 ,  B01L3/00 ,  G01N33/543 ,  G01N15/14

Abstract: Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.