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公开(公告)号：US20100209987A1
公开(公告)日：2010-08-19
申请号：US12656821
申请日：2010-02-17
IPC分类号： C12N13/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
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公开(公告)号：US07060804B2
公开(公告)日：2006-06-13
申请号：US10182126
申请日：2001-01-22
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z′, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, α-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising such a complex or colloidal particles.
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3.发明授权Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途公开(公告)号：US07700744B2
公开(公告)日：2010-04-20
申请号：US11320574
申请日：2005-12-30
IPC分类号： A23J1/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。
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4.发明授权Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell 有权运送或储存蛋白质，病毒，原核细胞或真核细胞的方法公开(公告)号：US08093026B2
公开(公告)日：2012-01-10
申请号：US12656821
申请日：2010-02-17
IPC分类号： C12N13/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。
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5.发明申请Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途公开(公告)号：US20060105326A1
公开(公告)日：2006-05-18
申请号：US11320574
申请日：2005-12-30
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。
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