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1.发明公开公开(公告)号：US20240102113A1
公开(公告)日：2024-03-28
申请号：US18255731
申请日：2021-12-02
Inventor： Karen ANDERSON , Mark KNAPPENBERGER , Siril AROCKIAM
IPC: C12Q1/70 , C12N9/22 , C12N15/11 , C12Q1/6818
CPC classification number: C12Q1/70 , C12N9/22 , C12N15/11 , C12Q1/6818 , C12Q1/6806
Abstract: The disclosure relates to methods of detecting a target nucleic acid in a sample using a deactivated RNA-guided nuclease and guide RNA complex. Some methods relate to isolating protein-nucleic acid complexes using silica-based chromatography with certain buffered solutions to better prevent complex dissociation. Some methods relate to isolating protein-nucleic acid complexes using a tagged ribonucleoprotein which binds to a target nucleic acid to form a detectable interconnected network comprising the target nucleic acid. Also provided herein are primer nucleic acids, buffer solutions, and kits for performing the methods of this disclosure for detecting a target nucleic acid in a sample.
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2.发明申请公开(公告)号：US20240401159A1
公开(公告)日：2024-12-05
申请号：US18694303
申请日：2022-09-22
Inventor： Karen ANDERSON , Mark KNAPPENBERGER , Siril AROCKIAM
IPC: C12Q1/70 , C12Q1/6848
Abstract: Provided herein are methods for multiplexed LAMP-based detection of multiple target nucleic acids in parallel at a single reaction temperature. In particular, provided herein are methods for performing a plurality of LAMP reactions in parallel using the addition of LAMP reaction temperature-shifting agents to better optimize a subset of the LAMP reactions to a single reaction temperature. For certain LAMP reactions, provided herein are LAMP primer sets and respective LAMP temperature-shifting parameters to provide efficient LAMP readouts at specific temperatures. Also provided herein are methods for increasing end-point signal-to-noise ratios of LAMP assays based on the detection of a fluorescent intercalating-dye in an amplification product.
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