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16 条记录 (耗时 0.022 秒)

    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell
    7.
    发明授权
    Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell 有权
    运送或储存蛋白质，病毒，原核细胞或真核细胞的方法

    公开(公告)号：US08093026B2

    公开(公告)日：2012-01-10

    申请号：US12656821

    申请日：2010-02-17

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12N13/00

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
    9.
    发明申请
    Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权
    用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途

    公开(公告)号：US20060105326A1

    公开(公告)日：2006-05-18

    申请号：US11320574

    申请日：2005-12-30

    申请人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    发明人： Abdelhamid Elaissari ,  Bernard Mandrand ,  Thierry Delair ,  Doran Spencer ,  Ahmed Arkis

    IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N1/08

    CPC分类号： C12N15/1013 ,  C07K1/22 ,  G01N33/5434

    摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.

    摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。

    Nucleic acid isolation
    10.
    发明授权
    Nucleic acid isolation 失效
    核酸分离

    公开(公告)号：US06737235B1

    公开(公告)日：2004-05-18

    申请号：US08945731

    申请日：1997-11-10

    申请人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    发明人： Philippe Cros ,  Abdelhamid Elaissari ,  Claude Mabilat ,  Christian Pichot ,  Marc Rodrigue ,  Lise Santoro

    IPC分类号： C07H2104

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12Q1/6806

    摘要： A method for aqueous phase nucleic acid isolation from a sample, comprising a step of nucleic acid adsorption on a particulate substrate, is disclosed. The method comprises an adsorption reagent preparation step (a) that includes a sol consisting of a aqueous continuous phase and a dispersed particulate substrate phase including a functionalized particulate polymer prepared by polymerizing (1) a first water-soluble acrylamide or acrylamide derivative monomer, (2) at least one cross-linking agent and (3) at least one second water-soluble, cationic and functional monomer, said polymer having a predetermined lower critical solubility temperature (LCST) of 25-45° C.; a contact step (b) wherein the adsorption reagent is contacted with the sample containing the nucleic acid; an adsorption step (c) wherein, to carry out the contact step (b), at least one parameter is selected for the reaction medium, said parameters being a pH no higher than 7, an ionic strength no higher than 10−2 M, and a temperature lower than the polymer LCST; a separation step (d) wherein the dispersed phase is separated from the continuous phase, optionally after it has been observed that adsorption has occurred; and a desorption step (e) wherein the nucleic acid is desorbed from the particulate substrate by increasing the ionic strength until an ionic strength higher than 10−2 M is achieved.

    摘要翻译： 公开了一种从样品中分离水相核酸的方法，包括在颗粒基质上的核酸吸附步骤。 该方法包括一种吸附剂制备步骤（a），其包括由水性连续相组成的溶胶和包含通过聚合（1）第一水溶性丙烯酰胺或丙烯酰胺衍生物单体制备的官能化颗粒聚合物的分散颗粒基质相（ 2）至少一种交联剂和（3）至少一种第二水溶性阳离子和功能性单体，所述聚合物具有25-45℃的预定的较低临界溶解度温度（LCST）。 接触步骤（b），其中所述吸附剂与含有所述核酸的样品接触; 吸附步骤（c）其中，为了进行接触步骤（b），为反应介质选择至少一个参数，所述参数为不高于7的pH，不高于10 -2的离子强度 M，温度低于聚合物LCST; 分离步骤（d），其中分散相与连续相分离，任选地在已经观察到发生吸附之后; 和解吸步骤（e），其中通过增加离子强度直到达到高于10μM的离子强度，从核粒子脱离。