公开(公告)号：US20240328990A1

公开(公告)日：2024-10-03

申请号：US18732126

申请日：2024-06-03

申请人： AXBIO INC.

发明人： Tao HONG ,  Igor Constantin IVANOV ,  Hui TIAN

IPC分类号： G01N27/327 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6876

CPC分类号： G01N27/3278 ,  C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6876

摘要： The present disclosure provides systems and methods for analyzing or identifying a target nucleic acid molecule. For example, a system of the present disclosure may comprise (a) a complex comprising a target nucleic acid molecule and a primer nucleic acid molecule, wherein the primer nucleic acid molecule comprises (i) a region that is complementary to a portion of the target nucleic acid molecule and (ii) an additional region that is non-complementary the target nucleic acid molecule, and (b) with said additional region having flown through a pore of a sensor, using said sensor to identify said additional region, thereby analyzing said target nucleic acid molecule. The sensor may be configured to detect one or more signals indicative of an impedance or impedance change in the sensor when the at least the portion of the target molecule is bound by the binding unit. The one or more signals may be usable to analyze or identify the target molecule.

公开(公告)号：US20240318246A1

公开(公告)日：2024-09-26

申请号：US18616750

申请日：2024-03-26

申请人： AXBIO INC.

发明人： Hui TIAN ,  Igor Constantin IVANOV ,  Vladimir Ivanovich BASHKIROV

IPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6855

CPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6855

摘要： The present disclosure provides methods for processing a nucleic acid sample. For example, a method of the present disclosure may simultaneously process two populations of nucleic acid (NA) templates for two different sequencing modalities: (i) a first population comprising circularized NA templates having a size smaller than a size threshold (e.g., smaller than 2 kilobase (kb)), and (ii) a second population comprising uncircularized NA templates having a size greater than or equal to the size threshold.

公开(公告)号：US20220396831A1

公开(公告)日：2022-12-15

申请号：US17587643

申请日：2022-01-28

申请人： AXBIO INC.

发明人： Igor Constantin IVANOV ,  Hui TIAN

IPC分类号： C12Q1/6869

摘要： The present disclosure provides systems and methods for analyzing or identifying a target molecule. For example, a system of the present disclosure may comprise (i) a sensing electrode, (ii) a binding unit coupled to the sensing electrode and configured to bind at least a portion of the target molecule, and (iii) a dielectric material coupled to the sensing electrode and covering at least a portion of a surface of the sensing electrode. The sensor may be configured to detect one or more signals indicative of an impedance or impedance change in the sensor when the at least the portion of the target molecule is bound by the binding unit. The one or more signals may be usable to analyze or identify the target molecule.

公开(公告)号：US20240076730A1

公开(公告)日：2024-03-07

申请号：US18164102

申请日：2023-02-03

申请人： AXBIO INC.

发明人： Suhua DENG ,  Vladimir Ivanovich BASHKIROV ,  Hui TIAN ,  Igor Constantin IVANOV

IPC分类号： C12Q1/6869 ,  C12Q1/6806

CPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides a method and systems for processing or analyzing a nucleic acid molecule. A method for processing or analyzing a double-stranded nucleic molecule may comprise providing the double-stranded nucleic acid molecule and a double-stranded adapter. The double-stranded adapter may comprise a nicking site within a sense strand or an anti-sense strand of the double-stranded adapter. The double-stranded adapter may then be coupled to the double-stranded nucleic acid molecule, and the double-stranded nucleic acid molecule coupled to the double-stranded adapter may be circularized to generate a circularized double-stranded nucleic acid molecule.

公开(公告)号：US20210139971A1

公开(公告)日：2021-05-13

申请号：US16953667

申请日：2020-11-20

申请人： AXBIO INC.

发明人： Suhua DENG ,  Vladimir Ivanovich BASHKIROV ,  Hui TIAN ,  Igor Constantin IVANOV

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides a method and systems for processing or analyzing a nucleic acid molecule. A method for processing or analyzing a double-stranded nucleic molecule may comprise providing the double-stranded nucleic acid molecule and a double-stranded adapter. The double-stranded adapter may comprise a nicking site within a sense strand or an anti-sense strand of the double-stranded adapter. The double-stranded adapter may then be coupled to the double-stranded nucleic acid molecule, and the double-stranded nucleic acid molecule coupled to the double-stranded adapter may be circularized to generate a circularized double-stranded nucleic acid molecule.