公开(公告)号：US20040268433A1

公开(公告)日：2004-12-30

申请号：US10733407

申请日：2003-12-12

发明人： Joanne Elizabeth Burn ,  Richard Edward Williamson

IPC分类号： C12N015/82 ,  C12N015/87 ,  A01H005/00 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10

CPC分类号： C07K14/415 ,  C12N15/8246

摘要： The present invention provides novel genes involved in cellulose biosynthesis and methods using such genes to modulate cellulose biosynthesis in fiber-producing plants such as cotton. The invention also provides methods for identifying and isolating alleles of these genes in a population of fiber-producing plants that correlate with the quality of the produced fibers.

公开(公告)号：US20040255349A1

公开(公告)日：2004-12-16

申请号：US10169426

申请日：2003-01-31

发明人： Hiroshi Takatsuji ,  Meenu Kapoor

IPC分类号： C12N015/82 ,  C07H021/04 ,  C12N015/87 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N15/8214 ,  C12N15/823 ,  C12N15/8231 ,  C12N15/827 ,  Y02A40/146

摘要： Genomic DNA containing the promoter region of the pMADS3 gene was isolated, ligated to a reporter gene, and then introduced into petunia plants via an Agrobacterium-mediated method. Surprisingly, the resultant plants became double-flowered by conversion of stamens into petaloid structures.

摘要翻译： 分离含有pMADS3基因启动子区的基因组DNA，与报告基因连接，然后通过土壤杆菌介导法引入矮牵牛属植物。 令人惊讶的是，通过将雄蕊转化为花瓣状结构，所得植物变得双花。

公开(公告)号：US20040254364A1

公开(公告)日：2004-12-16

申请号：US10828919

申请日：2004-04-20

发明人： Michael J. Adang ,  Laura M. Kasman

IPC分类号： C12Q001/68 ,  C07H021/04 ,  C12N005/10 ,  C12N015/74

CPC分类号： C40B40/02 ,  C07H21/04 ,  C07K14/325 ,  C07K2319/00 ,  C12N15/1037

摘要： Disclosed herein are activated Bt toxins expressed in E. coli as a translational fusion with a phage coat protein of filamentous phage. Phage displaying this fusion protein were viable, infectious, and as lethal as pure toxin on a molar basis when fed to insects susceptible to native Bt toxins.

摘要翻译： 本文公开了在大肠杆菌中表达的活化的Bt毒素作为丝状噬菌体的噬菌体外壳蛋白的翻译融合物。 展示该融合蛋白的噬菌体在喂给易受天然Bt毒素的昆虫时，是以摩尔计存活，感染和致死的纯毒素。

公开(公告)号：US20040244078A1

公开(公告)日：2004-12-02

申请号：US10486376

申请日：2004-07-16

发明人： Alison Baker ,  Stephen Slocombe ,  Ian Graham

IPC分类号： C12N015/82 ,  C12Q001/68 ,  C12N015/87 ,  C12N015/63 ,  C12N005/10 ,  C12N015/29 ,  C07K014/00 ,  C12N015/09 ,  C07H021/04

CPC分类号： C07K14/415 ,  C12N15/8209 ,  C12N15/8247

摘要： A nucleic acid which encodes a peroxisomal fatty acid transporter, uses thereof and a method of genetic manipulation of peroxisomal fatty acid transport and/or Metabolism. The nucleic acid and its products are especially for use in regulation of peroxisomal fatty acid transport in plant and in controlling the spectrum of fatty acids which can be utilised by the plant.

