公开(公告)号：US20210205435A1

公开(公告)日：2021-07-08

申请号：US16762085

申请日：2018-11-15

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI ,  BAYLOR COLLEGE OF MEDICINE

Inventor： Sri KRISHNA ,  Marshall POSNER ,  Andrew SIKORA ,  Karen ANDERSON

IPC: A61K39/12 ,  A61P31/20 ,  A61P35/00

Abstract: Embodiments of the present disclosure pertain generally to head and neck squamous cell carcinomas (HNSCCs) related to human papillomavirus subtype 16 (HPV16) infections. More particularly, the present disclosure provides novel immunogenic epitopes from HPV16 E2, E6 and E7 antigens restricted by common human leukocyte antigen (HLA) alleles for the diagnosis and treatment of HNSCC. The HPV16 epitopes identified in the present disclosure can be used in combination with blockade of HPV16+HNSCC-specific checkpoints for targeted immunotherapy.

公开(公告)号：US20250073325A1

公开(公告)日：2025-03-06

申请号：US18597598

申请日：2024-03-06

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI ,  BAYLOR COLLEGE OF MEDICINE

Inventor： Sri KRISHNA ,  Marshall POSNER ,  Andrew SIKORA ,  Karen ANDERSON

IPC: A61K39/12 ,  A61K39/00 ,  A61P31/20 ,  A61P35/00

Abstract: Embodiments of the present disclosure pertain generally to head and neck squamous cell carcinomas (HNSCCs) related to human papillomavirus subtype 16 (HPV16) infections. More particularly, the present disclosure provides novel immunogenic epitopes from HPV16 E2, E6 and E7 antigens restricted by common human leukocyte antigen (HLA) alleles for the diagnosis and treatment of HNSCC. The HPV16 epitopes identified in the present disclosure can be used in combination with blockade of HPV16+ HNSCC-specific checkpoints for targeted immunotherapy.

公开(公告)号：US20240102113A1

公开(公告)日：2024-03-28

申请号：US18255731

申请日：2021-12-02

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Karen ANDERSON ,  Mark KNAPPENBERGER ,  Siril AROCKIAM

IPC: C12Q1/70 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6818

CPC classification number: C12Q1/70 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6818 ,  C12Q1/6806

Abstract: The disclosure relates to methods of detecting a target nucleic acid in a sample using a deactivated RNA-guided nuclease and guide RNA complex. Some methods relate to isolating protein-nucleic acid complexes using silica-based chromatography with certain buffered solutions to better prevent complex dissociation. Some methods relate to isolating protein-nucleic acid complexes using a tagged ribonucleoprotein which binds to a target nucleic acid to form a detectable interconnected network comprising the target nucleic acid. Also provided herein are primer nucleic acids, buffer solutions, and kits for performing the methods of this disclosure for detecting a target nucleic acid in a sample.

公开(公告)号：US20240401159A1

公开(公告)日：2024-12-05

申请号：US18694303

申请日：2022-09-22

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Karen ANDERSON ,  Mark KNAPPENBERGER ,  Siril AROCKIAM

IPC: C12Q1/70 ,  C12Q1/6848

Abstract: Provided herein are methods for multiplexed LAMP-based detection of multiple target nucleic acids in parallel at a single reaction temperature. In particular, provided herein are methods for performing a plurality of LAMP reactions in parallel using the addition of LAMP reaction temperature-shifting agents to better optimize a subset of the LAMP reactions to a single reaction temperature. For certain LAMP reactions, provided herein are LAMP primer sets and respective LAMP temperature-shifting parameters to provide efficient LAMP readouts at specific temperatures. Also provided herein are methods for increasing end-point signal-to-noise ratios of LAMP assays based on the detection of a fluorescent intercalating-dye in an amplification product.

公开(公告)号：US20230143555A1

公开(公告)日：2023-05-11

申请号：US18046817

申请日：2022-10-14

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI ,  BAYLOR COLLEGE OF MEDICINE

Inventor： Sri KRISHNA ,  Marshall POSNER ,  Andrew SIKORA ,  Karen ANDERSON

IPC: A61K39/12 ,  A61P31/20 ,  A61P35/00

CPC classification number: A61K39/12 ,  A61P31/20 ,  A61P35/00 ,  A61K2039/80

Abstract: Embodiments of the present disclosure pertain generally to head and neck squamous cell carcinomas (HNSCCs) related to human papillomavirus subtype 16 (HPV16) infections. More particularly, the present disclosure provides novel immunogenic epitopes from HPV16 E2, E6 and E7 antigens restricted by common human leukocyte antigen (HLA) alleles for the diagnosis and treatment of HNSCC. The HPV16 epitopes identified in the present disclosure can be used in combination with blockade of HPV16+ HNSCC-specific checkpoints for targeted immunotherapy.