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    Reagent and method for the detection of a nucleotide sequence with signal amplification
    3.
    发明授权
    Reagent and method for the detection of a nucleotide sequence with signal amplification 失效
    用于检测具有信号放大的核苷酸序列的试剂和方法

    公开(公告)号：US6017707A

    公开(公告)日：2000-01-25

    申请号：US870730

    申请日：1997-06-06

    Applicant: Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    Inventor： Bernard Mandrand ,  Philippe Cros ,  Thierry Delair ,  Marie-Helene Charles ,  Marie-Noelle Erout ,  Christian Pichot

    IPC: C08G81/02 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12N15/00

    CPC classification number: C12Q1/6813 ,  C08G81/02 ,  C12Q1/682

    Abstract: Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.

    Abstract translation: 检测目的核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有 包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。

    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    4.
    发明授权
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US07700744B2

    公开(公告)日：2010-04-20

    申请号：US11320574

    申请日：2005-12-30

    Applicant: Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    Inventor： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC: A23J1/00

    CPC classification number: C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    Abstract: The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    Abstract translation: 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Method for isolating a target biological material, capture phase, detecting phase and reagent containing them
    6.
    发明授权
    Method for isolating a target biological material, capture phase, detecting phase and reagent containing them 有权
    用于分离靶生物材料，捕获相，检测相和含有它们的试剂的方法

    公开(公告)号：US06632615B2

    公开(公告)日：2003-10-14

    申请号：US09230846

    申请日：1999-04-21

    Applicant: Francois Mallet ,  Thierry DeLair ,  Catherine Ladaviere ,  Armelle Novelli-Rousseau ,  Marie-Helene Charles

    Inventor： Francois Mallet ,  Thierry DeLair ,  Catherine Ladaviere ,  Armelle Novelli-Rousseau ,  Marie-Helene Charles

    IPC: G01N3353

    CPC classification number: G01N33/54393 ,  C12N15/62 ,  C12N15/87

    Abstract: The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognizing or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues, the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.

    Abstract translation: 本发明涉及一种用于分离样品中包含的靶生物材料的方法，其包括提供包含至少具有反应性功能的有机分子和至少能够识别或结合，特异性和直接或间接的蛋白质材料的捕获相 与目标生物材料一起，所述蛋白质材料具有与有机分子反应性功能的特定共价结合位点，由至少包含至少六个相邻赖氨酸或赖氨酸衍生物残基的标签组成，该方法包括使所述靶生物材料 至少有捕获阶段; 并检测固定在捕获相上的靶生物材料：本发明还涉及捕获和检测阶段以及含有它们的试剂。

    Cationic polymers, complexes associating said cationic polymers with therapeutically active substances comprising at least a negative charge, in particular nucleic acids, and their use in gene therapy
    9.
    发明授权
    Cationic polymers, complexes associating said cationic polymers with therapeutically active substances comprising at least a negative charge, in particular nucleic acids, and their use in gene therapy 失效
    将阳离子聚合物与至少包含负电荷，特别是核酸的治疗活性物质结合的阳离子聚合物，以及它们在基因治疗中的用途

    公开(公告)号：US06407178B1

    公开(公告)日：2002-06-18

    申请号：US09463249

    申请日：2000-01-21

    Applicant: Hanno V. J. Kolbe ,  Otmane Boussif ,  Thierry Delair ,  Laurent Veron

    Inventor： Hanno V. J. Kolbe ,  Otmane Boussif ,  Thierry Delair ,  Laurent Veron

    IPC: A01N4304

    CPC classification number: C12N15/87 ,  A61K47/645 ,  A61K47/6883 ,  A61K48/00 ,  C08F8/00 ,  C08F126/02 ,  C08F26/02

    Abstract: The invention concerns novel cationic polymers of formula (I) in which n is a whole number between 0 and 5 and p is a whole number between 2 and 20,000, more particularly p ranges between 10 and 18,000, and advantageously between 200 and 1,000, characterized in that: at least 10%, advantageously 30 to 80%, preferably 70%, of the free NH2 functions are substituted by identical or different hydrophilic R groups; said cationic polymer can further comprise at least a targeting element associated covalently or not with the free NH2 functions and/or said hydrophilic R groups provided that said cationic: polymer contains at least 20%, preferably at least 30%, of free NH2 functions. The invention also concerns a complex comprising at least said cationic polymer associated with at least a therapeutically active substance, in particular a nucleic acid comprising at least a negative charge. The invention is useful for transferring a nucleic acid into target cells, in particular for gene therapy.

    Abstract translation: 本发明涉及式（I）的新型阳离子聚合物，其中n是0和5之间的整数，p是2和20,000之间的整数，更特别是p在10和18,000之间，有利地在200和1,000之间，其特征在于 其中：至少10％，有利地30至80％，优选70％的游离NH 2官能团被相同或不同的亲水性R基团取代; 所述阳离子聚合物可进一步包含与游离NH 2官能团和/或所述亲水性R基团共价或不共价键合的至少一种靶向元件，条件是所述阳离子：聚合物含有至少20％，优选至少30％的游离NH 2官能团。 本发明还涉及至少包含与至少一种治疗活性物质相关的阳离子聚合物的复合物，特别是包含至少一种负电荷的核酸。 本发明可用于将核酸转移到靶细胞中，特别是用于基因治疗。

    Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological molecules
    10.
    发明授权
    Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological molecules 失效
    衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用

    公开(公告)号：US5439972A

    公开(公告)日：1995-08-08

    申请号：US321447

    申请日：1994-10-12

    Applicant: Marie-Helene Charles ,  Thierry Delair ,  Monique Jaubert ,  Bernard F. Mandrand

    Inventor： Marie-Helene Charles ,  Thierry Delair ,  Monique Jaubert ,  Bernard F. Mandrand

    IPC: C07K16/18 ,  C08F8/00 ,  C08F8/30 ,  C08F8/34 ,  G01N33/543 ,  G01N33/545

    CPC classification number: G01N33/545 ,  C07K16/18 ,  C08F8/30 ,  C08F8/34 ,  G01N33/54313 ,  G01N33/54353 ,  Y10S530/815

    Abstract: Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological moleculesWater-soluble compound derived from a homopolymer or copolymer of maleic anhydride having available anhydride functional groups and hydrolyzed anhydride functional groups, wherein the hydrolyzed anhydride functional groups consist of carboxyl functional groups and functional groups derived from carboxyl functional groups carrying a residue of a compound corresponding to the formula I:C.sub.x H.sub.y A.sub.z O.sub.tin which:- A is the nitrogen atom of a primary, secondary or tertiary amine functional group or the sulfur atom of a thiol functional group,- x, z and t, independently of each other, are non-zero integers, and- y is a non-zero integer, not less than 5 when A is a nitrogen atom and not less than 4 when A is a sulfur atom.

    Abstract translation: 衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用衍生自具有可获得的酸酐官能团的马来酸酐和水解酸酐官能团的均聚物或共聚物的水溶性化合物，其中 水解酸酐官能团由羧基官能团和衍生自羧基官能团的官能团组成，所述羧基官能团带有对应于式I的化合物的残基：C x H y A z O t，其中：A是伯，仲或叔胺官能团的氮原子或 硫醇官能团的硫原子-x，z和t彼此独立地是非零整数，并且-Y是非零整数，当A是氮原子时不小于5 当A为硫原子时，超过4。

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