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公开(公告)号：US20040014091A1
公开(公告)日：2004-01-22
申请号：US10386401
申请日：2003-03-11
申请人： ATHENIX CORPORATION
IPC分类号： C12Q001/68 , G06F019/00 , G01N033/48 , G01N033/50
CPC分类号： G06F19/22 , C12Q1/6888 , C12Q1/689
摘要： Compositions and methods for rapid and highly efficient characterization of genetic diversity in organisms are provided. The methods involve rapid sequencing and characterization of extrachromosomal DNA, particularly plasmids, to identify and isolate useful nucleotide sequences. The method targets plasmid DNA and avoids repeated cloning and sequencing of the host chromosome, thus allowing one to focus on the genetic, elements carrying maximum genetic diversity. The method involves generating a library of extrachromosomal DNA clones, sequencing a portion of the clones, comparing the sequences against a database of existing DNA sequences, using an algorithm to select said novel nucleotide sequence based on the presence or absence of said portion in a database, and identification of at least one novel nucleotide sequence. The DNA sequence can also be translated in all six frames and the resulting amino acid sequences can be compared against a database of protein sequences. The integrated approach provides a rapid and efficient method to identify and isolate useful genes. Organisms of particular interest include, but are not limited to bacteria, fungi, algae, and the like. Compositions comprise a mini-cosmid vector comprising a stuffer fragment and at least one cos site.
摘要翻译： 提供了生物体遗传多样性快速高效表征的组成和方法。 该方法涉及染色体外DNA特别是质粒的快速测序和表征，以鉴定和分离有用的核苷酸序列。 该方法针对质粒DNA，避免宿主染色体的重复克隆和测序，从而允许人们将遗传因素集中在最大的遗传多样性上。 该方法包括产生染色体外DNA序列文库，测序一部分克隆，比较序列与现有DNA序列的数据库，使用算法根据数据库中所述部分的存在或不存在来选择所述新的核苷酸序列 ，以及鉴定至少一个新的核苷酸序列。 DNA序列也可以在所有六个帧中翻译，并将得到的氨基酸序列与蛋白质序列的数据库进行比较。 综合方法提供了一种快速有效的方法来鉴定和分离有用的基因。 特别感兴趣的生物体包括但不限于细菌，真菌，藻类等。 组合物包含迷你粘粒载体，其包含填充片段和至少一个cos位点。
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公开(公告)号：US20040250311A1
公开(公告)日：2004-12-09
申请号：US10781979
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： C12N015/82 , C07H021/04 , C12N015/87 , C12N015/09 , C07K014/00 , A01H005/10 , C12N005/10 , C12N015/63 , A01H005/00 , A61K039/395
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin and delta-endotoxin-associated polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin and delta-endotoxin-associated nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3, 5, and 7, and the nucleotide sequences set forth in SEQ ID NO:1, 2, 4, and 6, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素和δ-内毒素相关多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素和δ-内毒素相关的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3,5和7所示氨基酸序列的核苷酸序列，以及SEQ ID NO： 和6，以及其变体和片段。
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公开(公告)号：US20040231060A1
公开(公告)日：2004-11-25
申请号：US10795102
申请日：2004-03-05
申请人： Athenix Corporation
发明人： Jill Burdette , Brian Vande Berg , Brian Carr , Nicholas B. Duck , Michael G. Koziel , Nadine Carozzi , Paresma R. Patel
IPC分类号： D06M010/00
CPC分类号： C12P7/10 , C08H8/00 , C12N9/0006 , C12N9/18 , C12N9/20 , D21C5/005 , D21C9/16 , D21C11/0007 , Y02E50/16
摘要： Methods for hydrolyzing lignocellulose are provided, comprising contacting the lignocellulose with at least one chemical treatment. Methods for pretreating a lignocellulosic material comprising contacting the material with at least one chemical are also provided. Methods for liberating a substance such as an enzyme, a pharmaceutical, or a nutraceutical from plant material are also provided. These methods are more efficient, more economical, and less toxic than current methods.
摘要翻译： 提供水解木质纤维素的方法，包括使木质纤维素与至少一种化学处理接触。 还提供了用于预处理木质纤维素材料的方法，包括使材料与至少一种化学品接触。 还提供了用于从植物材料释放诸如酶，药物或营养品的物质的方法。 这些方法比目前的方法更有效，更经济，毒性更低。
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公开(公告)号：US20040216186A1
公开(公告)日：2004-10-28
申请号：US10783417
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： A01H001/00 , C07H021/04 , C12N015/82 , C12N005/04
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 and the nucleotide sequence set forth in SEQ ID NO:1, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列和SEQ ID NO：1所示的核苷酸序列，以及其变体和片段。
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公开(公告)号：US20040005674A1
公开(公告)日：2004-01-08
申请号：US10426111
申请日：2003-04-29
申请人： Athenix Corporation
IPC分类号： C12P019/02
CPC分类号： C12P19/14
摘要： Compositions and methods for biomass conversion are provided. Compositions comprise novel enzyme mixtures that can be used directly on lignocellulose substrate. Methods involve converting lignocellulosic biomass to free sugars and small oligosaccharides with enzymes that break down lignocellulose. Novel combinations of enzymes are provided that provide a synergistic release of sugars from plant biomass. Also provided are methods to identify enzymes, strains producing enzymes, or genes that encode enzymes capable of degrading lignocellulosic material to generate sugars.
摘要翻译： 提供了生物质转化的组合物和方法。 组合物包含可直接用于木质纤维素底物的新型酶混合物。 方法涉及将木质纤维素生物质转化为游离糖和小分子糖，以分解木质纤维素酶。 提供了新的酶组合，其提供来自植物生物质的糖的协同释放。 还提供了鉴定酶，产生酶的菌株或编码能够降解木质纤维素材料以产生糖的酶的基因的方法。
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公开(公告)号：US20040210965A1
公开(公告)日：2004-10-21
申请号：US10782570
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： A01H001/00 , C12N015/82 , C07H021/04 , C12N005/04
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:2 and 4 and the nucleotide sequences set forth in SEQ ID NOS:1 and 3, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2和4所示的氨基酸序列的核苷酸序列和SEQ ID NO：1和3所示的核苷酸序列，以及变体和片段 其中。
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公开(公告)号：US20040210964A1
公开(公告)日：2004-10-21
申请号：US10782096
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： A01H001/00 , C12N015/82 , C07H021/04 , C12N005/04
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6 and the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列和SEQ ID NO：1,3或5所示的核苷酸序列， 以及其变体和片段。
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公开(公告)号：US20040197917A1
公开(公告)日：2004-10-07
申请号：US10782141
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： C07H021/04 , A61K039/395 , C12N015/82 , C12N015/87 , A01H005/00 , C07K001/00
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or 5, and nucleotide sequences set forth in SEQ ID NO:1, 2, or 4, and variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3或5所示的氨基酸序列的核苷酸序列，以及SEQ ID NO：1,2或4所示的核苷酸序列，以及变体和 片段。
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公开(公告)号：US20040197916A1
公开(公告)日：2004-10-07
申请号：US10782020
申请日：2004-02-19
申请人： Athenix Corporation
IPC分类号： C12N015/82 , C07H021/04 , A61K039/395 , C12N015/63 , C07K014/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequences shown in SEQ ID NOS:3 and 5, and the nucleotide sequences set forth in SEQ ID NOS:1, 2, and 4, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：3和5所示的氨基酸序列的核苷酸序列和SEQ ID NO：1,2和4所示的核苷酸序列的分离的核酸分子，以及 作为其变体和片段。
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