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    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    3.
    发明授权
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US07700744B2

    公开(公告)日：2010-04-20

    申请号：US11320574

    申请日：2005-12-30

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： A23J1/00

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell
    4.
    发明授权
    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell 有权
    运送或储存蛋白质，病毒，原核细胞或真核细胞的方法

    公开(公告)号：US08093026B2

    公开(公告)日：2012-01-10

    申请号：US12656821

    申请日：2010-02-17

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12N13/00

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    5.
    发明申请
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US20060105326A1

    公开(公告)日：2006-05-18

    申请号：US11320574

    申请日：2005-12-30

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N1/08

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Nucleotide sequence detection with signal amplification
    6.
    发明授权
    Nucleotide sequence detection with signal amplification 失效
    信号放大的核苷酸序列检测

    公开(公告)号：US6033853A

    公开(公告)日：2000-03-07

    申请号：US952397

    申请日：1997-01-08

    申请人： Thierry Delair ,  Abdelhamid Elaissari ,  Marie-Helene Charles ,  Bernard Mandrand

    发明人： Thierry Delair ,  Abdelhamid Elaissari ,  Marie-Helene Charles ,  Bernard Mandrand

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12Q1/6834 ,  C12Q1/682

    摘要： Kit for detecting a specific target nucleic acid sequence using a labeled probe with signal amplification, which comprises in containers (i) a probe labeled with a tracer (ii) a suspension of insoluble particles on which are immobilized at least one series of oligonucleotide units all of which are identical and contain at least one sequence that specifically hybridize with the target nucleic acid sequence and at one sequence that specifically hybridize with tracer labeled probe. A method using the kit is also described.

    摘要翻译： PCT No.PCT / FR97 / 00483 Sec。 371日期1998年1月8日 102（e）日期1998年1月8日PCT 1997年3月19日PCT公布。 公开号WO97 / 35031 日期1997年9月25日用于使用具有信号扩增的标记探针检测特异性靶核酸序列，其包含在容器中（i）用示踪剂标记的探针（ii）不溶性颗粒的悬浮液，其上固定有至少一种 一系列寡核苷酸单元都是相同的，并且含有至少一个与靶核酸序列特异性杂交的序列，以及与跟踪标记探针特异性杂交的一个序列。 还描述了使用该试剂盒的方法。