摘要翻译： 编码过氧化物酶体脂肪酸转运蛋白的核酸，其用途和遗传操作过氧化物酶体脂肪酸转运和/或代谢的方法。 核酸及其产物特别用于调节植物中过氧化物酶体脂肪酸的转运和控制植物可利用的脂肪酸的光谱。

公开(公告)号：US20030207327A1

公开(公告)日：2003-11-06

申请号：US10260638

申请日：2002-09-27

发明人： Eric B. Kmiec ,  Michael C. Rice

IPC分类号： G01N033/53 ,  C12N001/18 ,  C12N005/10 ,  C12N005/06 ,  C12N005/08

CPC分类号： C12Q1/6876

摘要： Collections of cultured eukaryotic cells, particularly human cells, in which the cells are coisogenic at a common target locus, are provided. Particularly provided are collections of coisogenic cells that differ in genomic sequence by no more than 0.05%, excluding changes at the target locus, collections in which the coisogenic cells differ in genomic sequence by no more than 0.005%, excluding changes at the target locus, and collections in which the cells lack heterologous genetic elements within 10 kilobases of the coisogenic target locus. Kits comprising the cell collections, methods of making the collections, kits for making the collections, and methods of using the collections to facilitate pharmacogenomic analyses are presented. Preferred target loci at which the cells are coisogenic include genes that affect drug resistance, drug sensitivity, and/or drug metabolism.

摘要翻译： 提供了培养的真核细胞，特别是人细胞的集合，其中细胞在共同的靶标位点处共成孔。 特别提供的是基因组序列不同于不超过0.05％的不同基因组序列的集合，排除目标基因座处的变化，其中共基因细胞在基因组序列上不同不超过0.005％的集合，不包括目标基因座处的变化， 以及其中细胞在共生靶向基因座的10千碱基中缺少异源遗传元件的集合。 提出了包括细胞集合的组合，制备收集物的方法，用于制备收集物的试剂盒以及使用该组合来促进药物基因组学分析的方法。 细胞共成突变的优选靶基因包括影响耐药性，药物敏感性和/或药物代谢的基因。

公开(公告)号：US20030135877A1

公开(公告)日：2003-07-17

申请号：US10100294

申请日：2002-03-18

发明人： Heiko A. Haertel ,  Volker Mittendorf ,  Ruoying Chen ,  Karin J. Shank

IPC分类号： C12N015/82 ,  C12N015/87 ,  C12N015/29 ,  A01H005/00 ,  C12N005/04 ,  C12N005/10

CPC分类号： C07K14/415 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251

摘要： The present invention is directed to novel nucleic acid and amino acid sequences associated with the metabolism of seed storage compounds in plants. More particularly novel lipid metabolism protein (LMP) sequences are provided herein. Preferably, the seed storage compounds are lipids, fatty acids, starches or seed storage proteins.

公开(公告)号：US20020187520A1

公开(公告)日：2002-12-12

申请号：US09133766

申请日：1998-08-12

发明人： BIRGIT ANNA HELM ,  ANNE WILSON ,  DENISE MOREIRA-MACHADO

IPC分类号： G01N033/53 ,  G01N033/567 ,  G01N033/555 ,  C12N005/02 ,  C12N005/06 ,  C12N005/10 ,  C12Q001/02 ,  G01N033/566

CPC分类号： A61K31/70 ,  A61K38/04 ,  G01N33/5091 ,  G01N33/6854 ,  Y10S436/804 ,  Y10S436/811

摘要： The invention relates to the use of a secretor variant cell-line expressing the alpha moiety of human IgE binding protein to determine the allergic status of a given individual. Moreover, the cell-line is also used to provide an assay system for determining the allergenicity of substances and for subsequently providing therapeutic compostions which render said substances ineffective. In addition, the invention also relates to the use of said cell-line to determine the IgE independent irritancy of substances and compositions effective for attenuating the effects of said substances.

摘要翻译： 本发明涉及使用表达人IgE结合蛋白的α部分的分泌型变体细胞系来确定给定个体的过敏状态。 此外，细胞系还用于提供用于确定物质的变应原性的测定系统，并且随后提供使所述物质无效的治疗组合物。 此外，本发明还涉及所述细胞系用于确定有效减弱所述物质的作用的物质和组合物的IgE独立刺激性的用途。

公开(公告)号：US20020165385A1

公开(公告)日：2002-11-07

申请号：US09906209

申请日：2001-07-16

发明人： Stephen M. Allen ,  Karlene H. Butler ,  Thomas J. Carlson ,  Lawrence L. Ilag

IPC分类号： C12N015/29 ,  C12N015/82 ,  C12N005/04 ,  C12P021/02 ,  C12N005/10

CPC分类号： C12N9/90 ,  C12N15/8216 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8251

摘要： This invention relates to an isolated nucleic acid fragment encoding a plastidic phosphoglucomutase protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the plastidic phosphoglucomutase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the plastidic phosphoglucomutase in a transformed host cell.

摘要翻译： 本发明涉及编码质体磷酸果糖调蛋白酶蛋白的分离的核酸片段。 本发明还涉及在正义或反义方向上编码质体磷酸葡萄糖果糖转移酶的所有或大部分的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中质体磷酸果糖转化酶水平的改变。

公开(公告)号：US20020146821A1

公开(公告)日：2002-10-10

申请号：US09307824

申请日：1999-05-07

发明人： JUAN SANCHEZ-RAMOS ,  SHIJIE SONG ,  WILLIAM JANSSEN ,  PAUL SANBERG ,  THOMAS FREEMAN

IPC分类号： C12P013/14 ,  C12N005/06 ,  C12N005/10 ,  C12N005/08 ,  C12N005/00 ,  C12N005/02

CPC分类号： C12N5/0619 ,  A61K35/12 ,  A61K2035/124 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/385 ,  C12N2501/392 ,  C12N2502/08 ,  C12N2506/1353

摘要： Bone marrow stromal cells (BMSC) differentiate into neuron-like phenotypes in vitro and in vivo, engrafted into normal or denervated rat striatum. The BMSC did not remain localized to the site of the graft, but migrated throughout the brain and integrated into specific brain regions in various architectonic patterns. The most orderly integration of BMSC was in the laminar distribution of cerebellar Purkinje cells, where the BMSC-derived cells took on the Purkinje phenotype. The BMSC exhibited site-dependent differentiation and expressed several neuronal markers including neuron-specific nuclear protein, tyrosine hydroxylase and calbindin. BMSC can be used to target specific brain nuclei in strategies of neural repair and gene therapy.

摘要翻译： 骨髓基质细胞（BMSC）在体外和体内分化成神经元样表型，植入正常或去神经支配的大鼠纹状体。 BMSC没有保留在移植物的位点，而是迁移到整个大脑，并以各种建筑模式整合到特定的脑区域。 BMSC最有序的整合是在小脑浦肯野细胞的层流分布中，其中BMSC衍生的细胞采用浦肯野表型。 BMSC表现出位点依赖性分化，并表达了几种神经元标志物，包括神经元特异性核蛋白，酪氨酸羟化酶和钙结合蛋白。 BMSC可用于靶向神经修复和基因治疗策略中的特定脑核。

公开(公告)号：US20040268443A1

公开(公告)日：2004-12-30

申请号：US10834786

申请日：2004-04-29

发明人： Ray J. Wu ,  Ajay K. Garg ,  Ju-Kon Kim

IPC分类号： C12N015/82 ,  C12N015/87 ,  A01H005/00 ,  C12N015/09 ,  C12N015/63 ,  C12N005/10 ,  A01H005/10

CPC分类号： C12N15/8261 ,  Y02A40/146

摘要： An isolated nucleic acid construct including a nucleic acid molecule encoding a light-labile, phytochrome A, a light-inducible promoter which is 5null to the nucleic acid molecule encoding a light-labile, phytochrome A, and a terminator region which is 3null to the nucleic acid molecule encoding a light-labile, phytochrome A is disclosed. Methods for regulating a plant's canopy architecture and regulating a plant's seed yield, which involve transgenic plants or transgenic plant seeds including an isolated nucleic acid construct according to the present invention, are also disclosed.